20 research outputs found
On what scales can GOSAT flux inversions constrain anomalies in terrestrial ecosystems?
This is the final version. Available on open access from European Geosciences Union via the DOI in this recordData availability.
CarbonTracker CT2016 results were provided by NOAA ESRL, Boulder, Colorado, USA, from the website at https://www.esrl.noaa.gov/gmd/ccgg/carbontracker/ (National Oceanic and Atmospheric Administration (NOAA) Earth System Laboratory (ESRL), 2019a). CASA GFED 4.1 and CASA CMS NEE fluxes were also downloaded from the CT2016 website. The GOSAT L4 product and VISIT NEE were downloaded from the GOSAT Data Archive Service (https://data2.gosat.nies.go.jp; NIES, 2019). The Dai Global Palmer Drought Severity Index was downloaded from the Research Data Archive at the National Center for Atmospheric Research, Computational and Information Systems Laboratory (https://doi.org/10.5065/D6QF8R93; Dai, 2017). NASA GOME-2 SIF products were obtained from the Aura Validation Data Center (https://avdc.gsfc.nasa.gov/; Aura Validation Data Center, 2019). FLUXCOM products were obtained from the data portal of the Max Planck Institute for Biochemistry (https://www.bgc-jena.mpg.de/geodb/projects/Home.php.; Max Plank Institue for Biogeochemistry, 2019). MERRA-2 products were downloaded from MDISC (https://gmao.gsfc.nasa.gov/reanalysis/MERRA-2/; Global Modeling and Assimilation Office, 2019), managed by the NASA Goddard Earth Sciences (GES) Data and Information Services Center (DISC). The GEOS-Chem forward and adjoint models are freely available to the public. Instructions for downloading and running the models can be found at http://wiki.seas.harvard.edu/geos-chem (Atmospheric Chemistry Modeling Group at Harvard University , 2019). ACOS GOSAT lite files were obtained from the CO2 Virtual Science Data Environment (https://co2.jpl.nasa.gov/; Jet Propulsion Laboratory, California Institute of Technology, 2019). The SST anomalies were downloaded from the National Oceanic and Atmospheric Administration (NOAA) Earth System Research Laboratory (ESRL) website (https://www.esrl.noaa.gov; National Oceanic and Atmospheric Administration (NOAA) Earth System Laboratory (ESRL), 2019b).Interannual variations in temperature and precipitation impact the carbon balance of terrestrial ecosystems, leaving an imprint in atmospheric CO2. Quantifying the impact of climate anomalies on the net ecosystem exchange (NEE) of terrestrial ecosystems can provide a constraint to evaluate terrestrial biosphere models against and may provide an emergent constraint on the response of terrestrial ecosystems to climate change. We investigate the spatial scales over which interannual variability in NEE can be constrained using atmospheric CO2 observations from the Greenhouse Gases Observing Satellite (GOSAT). NEE anomalies are calculated by performing a series of inversion analyses using the GEOS-Chem adjoint model to assimilate GOSAT observations. Monthly NEE anomalies are compared to "proxies", variables that are associated with anomalies in the terrestrial carbon cycle, and to upscaled NEE estimates from FLUXCOM. Statistically significant correlations (P<0.05) are obtained between posterior NEE anomalies and anomalies in soil temperature and FLUXCOM NEE on continental and larger scales in the tropics, as well as in the northern extratropics on subcontinental scales during the summer (R2≥0.49), suggesting that GOSAT measurements provide a constraint on NEE interannual variability (IAV) on these spatial scales. Furthermore, we show that GOSAT flux inversions are generally better correlated with the environmental proxies and FLUXCOM NEE than NEE anomalies produced by a set of terrestrial biosphere models (TBMs), suggesting that GOSAT flux inversions could be used to evaluate TBM NEE fluxes.Environment and Climate Change CanadaNatural Sciences and Engineering Research Council of CanadaCanadian Space Agenc
TWEAK/Fn14 pathway modulates properties of a human microvascular endothelial cell model of blood brain barrier
Evidence for Restriction of Ancient Primate Gammaretroviruses by APOBEC3 but Not TRIM5α Proteins
Because of evolutionary pressures imposed through episodic colonization by retroviruses, many mammals express factors, such as TRIM5α and APOBEC3 proteins, that directly restrict retroviral replication. TRIM5 and APOBEC restriction factors are most often studied in the context of modern primate lentiviruses, but it is likely that ancient retroviruses imposed the selective pressure that is evident in primate TRIM5 and APOBEC3 genes. Moreover, these antiretroviral factors have been shown to act against a variety of retroviruses, including gammaretroviruses. Endogenous retroviruses can provide a ‘fossil record’ of extinct retroviruses and perhaps evidence of ancient TRIM5 and APOBEC3 antiviral activity. Here, we investigate whether TRIM5 and APOBEC3 proteins restricted the replication of two groups of gammaretroviruses that were endogenized in the