Murine vaginal colonization model for investigating asymptomatic mucosal carriage of streptococcus pyogenes

While many virulence factors promoting Streptococcus pyogenes invasive disease have been described, specific streptococcal factors and host properties influencing asymptomatic mucosal carriage remain uncertain. To address the need for a refined model of prolonged S. pyogenes asymptomatic mucosal colonization, we have adapted a preestrogenized murine vaginal colonization model for S. pyogenes. In this model, derivatives of strains HSC5, SF370, JRS4, NZ131, and MEW123 established a reproducible, asymptomatic colonization of the vaginal mucosa over a period of typically 3 to 4 weeks' duration at a relatively high colonization efficiency. Prior treatment with estradiol prolonged streptococcal colonization and was associated with reduced inflammation in the colonized vaginal epithelium as well as a decreased leukocyte presence in vaginal fluid compared to the levels of inflammation and leukocyte presence in non-estradiol-treated control mice. The utility of our model for investigating S. pyogenes factors contributing to mucosal carriage was verified, as a mutant with a mutation in the transcriptional regulator catabolite control protein A (CcpA) demonstrated significant impairment in vaginal colonization. An assessment of in vivo transcriptional activity in the CcpA(−) strain for several known CcpA-regulated genes identified significantly elevated transcription of lactate oxidase (lctO) correlating with excessive generation of hydrogen peroxide to self-lethal levels. Deletion of lctO did not impair colonization, but deletion of lctO in a CcpA(−) strain prolonged carriage, exceeding even that of the wild-type strain. Thus, while LctO is not essential for vaginal colonization, its dysregulation is deleterious, highlighting the critical role of CcpA in promoting mucosal colonization. The vaginal colonization model should prove effective for future analyses of S. pyogenes mucosal colonization

Pyroelectric detectors

The multi-agency, long-term Global Change programs, and specifically NASA's Earth Observing system, will require some new and advanced photon detector technology which must be specifically tailored for long-term stability, broad spectral range, cooling constraints, and other parameters. Whereas MCT and GaAs alloy based photovoltaic detectors and detector arrays reach most impressive results to wavelengths as long as 12 microns when cooled to below 70 K, other materials, such as ferroelectrics and pyroelectrics, appear to offer special opportunities beyond 12 microns and above 70 K. These materials have found very broad use in a wide variety of room temperature applications. Little is known about these classes of materials at sub-room temperatures and no photon detector results have been reported. From the limited information available, researchers conclude that the room temperature values of D asterisk greater than or equal to 10(exp 9) cm Hz(exp 1/2)/W may be improved by one to two orders of magnitude upon cooling to temperatures around 70 K. Improvements of up to one order of magnitude appear feasible for temperatures achievable by passive cooling. The flat detector response over a wavelength range reaching from the visible to beyond 50 microns, which is an intrinsic advantage of bolometric devices, makes for easy calibration. The fact that these materials have been developed for reduced temperature applications makes ferro- and pyroelectric materials most attractive candidates for serious exploration

Multi-Line Gamma-Ray Spectrometer Performance of a Si(Li) Detector Stack

Experimental data is presented which for the first time displays multi-line spectrometer performance of a Si(Li) detector stack at elevated temperature. The stack consists of four elements, each with a 2 cm diameter active area. Ba-133 and Ag-110m spectra are obtained using various techniques to enhance the peak-to-background ratio. Spectral data are shown as a function of temperature (94 K less than or = T less than or = 230 K) using optimized peak shaping

The personal aircraft: Status and issues

Paper summarizes the status of personal air transportation with emphasis upon VTOL and converticar capability. The former obviates the need for airport operations for personal aircraft whereas the latter provides both ground and air capability in the same vehicle. Fully automatic operation, ATC and navigation is stressed along with consideration of acoustic, environmental and cost issues

Variable Temperature Performance of a Si(Li) Detector Stack

New experimental data is presented which displays 137Cs resolution of both single Si(Li) devices and a detector stack 2 cm in height as a function of temperature (85 K greater than or equal to T greater than or equal to 245 K). We also discuss variations in photopeak shape which indicate that detector charge collection may be temperature dependent over the range of interest

Discontinuous Galerkin method for the spherically reduced BSSN system with second-order operators

We present a high-order accurate discontinuous Galerkin method for evolving the spherically-reduced Baumgarte-Shapiro-Shibata-Nakamura (BSSN) system expressed in terms of second-order spatial operators. Our multi-domain method achieves global spectral accuracy and long-time stability on short computational domains. We discuss in detail both our scheme for the BSSN system and its implementation. After a theoretical and computational verification of the proposed scheme, we conclude with a brief discussion of issues likely to arise when one considers the full BSSN system.Comment: 35 pages, 6 figures, 1 table, uses revtex4. Revised in response to referee's repor

K2 Optical Emission from OJ 287 and Other Gamma-Ray Blazars on Hours-to-Weeks Timescales from 2014-2018

We present second observations by K2 of OJ~287 and 7 other $\gamma$-ray AGNs obtained in 2017-2018, second and third observations of the lobe-dominated, steep spectrum quasar 3C~207, and observations of 9 additional blazars not previously observed with K2. The AGN were observed simultaneously with K2 and the Fermi Large Area Telescope for 51-81 days. Our full sample, observed in 2014-2018, contained 16 BL Lac objects (BL Lacs), 9 Flat Spectrum Radio Quasars (FSRQs), and 4 other $\gamma$-ray AGNs. Twelve BL Lacs and 7 FSRQs exhibited fast, jagged light curves while 4 BL Lacs and 2 FSRQs had slow, smooth light curves. Some objects changed their red-noise character significantly between repeated K2 observations. The optical characteristics of OJ~287 derived from the short-cadence K2 light curves changed between observations made before and after the predicted passage of the suspected secondary supermassive black hole through the accretion disk of the primary supermassive black hole. The average slopes of the periodogram power spectral densities of the BL Lacs' and FSRQs' light curves differed significantly, by $\approx 12$\%, with the BL Lac slopes being steeper, and a KS test with a $p$-value of 0.039 indicates that these samples probably come from different populations; however, this result is not as strongly supported by PSRESP analyses. Differences in the origin of the jets from the ergosphere or accretion disk in these two classes could produce such a disparity, as could different sizes or locations of emission regions within the jets.Comment: 35 pages, 8 figures. Accepted for publication in the Astrophysical Journa