34 research outputs found
Chickpea
The narrow genetic base of cultivated chickpea warrants systematic collection,
documentation and evaluation of chickpea germplasm and particularly wild
Cicer species for effective and efficient use in chickpea breeding programmes.
Limiting factors to crop production, possible solutions and ways to overcome
them, importance of wild relatives and barriers to alien gene introgression and
strategies to overcome them and traits for base broadening have been discussed.
It has been clearly demonstrated that resistance to major biotic and abiotic
stresses can be successfully introgressed from the primary gene pool
comprising progenitor species. However, many desirable traits including high
degree of resistance to multiple stresses that are present in the species
belonging to secondary and tertiary gene pools can also be introgressed by
using special techniques to overcome pre- and post-fertilization barriers.
Besides resistance to various biotic and abiotic stresses, the yield QTLs have
also been introgressed from wild Cicer species to cultivated varieties. Status
and importance of molecular markers, genome mapping and genomic tools
for chickpea improvement are elaborated. Because of major genes for various
biotic and abiotic stresses, the transfer of agronomically important traits into
elite cultivars has been made easy and practical through marker-assisted
selection and marker-assisted backcross. The usefulness of molecular markers
such as SSR and SNP for the construction of high-density genetic maps of
chickpea and for the identification of genes/QTLs for stress resistance, quality
and yield contributing traits has also been discussed
Cerium oxide nanoparticles promote neurogenesis and abrogate hypoxia-induced memory impairment through AMPK–PKC–CBP signaling cascade
Aditya Arya,1 Anamika Gangwar,1 Sushil Kumar Singh,2 Manas Roy,3,4 Mainak Das,3 Niroj Kumar Sethy,1 Kalpana Bhargava1 1Peptide and Proteomics Division, Defense Institute of Physiology and Allied Sciences, 2Functional Materials Division, Solid State Physics Laboratory, Defense Research and Development Organization, Timarpur, Delhi, 3Biological Science and Bioengineering, Indian Institute of Technology, Kanpur, 4Department of Chemistry, Indian Institute of Engineering Science and Technology, Howrah, India Abstract: Structural and functional integrity of the brain is adversely affected by reduced oxygen saturation, especially during chronic hypoxia exposure and often encountered by altitude travelers or dwellers. Hypoxia-induced generation of reactive nitrogen and oxygen species reportedly affects the cortex and hippocampus regions of the brain, promoting memory impairment and cognitive dysfunction. Cerium oxide nanoparticles (CNPs), also known as nanoceria, switch between +3 and +4 oxidation states and reportedly scavenge superoxide anions, hydrogen peroxide, and peroxynitrite in vivo. In the present study, we evaluated the neuroprotective as well as the cognition-enhancing activities of nanoceria during hypobaric hypoxia. Using polyethylene glycol-coated 3 nm nanoceria (PEG-CNPs), we have demonstrated efficient localization of PEG-CNPs in rodent brain. This resulted in significant reduction of oxidative stress and associated damage during hypoxia exposure. Morris water maze-based memory function tests revealed that PEG-CNPs ameliorated hypoxia-induced memory impairment. Using microscopic, flow cytometric, and histological studies, we also provide evidences that PEG-CNPs augmented hippocampus neuronal survival and promoted neurogenesis. Molecular studies revealed that PEG-CNPs promoted neurogenesis through the 5'-adenine monophosphate-activated protein kinase–protein kinase C–cyclic adenosine monophosphate response element-binding protein binding (AMPK-PKC-CBP) protein pathway. Our present study results suggest that nanoceria can be translated as promising therapeutic molecules for neurodegenerative diseases. Keywords: cerium oxide nanoparticles, oxidative stress, memory, hypoxia, neuroprotectio