Global Distribution of Polaromonas Phylotypes - Evidence for a Highly Successful Dispersal Capacity

Bacteria from the genus Polaromonas are dominant phylotypes in clone libraries and culture collections from polar and high-elevation environments. Although Polaromonas has been found on six continents, we do not know if the same phylotypes exist in all locations or if they exhibit genetic isolation by distance patterns. To examine their biogeographic distribution, we analyzed all available, long-read 16S rRNA gene sequences of Polaromonas phylotypes from glacial and periglacial environments across the globe. Using genetic isolation by geographic distance analyses, including Mantel tests and Mantel correlograms, we found that Polaromonas phylotypes are globally distributed showing weak isolation by distance patterns at global scales. More focused analyses using discrete, equally sampled distances classes, revealed that only two distance classes (out of 12 total) showed significant spatial structuring. Overall, our analyses show that most Polaromonas phylotypes are truly globally distributed, but that some, as yet unknown, environmental variable may be selecting for unique phylotypes at a minority of our global sites. Analyses of aerobiological and genomic data suggest that Polaromonas phylotypes are globally distributed as dormant cells through high-elevation air currents; Polaromonas phylotypes are common in air and snow samples from high altitudes, and a glacial-ice metagenome and the two sequenced Polaromonas genomes contain the gene hipA, suggesting that Polaromonas can form dormant cells

Long-term spatial and temporal trends in frost indices in Kansas, USA

Citation: Anandhi, A., Perumal, S., Gowda, P.H. et al. Climatic Change (2013) 120: 169. https://doi.org/10.1007/s10584-013-0794-4Frost indices such as number of frost days (nFDs), number of frost-free days (nFFDs), last spring freeze (LSF), first fall freeze (FFF), and growing-season length (GSL) were calculated using daily minimum air temperature (T[subscript min]) from 23 centennial weather stations across Kansas during four time periods (through 1919, 1920–1949, 1950–1979, and 1980–2009). A frost day is defined as a day with T[subscript min] < 0 °C. The long- and short-term trends in frost indices were analyzed at monthly, seasonal, and annual timescales. Probability of occurrence of the indices was analyzed at 5 %, 25 %, 50 %, 75 %, and 95 %. Results indicated a general increase in T[subscript min] from 1900 through 2009 causing a decrease in nFDs. LSF and FFF occurred earlier and later than normal in the year, respectively, thereby resulting in an increase in GSL. In general, northwest Kansas recorded the greatest nFD and lowest T[subscript min], whereas southeast Kansas had the lowest nFD and highest T[subscript min]; however, the magnitude of the trends in these indices varied with location, time period, and time scales. Based on the long-term records in most stations, LSF occurred earlier by 0.1–1.9 days/decade, FFF occurred later by 0.2–0.9 day/decade, and GSL was longer by 0.1–2.5 day/decade. At the 50 % probability level, Independence in the south-eastern part of Kansas had the earliest LSF (6 April), latest FFF (29 October) and longest GSL (207 days). Oberlin (north-western Kansas) recorded the shortest GSL (156 days) and earliest FFF (7 October) had the latest LSF (2 May) at the 50 % probability level. A positive correlation was observed for combinations of indices (LSF and GSL) and elevation, whereas a negative correlation was found between FFF and elevation