Determination of Pitavastatin Calcium by Analytical Spectrophotometry

Simple and rapid spectrophotometric method for the quantitative analysis of Pitavastatin calcium (PTV) in raw material and tablets pharmaceutical formulation has been described. The method is based on the formation of yellow ion-pair complex between Pitavastatin calcium and Bromocresol purple (BCP) in chloroform medium. Different parameters affecting the reaction such as: effect of solvents, stability, reagent concentration, correlation ratio, etc. were optimized. The formed complex was quantified spectrophotometrically at absorption maximum 405 nm. Linearity range was 2.20 - 35.23 µg/mL, regression analysis showed a good correlation coefficient R2 = 0.9991. The limit of detection (LOD) and limit of quantification (LOQ) were to be 0.367 µg/mL and 1.112 µg/mL respectively. The average percent recovery was found to be (100.62 – 101.14) % for Pitavastatin Calcium. This study was applied on Syrian pharmaceutical trademark: (PAVACRIUM 4 & Londalop). The method was successfully applied for the determination of Pitavastatin calcium in tablets pharmaceutical formulation. The proposed method is simple, direct, sensitive and do not require any extraction process. Thus, this method could be readily applicable for the quality control and routine analysis

SIMULTANEOUS DETERMINATION OF TARTRAZINE AND BRILLIANT BLUE IN FOODSTUFFS BY SPECTROPHOTOMETRIC METHOD

Objective: Objective of the study was to develop a simple spectrophotometric method for simultaneous determination of two colorants, Tartrazine (T) and Brilliant Blue (BB), in foodstuffs without any prior treatment.Methods: Derivative spectrophotometric (DS) and spectrophotometric methods were applied for the determination of (T) and (BB), respectively.Results: Tartrazine was determined by first derivative spectrophotometry at 454 nm (1D454); where Brilliant blue was determined by zero-order spectrum at 630 nm. Linearity ranges were 2-24 ðœ‡g/ml for (T) and 2-14 ðœ‡g/ml for (BB), regression analysis showed a good correlation co-efficient R2=0.9999 and R2=0.9998 for (T) and (BB), respectively. The limit of detection (LOD) and limit of quantification (LOQ) was to be 0.12 and 0.35 ðœ‡g/ml for (T), 0.17 and 0.52 ðœ‡g/ml for (BB), respectively.Conclusion: The proposed methods were successfully applied to analysis individual or mixture of Tartrazine and Brilliant Blue in foodstuffs. All studied samples showed dye levels conformity with Syrian legislation. Â

DETERMINATION OF WATER-SOLUBLE VITAMINS B1, B2, B3, B6, B9, B12 AND C ON C18 COLUMN WITH PARTICLE SIZE 3 µM IN SOME MANUFACTURED FOOD PRODUCTS BY HPLC WITH UV-DAD/FLD DETECTION

Objective: Objective of the study was to develop a simple, precise and accurate RP-HPLC ion-pair method, for the determination of water-soluble vitamins (B1, B2, B3, B6, B9, B12, and C) in some manufactured food products. Methods: RP-HPLC with C18 BDS (100 x 4.6 mm; 3 µm) column were used. Mobile phase constituents were solvent (A): 5.84 mM of hexane-1-sulfonic acid sodium: acetonitrile (95:5) with 0.1% triethylamine at pH 2.5 and solvent (B): 5.84 mM of hexane-1-sulfonic acid sodium: acetonitrile (50:50) with 0.1% triethylamine at pH 2.5, flow rate 1.6 ml/min, at column temperature 40 °C and suitable detection wavelength. Results: Two different detectors were used: photo diode array detector (UV-DAD) and the fluorescence detector (FLD). Calibration graphs plotted with five concentrations of each vitamin where linear regression coefficients R2˃ 0.9972. LOQ values were 50.0, 81.0, 19.1, 19.0, 30.0, 9.7, 50.0 µg/l with DAD for vitamins B1, B2, B3, B6, B9, B12 and C respectively, and 5.7, 4.1 µg/l with FLD for vitamins B2, B6 respectively. LOD values were 16.5, 26.7, 6.3, 6.3, 9.9, 3.2, 16.5 µg/l with DAD for vitamins B1, B2, B3, B6, B9, B12 and C respectively, and 1.9, 1.3 µg/l with FLD for vitamins B2, B6 respectively. Conclusion: The proposed method was successfully applied to analysis mixture of seven water-soluble vitamins in pure form and in manufactured food products, with average recovery of 98.14% to 100.96%

