26 research outputs found

    Spleen_B-1a_TLR2x4dKO_1

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    Tlr2-/-Tlr4-/- C57BL/6 (Mus musculus) Female (#1) 7wk old Spleen B-1a cells: IgH repertoire analysis by iRepertoire Inc

    87691_pep

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    WT C57BL/6 (Mus musculus) Female (#2) 7wk old Spleen B-1a cells: IgH repertoire analysis by iRepertoire Inc

    Spleen_B-1a_Unc3d_2

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    Unc93b(3d/3d) C57BL/6 (Mus musculus) Female (#2) 7wk old Spleen B-1a cells: IgH repertoire analysis by iRepertoire Inc

    Spleen_B-1a_TLR2x4dKO_2

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    Tlr2-/-Tlr4-/- C57BL/6 (Mus musculus) Female (#2) 7wk old Spleen B-1a cells: IgH repertoire analysis by iRepertoire Inc

    PerC_B-1a_TLRKO_1

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    Tlr2-/-Tlr4-/-Unc93b13d/3d C57BL/6 (Mus musculus) Female (#1) 7wk old Peritoneal cavity B-1a cells: IgH repertoire analysis by iRepertoire Inc

    PerC_B-1a_TLR2x4dKO_1

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    Tlr2-/-Tlr4-/- C57BL/6 (Mus musculus) Female (#1) 7wk old Peritoneal cavity B-1a cells: IgH repertoire analysis by iRepertoire Inc

    PerC_B-1a_TLRKO_3

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    Tlr2-/-Tlr4-/-Unc93b13d/3d C57BL/6 (Mus musculus) Female (#3) 7wk old Peritoneal Cavity B-1a cells: IgH repertoire analysis by iRepertoire Inc

    Data from: B cell receptor and Toll-like receptor signaling coordinate to control distinct B-1 responses to both self and the microbiota

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    B-1a cells play an important role in mediating tissue homeostasis and protection against infections. They are the main producers of spontaneously secreted serum antibodies predominately reactive to self antigens, like phosphatidylcholine (PtC), or to the intestinal microbiota. The mechanisms that regulate the B-1a immunoglobulin (Ig) repertoire and their antibody secretion remain poorly understood. Here, we use a novel reporter mouse to demonstrate that production of self- and microbiota-reactive antibodies is linked to BCR signaling in B-1a cells. Moreover, we show that Toll-like receptors (TLRs) are critical for shaping the Ig repertoire of B-1a cells as well as regulating their antibody production. Strikingly, we find that both the colonization of a microbiota as well as microbial-sensing TLRs are required for anti-microbiota B-1a responses, whereas nucleic-acid sensing TLRs are required for anti-PtC responses, demonstrating that linked activation of BCR and TLRs controls steady state B-1a responses to both self and microbiota-derived antigens

    Spleen_B-1a_TLRKO_1

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    Tlr2-/-Tlr4-/-Unc93b13d/3d C57BL/6 (Mus musculus) Female (#1) 7wk old Spleen B-1a cells: IgH repertoire analysis by iRepertoire Inc

    Spleen_B-1a_TLRKO_2

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    Tlr2-/-Tlr4-/-Unc93b13d/3d C57BL/6 (Mus musculus) Female (#2) 7wk old Spleen B-1a cells: IgH repertoire analysis by iRepertoire Inc
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