50,272 research outputs found

    Retrotransposons represent the most labile fraction for genomic rearrangements in polyploid plant species

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    Understanding how increased genome size and diversity within polyploid genomes impacts plant evolution and breeding continues to be challenging. Although historical studies by McClintock suggested the importance of transposable elements mediated by polyploidisation on genomic changes, data from plant crosses remain scarce. Despite the absence of a conclusive proof regarding autonomous retrotransposon movement in synthetic allopolyploids, the transposition of retrotransposons and their ubiquitous dispersion in all plant species might explain the positive correlation between the genome size of plants and the prevalence of retrotransposons. Here, we address polyploidisationmediated rearrangements of retrotransposon-associated sequences and discuss a tendency for a preferential restructuring of large ancestral genomes after polyploidisation. A comparative analysis of the frequency of modifications of retrotransposon-associated sequences in synthetic polyploids with marked differences in genome sizes is presented. Such analyses suggest the absence of a significant difference in the rates of rearrangements despite vast dissimilarities in the retrotransposon copy number between species, which emphasises the high plasticity of this genomic feature. See also the sister article focusing on animals by Arkhipova and Rodriguez in this themed issu

    Unravelling genome dynamics in Arabidopsis synthetic auto and allopolyploid species

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    Polyploidization is a major genome modification that results in plant species with multiple chromosome sets. Parental genome adjustment to co-habit a new nuclear environment results in additional innovation outcomes. We intended to assess genomic changes in polyploid model species with small genomes using inter retrotransposons amplified polymorphism (IRAP) and retrotransposon microsatellite amplified polymorphism (REMAP). Comparative analysis among diploid and autotetraploid A. thaliana and A. suecica lines with their parental lines revealed a marginal fraction of novel bands in both polyploids, and a vast loss of parental bands in allopolyploids. Sequence analysis of some remodelled bands shows that A. suecica parental band losses resulted mainly from sequence changes restricted to primer domains. Moreover, in A. suecica, both parental genomes presented rearrangement frequencies proportional to their sizes. Overall rates of genomic remodelling events detected in A. suecica were similar to those observed in species with a large genome supporting the role of retrotransposons and microsatellite sequences in the evolution of most allopolyploidsAcknowledgements: M. Bento was funded by a FCT (Fundação para a Ciência e a Tecnologia, Portugal) postdoctoral grant (SFRH/BPD/80550/2011), Diana Tomás was funded by a FCT doctoral scholarship (SFRH/BD/93156/2013), Manuela Silva by the FCT Investigator Programme (IF/00834/2014), and the research work was financed by FCT LEAF Unit (UID/AGR/04129/2013)

    On the Levi-Civita solutions with cosmological constant

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    The main properties of the Levi-Civita solutions with the cosmological constant are studied. In particular, it is found that some of the solutions need to be extended beyond certain hypersurfaces in order to have geodesically complete spacetimes. Some extensions are considered and found to give rise to black hole structure but with plane symmetry. All the spacetimes that are not geodesically complete are Petrov type D, while in general the spacetimes are Petrov type I.Comment: Typed in Revtex, including two figures. To appear in Phys. Rev.

    Differential effects of high-temperature stress on nuclear topology and transcription of repetitive noncoding and coding rye sequences

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    The plant stress response has been extensively characterized at the biochemical and physiological levels. However, knowledge concerning repetitive sequence genome fraction modulation during extreme temperature conditions is scarce. We studied high-temperature effects on subtelomeric repetitive sequences (pSc200) and 45S rDNA in rye seedlings submitted to 40 ° C during 4 h. Chromatin organization patterns were evaluated through fluorescent in situ hybridization and transcription levels were assessed using quantitative real-time PCR. Additionally, the nucleolar dynamics were evaluated through fibrillarin immunodetection in interphase nuclei. The results obtained clearly demonstrated that the pSc200 sequence organization is not affected by high-temperature stress (HTS) and proved for the first time that this noncoding subtelomeric sequence is stably transcribed. Conversely, it was demonstrated that HTS treatment induces marked rDNA chromatin decondensation along with nucleolar enlargement and a significant increase in ribosomal gene transcription. The role of noncoding and coding repetitive rye sequences in the plant stress response that are suggested by their clearly distinct behaviors is discussed. While the hetero-chromatic conformation of pSc200 sequences seems to be involved in the stabilization of the interphase chromatin architecture under stress conditions, the dynamic modulation of nucleolar and rDNA topology and transcription suggest their role in plant stress response pathway

    Variational Density Matrix Method for Warm Condensed Matter and Application to Dense Hydrogen

