NUTRIENT TRANSFER IN VESICULAR-ARBUSCULAR MYCORRHIZAS: A NEW MODEL BASED ON THE DISTRIBUTION OF ATPases ON FUNGAL AND PLANT MEMBRANES

In this paper we review the membrane transport processes that are involved in the transfer of mineral nutrients and organic  carbon between  the  symbiotic partners  in mycorrhizas.  In particular, we  reassess  the prevailing hypothesis that transfer in vesicular-arbuscular (VA) mycorrhizas occurs  simultaneously and bidirectionally across the same interface and that arbuscules are the main sites of transfer. Using cytochemical techniques, we and our collaborators have reexamined the distribution of ATPases in the arbuscular and intercellular hyphal interfaces in VA mycorrhizas formed between roots of Allium cepa (onion) and the fungus Glomus intraradices. The results showed that H+-ATPases have different localisation on plant and fungal membranes in arbuscular and hyphal interfaces (Gianinazzi-Pearson et al. 1991). While some arbuscular interfaces had H+-ATPase activity on both fungal and plant membranes, in most cases the fungal membrane lacked this activity.  In contrast, the plasma membranes of intercellular hyphae always had H+-ATPase and the adjacent root cells did not. This suggests that the different interfaces in a VA mycorrhiza may have different functions. We propose that passive loss of P from the arbuscules is associated with active uptake by the energised (ATPase-bearing) plant membrane and that passive loss of carbohydrate from the root cells is followed by active uptake by the intercellular hyphae. If this model is correct, then variations in "mycorrhizal efficiency" (i.e. the extent to which mycorrhizal plants grow better than non-mycorrhizal controls) might be determined by differences in the numbers of active arbuscules as a proportion of the total fungal biomass within the root. As  a  first  step  towards  investigating  this  possibility, we  have  developed methods  for measuring  the surface areas of arbuscular and hyphal interfaces in different fungus-host combinations, Glomus spp./ Allium porrum (leek). We have also measured fluxes of P from fungus to plant and have been able to partition these between the arbuscular and total (arbuscular plus hyphal) interfaces.  The implications of this work, and suggestions for future investigations of the molecular mechanisms involved in nutrient transfer in mycorrhizas, are discussed.Key words: Mycorrhizas/Glomus intraradices/ATPases/Allium cepa

Validation of a Rodent Model of Chemotherapy-Induced Peripheral Neuropathy Using Oxaliplatin

poster abstractOxaliplatin (OXPL) is one of the most widely used and effective chemotherapeutic agents for colorectal cancer. However, the drug therapy is accompanied by severe dose-limiting off-target effects including tingling, burning pain and mechanical allodynia in the extremities of patients; together these symptomology is better known as chemotherapy-induced peripheral neuropathy (CIPN). The underlying pathophysiological mechanisms of CIPN are poorly understood and current therapeutic options only serve to alleviate the symptoms rather than prevent CIPN. To better understand mechanisms of OXPL-induced CIPN (OXPLN), we exposed adult female Sprague-Dawley rats to four intraperitoneal injections of vehicle or OXPL on alternative days. Behavioral results showed that thermal sensitivity failed to be affected by the OXPL. In contrast, the magnitude of mechanical allodynia increased such that the baseline withdrawal threshold for drug-treated animals was significantly lower than that for unprimed animals. Application of OXPL to afferent sensory neurons produced an increased amplitude and duration of compound action potentials that could be reversed with the voltage-gated sodium channel blocker, carbamazepine (CBZ). Astroglial and microglial markers glial fibrillary acidic protein (GFAP) and Iba-1 were imaged to examine glial reactivity in OXPLN at day 14. Microglia were not activated following OXPL whereas astrocytes exhibited increased GFAP fluorescence which paralleled OXPLN. Activation of astrocytes was prevented by co-administration of CBZ. These observations suggest that CBZ may serve to diminish OXPLN in the patient population. Oxaliplatin (OXPL) is one of the most widely used and effective chemotherapeutic agents for colorectal cancer. However, the drug therapy is accompanied by severe dose-limiting off-target effects including tingling, burning pain and mechanical allodynia in the extremities of patients; together these symptomology is better known as chemotherapy-induced peripheral neuropathy (CIPN). The underlying pathophysiological mechanisms of CIPN are poorly understood and current therapeutic options only serve to alleviate the symptoms rather than prevent CIPN. To better understand mechanisms of OXPL-induced CIPN (OXPLN), we exposed adult female Sprague-Dawley rats to four intraperitoneal injections of vehicle or OXPL on alternative days. Behavioral results showed that thermal sensitivity failed to be affected by the OXPL. In contrast, the magnitude of mechanical allodynia increased such that the baseline withdrawal threshold for drug-treated animals was significantly lower than that for unprimed animals. Application of OXPL to afferent sensory neurons produced an increased amplitude and duration of compound action potentials that could be reversed with the voltage-gated sodium channel blocker, carbamazepine (CBZ). Astroglial and microglial markers glial fibrillary acidic protein (GFAP) and Iba-1 were imaged to examine glial reactivity in OXPLN at day 14. Microglia were not activated following OXPL whereas astrocytes exhibited increased GFAP fluorescence which paralleled OXPLN. Activation of astrocytes was prevented by co-administration of CBZ. These observations suggest that CBZ may serve to diminish OXPLN in the patient population

Elastic and Mechanical Properties of Human Dentin

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/67340/2/10.1177_00220345580370041801.pd

Strength Properties of Waxes at Various Temperatures and Their Practical Application

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/66922/2/10.1177_00220345670460013101.pd

Study of Effect on Teeth of Intermittent Fluoridation of a Community Water Supply

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/67913/2/10.1177_00220345530320011601.pd

Prospects of Open Charm Production at GSI-FAIR and J-PARC

We present a detailed phenomenological study of the prospects of open charm physics at the future $\bar{p}p$ and $pp$ facilities GSI-FAIR and J-PARC, respectively. In particular, we concentrate on differential cross sections and the charge and longitudinal double-spin asymmetries at next-to-leading order accuracy. Theoretical uncertainties for the proposed observables are estimated by varying the charm quark mass and the renormalization and factorization scales.Comment: 11 pages, 13 figure

Comparison between variable flavor number schemes for charm quark electroproduction

Where appropriate, the abbreviation 'VFNS' is replaced by 'CSN' to indicate the scheme using massive heavy quark coefficient functions proposed in this paper. The text below Eq. (2.13) and between Eqs. (2.33) and (2.36) has been considerably changed.Comment: 64 pages, LaTeX, 16 Postscript figure

Self-Interactions in the Spacetime of a Scalar-Tensor Cosmic String

We study the effect of the geometry and topology of a scalar-tensor cosmic string space-time on the electric and magnetic fields of line sources. It is shown that the dilatonic coupling of the gravity induces effects along the string comparable to a current flow allowing for forbidden regions near the string.Comment: 7 pages, 2 figures. Revised version to appear in the Phys. Lett.

Differential Thermal Analysis of Commercial and Dental Waxes

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/67104/2/10.1177_00220345670460051701.pd