Chemical Composition And Biological Activity Of Natural Extracts Obtained From A Brazilian Aromatic Plant (aloysia Gratissima) By Supercritical Co 2 And Hydrodistillation

Aloysia gratissima (Gillies & Hook.) Tronc. (Verbenaceae) is an aromatic shrub native to South America which is widely used in traditional medicine to treat several diseases. In this study we report on the chemical composition and biological activity (anti-inflammatory and antioxidant activity) of extracts obtained from the aerial parts of the Brazilian plant Aloysia gratissima using two extraction methods: hydrodistillation and extraction with supercritical carbon dioxide. Three major components were identified in the essential oil, namely trans-pinocamphone (13.5-16.3%), β-pinene (10.5-12.0%) and α-guaiol (6.6-8.7%), whereas the major compounds were guaiol (16.7%), β-caryophyllene (9.2%) and germacrene D (8.6%) in the supercritical fluid (SFE) extract. None of the two samples presented a significant inhibitory effect in vitro on soybean 5-lipoxygenase.The antioxidant activity using both the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ORAC assays showed poor activity. © Essential Oil Resource Consultants. All rights reserved.14172178Duarte, M.C.T., Figueira, G.M., Sartoratto, A., Rehder, V.L.G., Delarmelina, C., Anti-candida activity of Brazilian medicinal plants (2005) J Ethnopharmacol, 97 (2), pp. 305-311Alves, T.M.A., Silva, A.F., Brandão, M., Grandi, T.S.M., Smânia, E.F.A., Smânia Jr, A., Zani, C.L., Biological screening of Brazilian medicinal plants (2000) Mem Inst Oswaldo Cruz, 95 (3), pp. 367-373De Figueiredo RO, Stefanini MB, Ming LC, Marques MOM, Facanali R. Essential oil composition of Aloysia triphylla (L'Herit) Britton leaves cultivated in Botucatu, Sao Paulo, Brazil.Acta Hort. 2004;629:131-4Da Silva, C.C., Vandresen, F., de Oliveira, C.M.A., Kato, L., Tanaka, C.M.A., Ferreira, H.D., Chemical composition of Aloysia gratissima (Gill et Hook) Tronc. (Verbenaceae) (2006) Biochem Sys Ecol, 34, pp. 593-595Ricciardi, G.A.L., van Baren, C.M., Di Leo Lira, P., Ricciardi, A.I.A., Lorenzo, D., Dellacassa, E., Bandoni, A.L., Volatile constituents from aerial parts of Aloysia gratissima (Gillies & Hook.) Tronc. van gratissima growing in Corrientes, Argentina (2006) Flavour Fragr J, 21 (4), pp. 698-703Bassols, G., Gurni, A., Especies del género Lippia utilizadas en medicina popular latino americana. (1996) Dominguezia, 13 (1), pp. 7-25Lorenzi, H., Matos, F.J.A., Plantas medicinais no Brasil: Nativas e exoticas cultivadas (2002) Instituto Plantarum, p. 512De Riscala, E.C., Talenti, E.C.J., Retamar, J.A., Essential oil of Aloysia lycioides (1973) Essenze, Derivati Agrumari, 43 (3), pp. 291-298Bravo, A.H., Aiquel, G.R., Retamar, J.A., Essential oils of the province of Tucuman. Essence of Aloysia gratissima (1974) Int Congress Essent Oil 6thToursarkissian M. Plantas medicinales de la Argentina. Ed. Hemisferio Sur SA, Bs.As.1980Zygadlo, J.A., Lamarque, A.L., Guzman, C.A., Composition of the flower oils of some Lippia and Aloysia species from Argentina (1995) J Essent Oil Res, 7, pp. 593-595Bailac, P.N., Dellacasa, A.D., Duschatzky, C.B., Firpo, N., Ponzi, M.I., (1999) Composition of the essential oil Aloysia gratissima from San Luis, 87 (3-4), pp. 149-153. , Argentina. Anales de la Asociacion Quimica ArgentinaDellacasa, A.D., Bailac, P.N., Ponzi, M.I., In vitro activity of essential oils from San Luis-Argentina against Ascosphaera apis (2003) J Essent Oil Res, 15, pp. 282-285Garcia, C.C., Talarico, L., Almeida, N., Colombres, S., Duschatsky, C., Damonte, E.B., Virucidal Activity of Essential oils from Aromatic Plants of San Luis, Argentina (2003) Phytother Res, 17, pp. 1073-1075Duschatzky, C.B., Martinez, A.N., Almeida, N.V., Bonivardo, L.S., Nematicidal activity of the essential oils of several Argentina Plants against the root-knot nematode (2004) J Essent Oil Res, 16, pp. 626-628Soler, E., Dellacasa, E., Moyna, P., Composition of Aloysia gratissima flower essential oil (1986) Planta Medica, 6, pp. 488-490Soler, E., Dellacasa, E., Moyna, P., Composition of Aloysia gratissima leaf essential oil (1986) Phytochem, 25 (6), pp. 1343-1345Bauer L, Brasil A, Silva GA de. Sobre o óleo essencial de Lippia lycioides Steud. (Verbenaceae). Tribuna Farmacêutica. 