12 research outputs found

    A cyano analogue of boswellic acid induces crosstalk between p53/PUMA/Bax and telomerase that stages the human papillomavirus type 18 positive HeLa cells to apoptotic death

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    The p53 tumor suppressor pathway is disrupted by human papillomavirus (HPV) in over 90% of cervical cancers. HPV E6 protein promotes the degradation of p53 thereby inhibiting its stabilization and activation. This study demonstrates that treatment with a novel cyano derivative of 11-keto-;2-boswellic acid, i.e. butyl 2- cyano-3, 11-dioxours-1,12-dien-24-oate (BCDD) reduced the viral E6 mRNA expression and lead to the accumulation of transcriptionally active p53 in the nucleus of HPV18 HeLa cells following DNA damage.Western blot analysis showed that BCDD robustly up regulated time-dependent expression of p53/PUMA/p21 whereas it deprived cells essentially of p-AKT and NF-;AB cell survival signalling cascade. BCDD appeared to gear up PUMA activation through p53 pathway and that both p53 and p21 translocated heavily into the nucleus. Simultaneously, it inhibited anti-apoptotic Bcl-2, augumented Drp-1 expression, disrupted mitochondrial functions causing the activation of proapoptotic proteins and caspases activation. Additionally,BCDD inhibited telomerase expression that's likely to result in a marked reduction of the tumorigenic potential of high-grade cervical cancers. Consequently BCDD caused apoptotic death in cervical cancer cells as evidenced by DNA fragmentation and PARP-cleavage. Further, BCDD did not affect the extrinsic signalling transduction pathway as depicted by its null effect on caspase-8. The in vivo anticancer activity of BCDD was investigated in Ehrlich Ascites carcinoma model where it exhibited tumor regression by 48% at 30 mg/kg, i.p.,in mice. These findings indicated that BCDD is a potential candidate that may be found useful in the management of cervical cancer

    EMS-induced cytomictic variability in safflower (Carthamus tinctorius L.)

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    Seeds of safflower (Carthamus tinctorius L.) were subjected to three treatment durations (3h, 5h and 7h) of 0.5 % Ethyl Methane Sulphonate (EMS). Microsporogenesis was carried out in the control as well as in the treated materials. EMS treated plants showed interesting feature of partial inter-meiocyte chromatin migration through channel formation, beak formation or direct cell fusion. Another interesting feature noticed during the study was the fusion among tetrads due to wall dissolution. The phenomenon of cytomixis was recorded at nearly all the stages of microsporogenesis connecting from a few to several meiocytes. Other abnormalities such as laggards, precocious movement, bridge and non disjunction of chromosomes were also recorded but in very low frequencies. The phenomenon of cytomixis increased along with the increase in treatment duration of EMS. Cells with these types of cytomictic disturbances may probably result in uneven formation of gametes or zygote, heterogenous sized pollen grains or even loss of fertility in future.Π‘Π΅ΠΌΠ΅Π½Π° сафлора (Carthamus tinctorius L.) ΠΎΠ±Ρ€Π°Π±Π°Ρ‚Ρ‹Π²Π°Π»ΠΈ 0.5%-Π½Ρ‹ΠΌ ΡΡ‚ΠΈΠ»ΠΌΠ΅Ρ‚Π°Π½ΡΡƒΠ»ΡŒΡ„ΠΎΠ½Π°Ρ‚ΠΎΠΌ (ЭМБ) Π² ΠΎΠ΄Π½ΠΎΠΌ ΠΈΠ· Ρ‚Ρ€Π΅Ρ… Ρ€Π΅ΠΆΠΈΠΌΠΎΠ² – 3, 5 ΠΈ 7 Ρ‡. ΠœΠΈΠΊΡ€ΠΎΡΠΏΠΎΡ€ΠΎΠ³Π΅Π½Π΅Π· ΠΈΠ·ΡƒΡ‡Π°Π»ΠΈ ΠΊΠ°ΠΊ Π² ΠΊΠΎΠ½Ρ‚Ρ€ΠΎΠ»Π΅, Ρ‚Π°ΠΊ ΠΈ Π² ΠΎΠ±Ρ€Π°Π±ΠΎΡ‚Π°Π½Π½ΠΎΠΌ ΠΌΠ°Ρ‚Π΅Ρ€ΠΈΠ°Π»Π΅. РастСния, ΠΎΠ±Ρ€Π°Π±ΠