22 research outputs found
A comparison of methods for purification and concentration of norovirus GII-4 capsid virus-like particles
Get PDFNoroviruses (NoVs) are one of the leading causes of acute gastroenteritis worldwide. NoV GII-4 VP1 protein was expressed in a recombinant baculovirus system using Sf9 insect cells. Several methods for purification and concentration of virus-like particles (VLPs) were evaluated. Electron microscopy (EM) and histo-blood group antigen (HBGA) binding assays showed that repeated sucrose gradient purification followed by ultrafiltration resulted in intact VLPs with excellent binding to H type 3 antigens. VLPs were stable for at least 12 months at 4°C, and up to 7 days at ambient temperature. These findings indicate that this method yielded stable and high-quality VLPs
Effects of Emerita brasiliensis flour supplementation on normotensive (Wistar) and spontaneously hypertensive rats (SHR)
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Bioprocessing of plant-derived virus-like particles of Norwalk virus capsid protein under current Good Manufacture Practice regulations
No full textLow Pathogenic Avian Influenza Viruses (H3N8, H5N6): In Vitro Influence of d,l-Lactic Acid and Sodium Chloride on Infectivity and Virus Persistence in Short Fermented Raw Poultry Sausage
No full textGeneration of recombinant MVA-norovirus: a comparison study of bacterial artificial chromosome- and marker-based systems
No full textAntifungal activity of low molecular weight chitosan against clinical isolates of Candida
No full textA Refinement of an International Standard Method (ISO/TS 15216–2:2013) to Allow Extraction and Concentration of Human Enteric Viruses from Tissues of Edible Snail Species
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