9 research outputs found
Insertion of a “chloroplast-like” regulatory segment responsible for thiol modulation into γ-subunit of F0F1-ATPase of the cyanobacterium Synechocystis 6803 by mutagenesis of atpC
No full textMultiple Genes in a Single Host: Cost-Effective Production of Bacterial Laccase (cotA), Pectate Lyase (pel), and Endoxylanase (xyl) by Simultaneous Expression and Cloning in Single Vector in E. coli
No full textGlobal metabolic rewiring for improved CO2 fixation and chemical production in cyanobacteria
No full textCyanobacteria have attracted much attention as hosts to recycle CO(2) into valuable chemicals. Although cyanobacteria have been engineered to produce various compounds, production efficiencies are too low for commercialization. Here we engineer the carbon metabolism of Synechococcus elongatus PCC 7942 to improve glucose utilization, enhance CO(2) fixation and increase chemical production. We introduce modifications in glycolytic pathways and the Calvin Benson cycle to increase carbon flux and redirect it towards carbon fixation. The engineered strain efficiently uses both CO(2) and glucose, and produces 12.6 g l(−1) of 2,3-butanediol with a rate of 1.1 g l(−1) d(−1) under continuous light conditions. Removal of native regulation enables carbon fixation and 2,3-butanediol production in the absence of light. This represents a significant step towards industrial viability and an excellent example of carbon metabolism plasticity
Physical and gene maps of the unicellular cyanobacterium Synechococcus sp. strain PCC6301 genome
No full textStatistical Screening of Medium Components for Recombinant Production of Pseudomonas aeruginosa ATCC 9027 Rhamnolipids by Nonpathogenic Cell Factory Pseudomonas putida KT2440
No full text
