A spliced latency-associated VZV transcript maps antisense to the viral transactivator gene

Varicella-zoster virus (VZV), an alphaherpesvirus, establishes lifelong latent infection in the neurons of >90% humans worldwide, reactivating in one-third to cause shingles, debilitating pain and stroke. How VZV maintains latency remains unclear. Here, using ultra-deep virus-enriched RNA sequencing of latently infected human trigeminal ganglia (TG), we demonstrate the consistent expression of a spliced VZV mRNA, antisense to VZV open reading frame 61 (ORF61). The spliced VZV latency-associated transcript (VLT) is expressed in human TG neurons and encodes a protein with late kinetics in productively infected cells in vitro and in shingles skin lesions. Whereas multiple alternatively spliced VLT isoforms (VLTly) are expressed during lytic infection, a single unique VLT isoform, which specifically suppresses ORF61 gene expression in co-transfected cells, predominates in latently VZV-infected human TG. The discovery of VLT links VZV with the other better characterized human and animal neurotropic alphaherpesviruses and provides insights into VZV latency

OPTOCHEMICAL HCL-GAS DETECTION BASED ON ZINC TETRABROMOTETRAPHENYLPORPHYRIN DISPERSED IN ETHYL CELLULOSE

Four zinc tetrabromotetraphenylporphyrin dispersed in a polymer matrix, the absorbance at 479 nm and 722 nm is sensitive to sub-ppm levels of HCl gas and also good reversibility is observed at room temperature

OPTOCHEMICAL HCL GAS-DETECTION BASED ON TETRAPHENYLPORPHINE DISPERSED IN ETHYL CELLULOSE

To detect sub-ppm levels of dry HCl gas, spectral changes of tetraphenylporphine dispersed in ethylcellulose were examined in the visible region. The Soret and Q-bands of free base tetraphenylporphine are sensitive to sub-ppm levels of HCl with a good reversibility and insensitive to NH3, Cl-2, NO2 and NO at room temperature

OPTOCHEMICAL HCL-GAS DETECTION BASED ON ZINC TETRABROMOTETRAPHENYLPORPHYRIN DISPERSED IN ETHYL CELLULOSE

Four zinc tetrabromotetraphenylporphyrin dispersed in a polymer matrix, the absorbance at 479 nm and 722 nm is sensitive to sub-ppm levels of HCl gas and also good reversibility is observed at room temperature