1969;37(2):151-9Trovati, G., Sanchez, E.A., Galhiane, M.S., Chierice, G.O., (2003) Proceeding of the II Simpósio Brasileiro de Óleos Essenciais, p. 122. , CampinasMontanha, J.A., Moellerke, P., Bordignon, S.A.L., Schenkel, E.P., Roehe, P.M., Antiradical activity of Brazilian plant extracts (2004) Acta Farmaceutica Bonaerense, 23 (2), pp. 183-186Romero, A.R., Saavedra, G., Screening Bolivian Plants for Antioxidant Activity (2005) Pharmaceut Biol, 43 (1), pp. 79-86Demirbas, A., Supercritical fluid extraction and chemicals from biomass with supercritical fluids (2001) Energy Convers. Manage, 42 (3), pp. 279-294Kakasy, A.Z., Lemberkovics, E., Simándi, B., Lelik, E., Héthelyi, E., Antal, I., Szöke, E., Comparative study of traditional essential oil and Supercritical fluid extracts of Moldavian dragonhead (Dracocephalum moldavica) (2006) Flav Fragr J, 21 (4), pp. 598-603França, L.F., Reber, G., Meireles, M.A.A., Machado, N.T., Brunner, G., Supercritical extraction of carotenoids and lipids from Buriti (Mauritia flexuosa), a fruit from the Amazon region (1999) J Supercrit Fluids, 14 (3), pp. 247-256Wang, H.C., Chen, C.R., Chang, C.J., Carbon dioxide extraction of ginseng root hair oil and ginsenosides (2001) Food Chem, 72 (4), pp. 505-509Yoda, S.K., Marques, M.O.M., Petenate, A.J., Meireles, M.A.A., Supercritical fluid extraction from Stevia rebaudiana Bertoni using CO2 and CO 2+water: Extraction kinetics and identification of extracted components (2003) J Food Eng, 57, pp. 125-143Zetzl, C., Brunner, G., Meireles, M.A.A., Standardised low-cost barch SFE-units dor University Education and Comparative Research (2003) Proceedings of the 6th International Symposium on Supercritical Fluids, , Versailles FranceAdams, R.P., (2001) Identification of Essential Oils by Gas Chromatography Quadrupole Mass Spectroscopy, , Carol Stream, USA:Allured Publishing Corporation;Joulain, D., König, W.A., (1998) The Atlas of Spectral Data of Sesquiterpene hydrocarbons, , Hamburg, Germany: EB Verlag;König, W.A., Joulain, D., Hochmuth, D.H., (2004) Terpenoids and related constituents of essential oils. Mass Finder 3, , www.massfmder.comAbad, M.J., Bermejo, P., Villar, A., The activity of flavonoids extracted from Tanacetum microphyllum DC. Compositae on soybean lipoxygenase and prostaglandin synthetase (1995) Gen Pharmacol, 26 (4), pp. 815-819Braga, F.C., Wagner, H., Lomberdi, J.A., de Oliveira, A.B., Screening Brazilian plant species for in vitro inhibition of 5-lipoxygenase (1999) Phytoméd, 6, pp. 447-452Safayhi, H., Mack, T., Sabiaeraj, J., Anazodo, M.I., Subramanian, L.R., Ammon, H.P.T., Boswelic acids: Novel, specific, non-redox inhibitors of 5-lipoxygenase (1992) J Pharmacol Exper Ther, 261 (3), pp. 1143-1146Sircar, J.C., Schwender, C.F., Johnson, E.A., Soybean lipoxygenase inhibition by non-steroidal anti-inflammatory drugs (1983) Prostaglandins, 25 (3), pp. 393-396Mellors, A., Tappel, A.L., The inhibition of mitochondrial peroxidation by ubiquinone and ubiquinol (1966) J Biol Chem, 241 (19), pp. 4353-4356Agnaniet, H., Menut, C., Bessière, J.M., Aromatic plants of Tropical Central Africa. Part XLIX: Chemical composition of essential oils of the leaf and rhizome of Aframomum giganteum K. Schum from Gabon (2004) Flav Fragr J, 19, pp. 205-209Gil, E.S., Serrano, S.H.P., Soares, L.A., Rezende, K.R., Atividade antioxidante de extrato etanólico e hidroalcoólico de "Canjiqueira" (Byrsonima orbygniana) dosamento de rutina, quercetina, ácido elagio e ácido ascórbico (2005) Rev Eletrônica Farmácia, 2 (2), pp. 85-88Ou, B., Hampsch-Woodill, M., Prior, R.L., Development and validation of an improved Oxygen Radical Absorbance Capacity assay using Fluorescein as the Fluorescent probe (2001) J Agric Food Chem, 49, pp. 4619-4626Huang, D., Ou, B., Hampsch-Woodill, M., Flanagan, J.A., Deemer, E.K., Development and validation of Oxygen Radical Absorbance Capacity assay for lipophilic antioxidants using randomly methylated β-cyclodextrin as the solubility enhancer (2002) J Agric Food Chem, 50, pp. 1815-1821Ou, B., Hampsch-Woodill, M., Flanagan, J.A., Deemer, E.K., Prior, R.L., Huang, D., Novel fluorimetric assay for hydroxyl radical prevention capacity using fluorescein as the probe (2002) J Agric Food Chem, 50, pp. 2772-2777Arctander, S., (1960) Perfume and Flavor Materials of Natural Origins, , Elisabeth, NJ USA:Allured Publishing;Steinmetz, M.D., Tognetti, P., Mourgue, M., Jouglard, J., Millet, Y., Toxicity of certain commercial essential oils: Oil of hyssop and oil of sage (1980) Plantes Médicinales Phytothér, 14 (1), pp. 34-45Alitonou, G.A., Avlessi, F., Sohounhloue, D.K., Agnaniet, H., Bessière, J.M., Menut, C., Investigations on the essential oil of Cymbopogon giganteus from Benin for its potential use as an anti-inflammatory agent (2006) Int J Aromather, 16, pp. 37-4