1-(4-{[(E)-3-Eth­oxy-2-hy­droxy­benzyl­idene]amino}­phen­yl)ethanone oxime

In the title compound, C17H18N2O3, the benzene rings form a dihedral angle of 3.34 (2)°. There is a strong intra­molecular O—H⋯N hydrogen bonds (which induces planarity of the structure). In the crystal, mol­ecules are linked by pairs of O—H⋯N hydrogen bonds, forming inversion dimers

A UPLCâ MS/MS method for simultaneous determination of five flavonoids from Stellera chamaejasme L. in rat plasma and its application to a pharmacokinetic study

Stellera chamaejasme L. has been used as a traditional Chinese medicine for the treatment of scabies, tinea, stubborn skin ulcers, chronic tracheitis, cancer and tuberculosis. A sensitive and selective ultraâ high liquid chromatographyâ tandem mass spectrometry (UPLCâ MS/MS) method was developed and validated for the simultaneous determination of five flavonoids (stelleranol, chamaechromone, neochamaejasmin A, chamaejasmine and isochamaejasmin) of S. chamaejasme L. in rat plasma. Chromatographic separation was accomplished on an Agilent Poroshell 120 ECâ C18 column (2.1 à 100â mm, 2.7â μm) with gradient elution at a flow rate of 0.4â mL/min and the total analysis time was 7â min. The analytes were detected using multiple reaction monitoring in positive ionization mode. The samples were prepared by liquidâ liquid extraction with ethyl acetate. The UPLCâ MS/MS method was validated for specificity, linearity, sensitivity, accuracy and precision, recovery, matrix effect and stability. The validated method exhibited good linearity (r â ¥ 0.9956), and the lower limits of quantification ranged from 0.51 to 0.64â ng/mL for five flavonoids. The intraâ and interâ day precision were both <10.2%, and the accuracy ranged from â 11.79 to 9.21%. This method was successfully applied to a pharmacokinetic study of five flavonoids in rats after oral administration of ethyl acetate extract of S. chamaejasme L.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/144258/1/bmc4189.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/144258/2/bmc4189_am.pd

Mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) in Chinese patients with congenital bilateral absence of vas deferens

AbstractBackgroundGenetic testing of the cystic fibrosis transmembrane conductance (CFTR) gene is currently performed in patients with congenital bilateral absence of vas deferens (CBAVD). This study was conducted to investigate the role of mutations in the CFTR gene in CBAVD-dependent male infertility.Methods73 Chinese patients diagnosed with CBAVD were studied. The entire coding regions and splice sites of 27 exons of the CFTR gene were sequenced in 146 chromosomes from the 73 CBAVD patients. Screening was carried out using PCR, gel electrophoresis and DNA sequencing to identify novel variants of the entire coding regions and boundaries of the 27 exons.ResultsFive novel nonsynonymous mutations, three novel splice site mutations and one deletion were identified by sequencing. Apart from the novel variants, we also found 19 previously reported mutations and polymorphism sites. Thirty-four patients (46.57%) had the 5T variant (6 homozygous and 28 heterozygous) and in two of them it was not associated with any detectable mutation of the CFTR gene. All potential pathogenic mutations are not contained in the 1000 Genome Project database. In total, the present study identified 30 potential pathogenic variations in the CFTR gene, 9 of which had not previously been described.ConclusionsMost patients with CBAVD have mutations in the CFTR gene. A mild genotype with one or two mild or variable mutations was observed in all the patients. These findings improve our understanding of the distribution of CFTR alleles in CBAVD patients and will facilitate the development of more sensitive CFTR mutation screening

Metal-Organic Frameworks with Double Channels for Rapid and Reversible Adsorption of 1,2-Ethylenediamine and Gases.

Selective liquid and gas adsorptions are important for environmental control and industrial processes. Here, unique porous lanthanide-organic frameworks of [Ln2(1,3-pdta)2(H2O)2] n 2 n - {Ln = La ( 1 ), Ce ( 2 ), Pr ( 3 ), and Nd ( 4 ), 1,3-pdta = CH2[CH2N(CH2CO2H)2]2} are template-synthesized by 1,2-ethylenediamine and fully characterized, which possess hydrophobic and hydrophilic open channels simultaneously. The skeletons are stable up to 200 °C. Obvious downfield shifts have been observed for 1,2-ethylenediamine in the confined channel with solid-state 13C NMR measurement. The ammonium salt is directly used for the removal of 1,2-ethylenediamine in water. Its saturated adsorption capacity is reached in <1 min and can be regenerated easily with a similar uptake capacity. Moreover, the materials can also selectively adsorb O2, CH4, and CO2, respectively, which is useful for CO2/CH4, CO2/H2, and O2/N2 separation. The combined hydrophobic and hydrophilic open channels of the lanthanides make them promising functional materials for the elimination of 1,2-ethylenediamine and gas separations