Computational identification of fertility functions of bovine Reprimo gene

Improvement in fertility is possible through gene assisted selection. Cattle fertility and genes underlying it should be thoroughly studied and exploited to find solution to declining cattle fertility. Reprimo (RPRM) gene is a pleiotropic gene involved in suppression of cancer, regulation of mitotic cell cycle, cell cycle arrest and regulation of survival. Comparison of protein tertiary structures is important in inferring functional characteristics of new proteins. This study used computational approach to identify some fertility functions of bovine RPRM gene using motif prediction and protein structure comparison. Amino acid sequences of bovine RPRM gene and some other cattle fertility genes were retrieved from GenBank. Motifs in the amino acid sequence of bovine RPRM gene were predicted using PROSITE software. The domain structure of bovine RPRM protein was predicted using simple modular architecture research tool (SMART). Protein tertiary structures (3D structures) of bovine RPRM gene and other cattle fertility genes were predicted with Phyre2 software. To have structural and functional similarity, it has been found that protein structure after superimposition should have Root Mean Square Deviation (RMSD) value less than or equal to 2Å. The protein 3D structures of other cattle fertility genes were superposed against the protein 3D structure of bovine RPRM gene using SuperPose web server and the proteins with RMSD value of 2Å or less were predicted as proteins with similar functions and structures as bovine RPRM gene. The predicted motifs (N-glycosylation site, N-myristoylation site, and cAMP and cGMP-dependent protein kinase phosphorylation site) and protein structure comparison revealed that, bovine RPRM gene and bovine growth hormone gene have the same fertility function with alpha carbon and backbone root mean square deviations of 1.94 Å and 1.81 Å, respectively. It follows therefore that other fertility functions of bovine RPRM gene included sexual maturation, steroidogenesis, gametogenesis, gonadal differentiation and gonadotrophin secretion which are the functions of growth hormone gene.Keywords: Reprimo gene, fertility, growth hormone gene, motifs, root mean squaredeviatio

Effect of fibre and digestible energy levels on retail cuts, gut characteristics and morphology of growing rabbits

The retail cuts, gut characteristics and morphology of rabbits fed with varying dietary fibre and digestible energy (DE) levels were measured in a 70-d feeding trial using 135 weaner rabbits. The experiment was designed as a 3 × 3 factorial arrangement of treatments having 3 levels of dietary fibre (low, optimum and high) and 3 levels of DE (low, optimum and high). The rabbits were allotted to 9 dietary groups consisting of 15 rabbits each. Main effect of fibre and DE showed that dressing out percentage (P < 0.05), chilled carcass weight (P < 0.05) and reference carcass weight (P < 0.01) increased with increasing inclusion levels of fibre. Rabbits fed with (430 – 456 g/kg NDF, 249 - 253 g/kg ADF) had the highest (P < 0.05) weights of thoracic cage, loins, hind part, small intestine, empty caecum and highest (P < 0.05) villus height in the duodenum and jejunum. Rabbits fed (10.5 – 11 MJ/kg) diets had the least (P < 0.05) chilled and reference carcass weight. Interaction effect of fibre and DE showed that rabbit fed (445.70g/kgNDF, 252.10g/kgADF and 8.01MJ/Kg) diet recorded the highest (P < 0.05) chilled carcass weight. Rabbit fed with low fibre (249 – 258 g/kg NDF, 149 - 157 g/kg ADF) diets not withstanding the level of DE had low (P < 0.05) chilled and reference carcass weight. The villus height of the duodenum and crypt depth of the jejunum increased (P < 0.05) with increasing level of dietary fibre irrespective of the level of digestible energy. In conclusion, feeding of (440.41 g/kg NDF, 250.09 g/kg ADF, 10.52MJ/kg) diets to rabbits favoured improved retail cuts, gut characteristics and gut morphology.Keywords: Weaner rabbits; Carcass yield; Organ weights; Gut characteristics; Gut morpholog

Effect of fibre and digestible energy levels on growth performance, apparent nutrient digestibility and caecal fermentation of growing rabbits