past few million years. These endogenous retroviruses appear quite widespread in the genomes of old world primates but failed to colonize the human germline. Our analyses suggest that TRIM5α proteins did not pose a major barrier to the cross-species transmission of these two families of gammaretroviruses, and did not contribute to their extinction. However, we uncovered extensive evidence for inactivation of ancient gammaretroviruses through the action of APOBEC3 cytidine deaminases. Interestingly, the identities of the cytidine deaminases responsible for inactivation appear to have varied in both a virus and host species–dependent manner. Overall, sequence analyses and reconstitution of ancient retroviruses from remnants that have been preserved in the genomes of modern organisms offer the opportunity to probe and potentially explain the evolutionary history of host defenses against retroviruses
Gene Expression Profiles of the NCI-60 Human Tumor Cell Lines Define Molecular Interaction Networks Governing Cell Migration Processes
Although there is extensive information on gene expression and molecular interactions in various cell types, integrating those data in a functionally coherent manner remains challenging. This study explores the premise that genes whose expression at the mRNA level is correlated over diverse cell lines are likely to function together in a network of molecular interactions. We previously derived expression-correlated gene clusters from the database of the NCI-60 human tumor cell lines and associated each cluster with function categories of the Gene Ontology (GO) database. From a cluster rich in genes associated with GO categories related to cell migration, we extracted 15 genes that were highly cross-correlated; prominent among them were RRAS, AXL, ADAM9, FN14, and integrin-beta1. We then used those 15 genes as bait to identify other correlated genes in the NCI-60 database. A survey of current literature disclosed, not only that many of the expression-correlated genes engaged in molecular interactions related to migration, invasion, and metastasis, but that highly cross-correlated subsets of those genes engaged in specific cell migration processes. We assembled this information in molecular interaction maps (MIMs) that depict networks governing 3 cell migration processes: degradation of extracellular matrix, production of transient focal complexes at the leading edge of the cell, and retraction of the rear part of the cell. Also depicted are interactions controlling the release and effects of calcium ions, which may regulate migration in a spaciotemporal manner in the cell. The MIMs and associated text comprise a detailed and integrated summary of what is currently known or surmised about the role of the expression cross-correlated genes in molecular networks governing those processes
Wandering spleen with gastric volvulus and intestinal non-rotation in an adult male patient
Stratospheric and mesospheric data assimilation: the role of middle atmospheric dynamics
The middle atmosphere refers to the stratosphere and mesosphere and features dynamics and circulationsthat are fundamentally different from those of the troposphere. The large-scale meridional circulations inthe middle atmosphere operate on seasonal and longer time scales and are largely forced by the breakingof upward propagating waves. The winter stratosphere is dominated by large-scale waves and a polarvortex which confines constituents and which is sometimes punctuated by stratospheric sudden warmings.In contrast, the summer stratosphere is quiescent. Meanwhile, the meridional circulation in themesosphere is mainly driven by the breaking of a broad spectrum of gravity waves that have propagatedupward from the troposphere. These facets of middle atmosphere dynamics have implications for, andpose unique challenges to, data assimilation systems whose models encompass this region of theatmosphere. In this work, we provide an overview of middle atmosphere data assimilation in the contextof the dynamics of this region. The purpose is to demonstrate how the dynamics can be used to explainthe behavior of data assimilation systems in the middle atmosphere, and also to identify challenges inassimilating measurements from this region of the atmosphere. There are two overarching themes.Firstly, we consider the vertical propagation of information through waves, resolved and parameterized,and background error covariances. Secondly, we delve into the dynamical sources of model errors andtechniques for their estimation.Fil: Polavarapu, Saroja. Environment and Climate Change Canada; CanadáFil: Pulido, Manuel Arturo. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura. Departamento de FÃsica; Argentina. Consejo Nacional de Investigaciones CientÃficas y Técnicas. Centro CientÃfico Tecnológico Conicet - Nordeste. Instituto de Modelado e Innovación Tecnológica. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Modelado e Innovación Tecnológica; Argentin