Determination of tetracycline and its major degradation products by liquid chromatography with fluorescence detection

A liquid chromatographic method of tetracycline and its major degradation products on a C8-reversed phase column with acidic mobile phase and fluorescence detection is described. The quantification limit, measured as the amount of sample that gave a signal ten times the peak-to-peak noise of the baseline, was: 0.25 ng for tetracycline (TC) and epitetracycline (ETC), 25 ng for and 4-epianhydrotetracycline (EATC) and 50 ng for anhydrotetracycline (ATC) of injected standard. By means of this liquid chromatography (LC) assay TC, ETC, EATC and ATC as main degradation products of tetracycline, can be separated and determined with good sensitivity and specificity within 15 min.http://www.sciencedirect.com/science/article/B6TGX-43HVHWC-1D/1/9763379e028400de01242a673bd4528

Selection of sugarcane families and parents by Reml/Blup.

Were evaluated 113 full-sib families obtained by unbalanced diallel matings in three experiments in an augmented block design (ABD) planted side by side in the same area. The individual and joint analyses of the experiments were performed by the Reml/Blup method. The use of the ABD without replication did not prove adequate in experiments of family selection owing to the low heritability estimate at the level of family means in comparison to the joint analysis of the three experiments. The results presented predominance of the additive effects for all evaluated traits: number of stalks, tons of stalks per hectare and mean stalk weight. The components of estimated means via Blup allowed the selection of families and superior parents

CHO cell adhesion on modified surfaces of different materials.

Epithelial cells are mainly responsible for the formation of tissues that cover the external and internal surfaces of organs like skin, lining of the lungs and intestines. The cells must adhere to substrates and to each other in compliance with certain stimulus. In this way, adhesion properties can be regulated by the cell which simultaneously senses the chemical and mechanical properties of its environment. Their adhesion and growth on biomaterials depends on substrate properties such as surface wettability, topography and chemistry. The aim of this study is to investigate cell-surface interactions using several materials and different surfaces

Unidades piloto do sistema de produção integrada de feijão comum (Phaseolus vulgaris L.) em plantios irrigados.

O Projeto de Produção Integrada de Feijão Comum pertence ao Sistema Agropecuário de Produção Integrada (SAPI), do Ministério da Agricultura, Pecuária e Abastecimento (MAPA). É coordenado pela Embrapa Arroz e Feijão, em parceria com universidades, empresas públicas e privadas de assistência técnica e extensão rural, indústrias (beneficiamento e processamento), cooperativas e produtores. A implantação das Unidades Piloto tem como objetivo comparar os sistemas de produção integrada e convencional de feijão comum, analisando-se quanto ao impacto ambiental e aos aspectos socioeconômico e financeiro

Surface modification by metal ion implantation forming metallic nanoparticles in insulating matrix.

There is special interest in the incorporation of metallic nanoparticles in a surrounding dielectric matrix for obtaining composites with desirable characteristics such as for surface plasmon resonance, which can be used in photonics and sensing, and controlled surface electrical conductivity. We investigated nanocomposites produced through metallic ion implantation in insulating substrate, where the implanted metal self-assembles into nanoparticles. During the implantation, the excess of metal atom concentration above the solubility limit leads to nucleation and growth of metal nanoparticles, driven by the temperature and temperature gradients within the implanted sample including the beam-induced thermal characteristics. The nanoparticles nucleate near the maximum of the implantation depth profile (projected range), that can be estimated by computer simulation using the TRIDYN. This is a Monte Carlo simulation program based on the TRIM (Transport and Range of Ions in Matter) code that takes into account compositional changes in the substrate due to two factors: previously implanted dopant atoms, and sputtering of the substrate surface. Our study suggests that the nanoparticles form a bidimentional array buried few nanometers below the substrate surface. More specifically we have studied Au/PMMA (polymethylmethacrylate), Pt/PMMA, Ti/alumina and Au/alumina systems. Transmission electron microscopy of the implanted samples showed the metallic nanoparticles formed in the insulating matrix. The nanocomposites were characterized by measuring the resistivity of the composite layer as function of the dose implanted. These experimental results were compared with a model based on percolation theory, in which electron transport through the composite is explained by conduction through a random resistor network formed by the metallic nanoparticles. Excellent agreement was found between the experimental results and the predictions of the theory. It was possible to conclude, in all cases, that the conductivity process is due only to percolation (when the conducting elements are in geometric contact) and that the contribution from tunneling conduction is negligible

Evaluation of scientifi c production, patents and human resources training in the Brazilian nursing Avaliação da produção científi ca, patentes e formação de recursos humanos da Enfermagem Brasileira Evaluación de la producción científi ca, patentes y la