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    A new variational principle for optimizing thermal density matrices is introduced. As a first application, the variational many body density matrix is written as a determinant of one body density matrices, which are approximated by Gaussians with the mean, width and amplitude as variational parameters. The method is illustrated for the particle in an external field problem, the hydrogen molecule and dense hydrogen where the molecular, the dissociated and the plasma regime are described. Structural and thermodynamic properties (energy, equation of state and shock Hugoniot) are presented.Comment: 26 pages, 13 figures. submitted to Phys. Rev. E, October 199

    On parameters of the Levi-Civita solution

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    The Levi-Civita (LC) solution is matched to a cylindrical shell of an anisotropic fluid. The fluid satisfies the energy conditions when the mass parameter σ\sigma is in the range 0σ10 \le \sigma \le 1. The mass per unit length of the shell is given explicitly in terms of σ\sigma, which has a finite maximum. The relevance of the results to the non-existence of horizons in the LC solution and to gauge cosmic strings is pointed out.Comment: Latex, no figure

    The Equation of State and the Hugoniot of Laser Shock-Compressed Deuterium

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    The equation of state and the shock Hugoniot of deuterium are calculated using a first-principles approach, for the conditions of the recent shock experiments. We use density functional theory within a classical mapping of the quantum fluids [ Phys. Rev. Letters, {\bf 84}, 959 (2000) ]. The calculated Hugoniot is close to the Path-Integral Monte Carlo (PIMC) result. We also consider the {\it quasi-equilibrium} two-temperature case where the Deuterons are hotter than the electrons; the resulting quasi-equilibrium Hugoniot mimics the laser-shock data. The increased compressibility arises from hot D+eD^+-e pairs occuring close to the zero of the electron chemical potential.Comment: Four pages; One Revtex manuscript, two postscipt figures; submitted to PR

    Emergence of Participatory Rural Appraisal (PRA) Technique as a Strategy towards Sustainable Development: A Sri Lankan Experience

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    In this millennium all the development activities are mostly focused on sustainable development, i.e. the development which fulfils the requirements of the present without disturbing the utilization of future generation. Basically, the sustainable development deals with environmental, social, and economical initiations. In relation to these three objectives, community participation plays a key role as an effective strategy for sustainable development. Among the numerous types of participation, Participatory Rural Appraisal (PRA) technique is the most relevant effective method to receive the participation. Because, it has been strengthen by bottom up approach, well defined objectives, practicable solutions, and remedies. Hence, the out come of such an event is most productive rather than a top bottom approach techniques. In fact, a PRA was practiced to develop a strategic plan for tsunami affected village – Bambaranda east, in southern province of Sri Lanka. PRA sessions were carried out during February, 2007 by the Department of Agric. Economics of Ruhuna University, Sri Lanka in collaboration with Japanese Green Resource Agency, Japan.Participatory mapping, venn diagram, matrix ranking, preference ranking, and pair - wise ranking were demonstrated to gather information from the community. The tsunami affected area, including the paddy fields, four irrigation canals were shown by the group with the help of the participatory map. Preference ranking was resulted the reconstruction of irrigation canals as the most important rehabilitation activity to recover the livelihood of villagers. Intrusion of sea water into the paddy fields was the main limitation revealed by the pair - wise ranking. The second limitation marked as unavailability of enough fertilizer and the dilapidated irrigation canals was the third that has to be solved. Matrix ranking was employed to identify the most facilitated sectors by the government and other institutes in order to detect the areas which need to pay further attention. By that, the community realized that the rehabilitation of irrigation canal is the least benefited area where any development activity should be addressed in order to up grade their livelihood. Finally, a venn diagram was executed to identify the service providing entities in the community. It explored the Agrarian Service Center (ASC) as the most important service providing institute which keeps a close relationship with the community. The second and third places were acquired by Sanasa and Samurdhi office. Therefore, the most appropriate institute to launch any sort of development activity is ASC, Sanasa, or Samurdhi office.With the light of these exercises, now the funding agency is activating in Bambaranda village by constructing the irrigation canals and also the estuary. Moreover, the implementation was carried out under the supervision of ASC with efficient participation of villagers. In fact, the PRA has given the correct path that would direct towards sustainable development with community participation

    Combining type I and type II seesaw mechanisms in the minimal 3-3-1 model

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    The minimal 3-3-1 model is perturbative until energies around 4-5TeV, posing a challenge to generate neutrino masses at eV scale, mainly if one aims to take advantage of the seesaw mechanism. As a means to circumvent this problem we propose a modification of the model such that it accommodates the type I and type II seesaw mechanisms altogether. We show that the conjunction of both mechanisms yield a neutrino mass expression suppressed by a high power of the cutoff scale, M5M^5, in its denominator. With such a suppression term we naturally obtain neutrino masses at eV scale when MM is around few TeV. We also investigate the size of lepton flavor violation through the process μeγ\mu \rightarrow e\gamma.Comment: about 15 pages, no figure
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