Usporedba dvaju inhibitora cikooksigenaze u eksperimentalnom modelu suhog oka u albino kunića

The purpose of this study was to compare the topical anti-inflammatory effects of the nonselective cyclooxygenase (COX) inhibitor, ketorolac, with the selective COX-2 inhibitor, nimesulide, in an animal model of dry eye in albino rabbits. All animals were examined by the Schirmer test, tear break-up time (TBUT) and fluorescein corneal staining test. Dry eye model showed significant reduction in tear volume, TBUT, corneal staining and histopathological signs of dryness and inflammation. On treating dry eye model with nimesulide 0.1% eye drops and ketorolac 0.5% eye drops, there were improvements in Schirmer test values, TBUT and fluorescein corneal staining and histopathologically reduced inflammatory reaction, with signs of healing and regeneration. Both nimesulide and ketorolac ameliorate atropine sulphate induced dry eye in albino rabbits. The use of selective COX-2 inhibitor, nimesulide, is preferred to avoid local and systemic side effects which may occur with the use of the nonselective COX inhibitor, ketorolac.Cilj istraživanja bila je usporedba topičkog protuupalnog učinka neselektivnog inhibitora ciklooksigenaze (COX), ketorolaka i selektivnog COX-2 inhibitora, nimesulida na animalnom modelu suhog oka u albino kunića. Na životinjama je proveden Schirmerov test, vrijeme prestanka suzenja oka tear break-up time (TBUT) i test bojenja rožnice fluoresceinom. U animalnom modelu suhog oka značajno je smanjen volumen suza, TBUT, bojenje rožnice i histopatološki simptomi suhoće i upale. Obradom suhog oka s kapima za oči koje sadrže 0,1% nimesulida, odnosno 0,5% ketorolaka, poboljšane su vrijednosti u Schirmerovom testu, TBUT i bojanje rožnice fluoresceinom, smanjena je upalna reakcija, a na oku su se pokazali simptomi ozdravljenja i regeneracije. I nimesulid i ketorolak smanjuju suhoću oka induciranu atropin sulfatom u albino kunića. Uporaba selektivnog COX-2 inhibitora, nimesulida, se preferira jer se izbjegavaju lokalne i sistemske nuspojave koje se mogu pojaviti uz uporabu neselektivnog COX inhibitora, ketorolaka

Novel Complex Unbalanced Dicentric X-Autosome Rearrangement in a Thoroughbred Mare with a Mild Effect on the Phenotype

Complex structural X chromosome abnormalities are rare in humans and animals, and not recurrent. Yet, each case provides a fascinating opportunity to evaluate X chromosome content and functional status in relation to the effect on the phenotype. Here, we report the first equine case of a complex unbalanced X-autosome rearrangement in a healthy but short in stature Thoroughbred mare. Studies of about 200 cells by chromosome banding and FISH revealed an abnormal 2n=63,X,der(X;16) karyotype with a large dicentric derivative chromosome (der). The der was comprised of normal Xp material, a palindromic duplication of Xq12q21, and a translocation of chromosome 16 to the inverted Xq12q21 segment by the centromere, whereas the distal Xq22q29 was deleted from the der. Microsatellite genotyping determined a paternal origin of the der. While there was no option to experimentally investigate the status of X chromosome inactivation (XCI), the observed mild phenotype of this case suggested the following scenario to retain an almost normal genetic balance: active normal X, inactivated X-portion of the der, but without XCI spreading into the translocated chromosome 16. Cases like this present unique resources to acquire information about species-specific features of X regulation and the role of X-linked genes in development, health, and disease