A seventy-day feeding trial was conducted to investigate the effect of dietary fibre and digestible energy (DE) level on growth performance, apparent nutrient digestibility and caecal fermentation of growing rabbits. The experiment was laid out in a 3 × 3 factorial arrangement of 3 levels of fibre (low (249 – 258 g/kg NDF, 149 - 157 g/kg ADF), optimum (349 – 381 g/kg NDF, 188 - 193 g/kg ADF) and high (430 – 456 g/kg NDF, 249 - 253 g/kg ADF)) and 3 levels of DE (low (8 – 8.5 MJ/kg), optimum (10.5 – 11 MJ/kg) and high (12 – 12.30 MJ/kg)). A total of one hundred and thirty five weaner rabbits were allotted to 9 dietary groups of 15 rabbits each. Main effect of fibre showed that the final live weight and weight gain of rabbits increased (P < 0.01) while Ca retention reduced (P < 0.001) with increasing dietary fibre level. Rabbits fed (249 – 258 g/kg NDF, 149 - 157 g/kg ADF), had the worst (P < 0.05) feed to gain ratio. Rabbits fed (349 – 381 g/kg NDF, 188 - 193 g/kg ADF) had the highest apparent dry matter (P < 0.05), ether extract (P < 0.05), NDF (P < 0.001), ADF (P < 0.001) and organic matter digestibility (P < 0.001). Main effect of DE showed that rabbits fed high DE recorded the highest (P < 0.05) weight gain. Highest (P < 0.01) apparent dry matter, crude protein, ash, NDF, ADF, organic matter digestibility and Ca retention were recorded with rabbits fed optimum DE diet. Rabbits(445.70g/kgNDF, 252.10g/kgADF and 8.01MJ/Kg) recorded the highest (P < 0.001) final live weight, weight gain and feed intake (P<0.05) while rabbits fed (249 g/kg NDF, 149 g/kg ADF and 8.17MJ/Kg) had the least (P < 0.001) final live weight, weight gain, apparent dry matter digestibility, NDF and ADF digestibility. Highest (P<0.001) NDF digestibility was obtained with rabbits fed (370.79 g/kg NDF, 189.61 g/kg ADF and 10.84MJ/kg). Not withstanding the DE level of the diets, rabbits fed with (430 – 456 g/kg NDF, 249 - 253 g/kg ADF) recorded the highest (P<0.05) total VFA and acetic acid concentration. Rabbits fed (445.7g/KgNDF, 250.09g/KgADF and 10.52MJ/kg) recorded the best growth performance, improved caecal fermentation with no adverse effect on apparent nutrient digestibility.Keywords: Growing rabbits; Dietary fibre; Digestible energy; Caecal fermentatio

Frequencies of Some Morphological Features in Indigenous Chickens of South- Western Nigeria

Morphological features of chicken genetic resources must be carefully identified and considered in developing breeding strategies. The study was carried out to determine the frequencies of morphological features in indigenous chickens of South-Western Nigeria, using Lagos, Ogun, Osun and Oyo States as case study. Five major chicken markets (Mushin in Lagos State, Itoku and Ipokia in Ogun State, Olu-ode in Osun State and Molete in Oyo State) were used as study locations and three thousand, three hundred and thirteen chickens were observed in the five locations. Ipokia had the highest number (1,549) of chickens and Molete had the least (307) number of chickens. The traits observed included: feather structure, feather distribution, feather morphology, plumage colour, ear lobe colour, skin colour and shank colour. The data collected were subjected to descriptive statistics and inferential statistical analysis was also done for the results using chi-square test. It was observed that for those traits that recorded significant variation, there had been selection for these traits due to the high market value attached to them by attaching preference to some of its variants which were observed. Knowledge and well documentation of the potentials of indigenous chickens can help provide crucial information for a comprehensive breeding policy and full utilization of the animal.Keywords: Characterization, conservation, indigenous chickens, morphological feature

Effect of chicken genotype on growth performance and feed consumption in the development of broiler lines

This experiment was conducted to assess the effect of chicken genotype on the growth performance, feed intake and feed efficiency of the progenies resulting from pure, straight and reciprocal cross of Giriraja (Gr) and Alpha chickens. Data obtained on body weight, body length, breast girth, keel length, feed intake and feed efficiency were analyzed using general linear models analysis of variance. Generally, all growth traits and feed consumption characteristics were significantly affected (P < 0.05) by chicken genotype. Growth traits and feed intake increased as birds advanced in age. Giriraja X Giriraja purebred chickens had the highest body weight, breast girth, feed intake and feed conversion efficiency through out the experimental period. However, the progenies resulting from the reciprocal crosses of Alpha X Gr had significant higher body weight and keel. Also, the feed conversion efficiency of the reciprocal crosses were better than the dihybrid cross progenies. The results therefore suggest that the superiority exhibited by the Giriraja could be utilized to improve the growth performance of the indigenous chicken thereby speed up desirable progress in the improvement of the indigenous broiler line.Key words: Chicken, Crossbred, Feed consumption, Genotype, Growth trait, Purebre

Morphological characterization and evaluation of heat tolerance traits in Nigerian goat breeds

There are three main breeds of goat in Nigeria: The West African Dwarf (WAD), the Red Sokoto and the Sahel. Goats are renowned for their hardiness and can survive in most environments. The goats sampling was carried out from farms and markets across different states in Nigeria, according to the geographical distribution of the breeds published by Blench (1999). This experiment was carried out to analyse morphological variability among Nigerian goats. A total of 318 goats spread across the three Nigerian breeds were used in the following populations (57 Sahel, 187 Red Sokoto and 137 West African Dwarf). Data were collected on body weight, linear body measurements and heat tolerance traits and were subjected to analysis of variance using the fixed effects of breed, sex and location. Results showed that breed, sex and location significantly (P<0.05) affected all the linear body measurements and heat tolerance traits. The Sahel breed had the highest values in most of the measured traits compared to the West African Dwarf and the Red Sokoto breeds of goat. It was observed that goats from the northern part of Nigeria were heavier than their southern counterparts, and this can be likened to their genetic makeup and the extent of its interaction with the environment. The results of the study confirmed the population structure of Nigerian goat breeds and showed that they possess enough diversity to enhance genetic improvement for higher survival and productivity.Keywords: Goat, Breed, Traits, Respiratory rate and Pulse rat