ABSTRACT Objective: to evaluate scientifi c production, patents and human resources training of nursing researchers with scholarships of research productivity (PQ) in National Counsel for Technological and Scientifi c Development (CNPq) in the 2000-2012 historic series; to verify the association between this production and characteristics of the researchers regarding gender, education and origin. Methods: this analytic cross-sectional study whose inclusion criterion was to be a nursing PQ/CNPq researcher during the period in question. We analyzed curriculum lattes of 208 researchers with scholarships between 2000 and 2012. For statistical analyses we used the SPSS ® software. Results: the study points to female predominance, concentration in the Southeast region and existence of an association between scientifi c production, patents and human resources training and education, gender and origin of the researcher. Conclusion: the study presents a signifi cant participation of nursing PQ/CNPq researchers in scientifi c production and in human resources training, and a modest involvement in the production of patents. Key words: Scientifi c Production Indicators; Researchers; Nursing. RESUMO Objetivo: avaliar produção científi ca, patentes e formação de recursos humanos de pesquisadores da enfermagem com bolsas de produtividade em pesquisa (PQ) no CNPq na série histórica 2000-2012; verifi car a associação entre esta produção e características dos pesquisadores quanto a sexo, formação e origem. Método: estudo transversal analítico cujo critério de inclusão foi ser pesquisador PQ/CNPq da enfermagem no período em questão. Foram analisados curriculum lattes de 208 pesquisadores com bolsas entre 2000-2012. Para as análises estatísticas utilizou-se o programa SPSS ® . Resultados: o estudo aponta predominância do sexo feminino, concentração na região Sudeste e existência de associação entre produção científi ca, patentes e formação de recursos humanos e a formação, sexo e origem do pesquisador. Conclusão: o estudo revela uma expressiva participação dos pesquisadores PQ/CNPq da enfermagem na produção científi ca e na formação de recursos humanos e tímida participação na produção de patentes. Descritores: Indicadores de Produção Científi ca; Pesquisadores; Enfermagem. RESUMEN Objetivo: evaluar la producción científi ca, patentes y formación de recursos humanos de enfermería investigadores con becas de productividad en investigación (PQ) en el CNPq en la serie temporal 2000-2012 y la existencia de una asociación entre estos tipos de producción y las características de los investigadores sexo, educación y origen. Método: estudio transversal cuyo criterio de inclusión fue ser un PQ investigador/enfermería CNPq en el período en cuestión

Hydrogen peroxide production regulates the mitochondrial function in insulin resistant muscle cells: Effect of catalase overexpression

AbstractThe mitochondrial redox state plays a central role in the link between mitochondrial overloading and insulin resistance. However, the mechanism by which the ROS induce insulin resistance in skeletal muscle cells is not completely understood. We examined the association between mitochondrial function and H2O2 production in insulin resistant cells. Our hypothesis is that the low mitochondrial oxygen consumption leads to elevated ROS production by a mechanism associated with reduced PGC1α transcription and low content of phosphorylated CREB. The cells were transfected with either the encoded sequence for catalase overexpression or the specific siRNA for catalase inhibition. After transfection, myotubes were incubated with palmitic acid (500μM) and the insulin response, as well as mitochondrial function and fatty acid metabolism, was determined. The low mitochondrial oxygen consumption led to elevated ROS production by a mechanism associated with β-oxidation of fatty acids. Rotenone was observed to reduce the ratio of ROS production. The elevated H2O2 production markedly decreased the PGC1α transcription, an effect that was accompanied by a reduced phosphorylation of Akt and CREB. The catalase transfection prevented the reduction in the phosphorylated level of Akt and upregulated the levels of phosphorylated CREB. The mitochondrial function was elevated and H2O2 production reduced, thus increasing the insulin sensitivity. The catalase overexpression improved mitochondrial respiration protecting the cells from fatty acid-induced, insulin resistance. This effect indicates that control of hydrogen peroxide production regulates the mitochondrial respiration preventing the insulin resistance in skeletal muscle cells by a mechanism associated with CREB phosphorylation and β-oxidation of fatty acids

Validação do sistema de produção integrada do feijão comum (Phaseolus vulgaris L.) na região central brasileira.

Com o objetivo de comparar os sistemas de Produção Integrada e convencional de feijão, foram implementadas cinco Unidades Piloto, em Cristalina-GO e Unaí-MG, municípios que se destacam no cenário nacional na produção do feijão. Nas Unidades Piloto, as tecnologias propostas para a PI foram comparadas, lado a lado, com a produção convencional, realizada pelo produtor.bitstream/item/31796/1/circ87.pd