Chlorophyll a/b binding (CAB) polypeptides of CP29, the internal chlorophyll a/b complex of PSII: characterization of the tomato gene encoding the 26 kDa (type 1) polypeptide, and evidence for a second CP29 polypeptide

CP29, the core chlorophyll a/b (CAB) antenna complex of Photosystem II (PSII), has two nuclearencoded polypeptides of approximately 26 and 28 kDa in tomato ( Lycopersicon esculentum ). Cab9, the gene for the Type 1 (26 kDa) CP29 polypeptide was cloned by immunoscreening a tomato leaf cDNA library. Its identity was confirmed by sequencing tryptic peptides from the mature protein. Cab9 is a single-copy gene with five introns, the highest number found in a CAB protein. In vitro transcription-translation gave a 31 kDa precursor which was cleaved to about 26 kDa after import into isolated tomato chloroplasts. The Cab9 polypeptide has the two highly conserved regions common to all CAB polypeptides, which define the members of this extended gene family. Outside of the conserved regions, it is only slightly more closely related to other PSII CABs than to PSI CABs. Sequence analysis of tryptic peptides from the Type II (28 kDa) CP29 polypeptide showed that it is also a member of the CAB family and is very similar or identical to the CP29 polypeptide previously isolated from spinach. All members of the CAB family have absolutely conserved His, Gln and Asn residues which could ligate the Mg atoms of the chlorophylls, and a number of conserved Asp, Glu, Lys and Arg residues which could form H-bonds to the polar groups on the porphyrin rings. The two conserved regions comprise the first and third predicted trans-membrane helices and the stroma-exposed segments preceding them.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/47577/1/438_2004_Article_BF00259681.pd

The ECORS-Truc Vert’08 nearshore field experiment: Presentation of a three-dimensional morphologic system in a macro-tidal environment during consecutive extreme storm conditions

A large multi-institutional nearshore field experiment was conducted at Truc Vert, on the Atlantic coast of France in early 2008. Truc Vert’08 was designed to measure beach change on a long, sandy stretch of coast without engineering works with emphasis on large winter waves (offshore significant wave height up to 8 m), a three-dimensional morphology, and macro-tidal conditions. Nearshore wave transformation, circulation and bathymetric changes involve coupled processes at many spatial and temporal scales thus implying the need to improve our knowledge for the full spectrum of scales to achieve a comprehensive view of the natural system. This experiment is unique when compared with existing experiments because of the simultaneous investigation of processes at different scales, both spatially (from ripples to sand banks) and temporally (from single swash events to several spring-neap tidal cycles, including a major storm event). The purpose of this paper is to provide background information on the experiment by providing detailed presentation of the instrument layout and snapshots of preliminary results.Hydraulic EngineeringCivil Engineering and Geoscience