Determination of Glibenclamide By Analytical Spectrophotometry

Simple and rapid spectrophotometric method was developed and applied to determine Glibenclamide (GB) by zero spectrophotometric method and first derivative spectrophotometric method for determining of (GB) in the presence of Metformin hydrochloride (MET). Zero spectrophotometric (ZS) method was applied for the determination of (GB) at λmax = 300 nm. Linearity range was (4 – 360) μg/mL. Regression analysis showed a good correlation coefficients R2 = 0.99993. The limit of detection (LOD) and limit of quantification (LOQ) were to be 0.65 μg/mL and 2.31 μg/mL, respectively.  First derivative spectrophotometric (1DS) method was applied for the determination of (GB) in the presence (MET). (GB) was determined at 317 nm (1D317). Linearity ranges were (4 – 240) μg/mL for (GB). Regression analysis showed a good correlation coefficients R2 = 0.999914. The limit of detection (LOD) and limit of quantification (LOQ) were to be 0.60 μg/mL and 1.83 μg/mL for (GB). The proposed zero spectrophotometry method was applied to analysis individual (GB), and the derivative (1D317) method was applied to analysis (GB) individually or combined with (MET) in Syrian trademark drugs. The proposed method is simple, direct, sensitive and do not require any extraction process. Thus, this method could be readily applicable for the quality control and routine analysis

Analytical Spectrometric Study For Determining Dapagliflozin Propanediol Monohydrate Individually Or In Presence Of Metformin Hydrochloride In Tablets Formulation

First simple spectrophotometric method was developed and applied to determine Dapagliflozin Propanediol  Monohydrate by Zero Spectrophotometry and First Derivative Spectrophotometric method for determining of Dapagliflozin Propanediol Monohydrate (DAPA) in the presence of Metformin Hydrochloride (MET). Zero spectrophotometric (ZS) was applied for the determination of (DAPA) at 223.5 nm. Linearity range was (2.61– 31.23) µg/mL. Regression analysis showed a good correlation coefficients R2 = 0.9989. The limit of detection (LOD) and limit of quantification (LOQ) were to be 0.569 µg/mL and 1.724 µg/mL, respectively. Derivative spectrophotometric (1DS) was applied for the determination of (DAPA) in the presence (MET). (DAPA) was determined at 233 nm (1D233).  Linearity ranges were (5.21 – 41.64) µg/mL for (DAPA). Regression analysis showed a good correlation coefficients R2 = 0.9994. The limit of detection (LOD) and limit of quantification (LOQ) were to be 0.732 µg/mL and 2.218 µg/mL for (DAPA). The proposed Zero spectrophotometry method was applied to analysis individual (DAPA), and the derivative (1D233) method was applied to analysis (DAPA) individually or with (MET) combination in Syrian trademark drugs

Simultaneous Determination Of Atenolol And Hydrocholrothiazide In Tablets Formulation By Derivative Spectrometry

The derivative spectrophotometric method was developed and applied for the simultaneous determination of Atenolol (ATE) and Hydrochlorothiazide (HCT) in Tablets formulations. The first derivative spectrophotometric (1DS) method was applied for the determination of (ATE) and (HCT), respectively. (ATE) was determined at 271.9 nm (1D 271.9) and (HCT) was determined at 279.3 nm (1D 279.3). Linearity showed a good correlation coefficients R2 = 0.9994 and R2 = 0.9989 for (ATE) and (HCT), respectively. Linearity ranges were (10 – 280

تأثير التطعيم الكيميائي في البنية السطحية للبنتونيت المسامي كعامل في الكروماتوغرافيا

The effect of thermal and chemical treatments on the variation of specific surface area of porous bentonite as a support was studied. The surface area of bare bentonite thermally treated at 950°C and then washed with 6N HC1 was measured to be 3.01 m^/g. Chemical grafting for the same treated bentonite by the condensation of PEG-20 M at 280° C formed a non-extractable monomolecular layer of PEG-20M. This grafting process changed the surface structure of bare bentonite and contributed to the good separation of hydrocarbon mixture C5-C12 with a very good precision and sensitivity.في هذا البحث تم دراسة تأثير تكرار المعالجة الكيميائية والحرارية على تغير المساحة السطحية النوعية للبنتونيت حيث وجد أن مساحة سطح البنتونيت المعالج عند 950ْ ثم بحمض ECL تبلغ 3.01 م2/جرا م. كما تم دراسة تأثير التطعيم الكيميائي للبنتونيت (المعالج حرارياً وكيميائياً ، وفقاً لما ذكر أعلاه ) بالبولي اثيلين جليكول M 20 (PEG – 20 M)على بنيته السطحية ، حيث أصبحت البنية السطحية له هي بنية البولي ايثيلين جليكول 20M وقد ساهم هذا مساهمة فعالة في التمكن من فصل مزائج الفحوم الهيدروجينية اللاقطبية من C5 إلى C12 بشكل أكثر دقة وحساسية من استخدام البنتونيت العاري أو المشروب بالبولي ايثيلين جليكول 20M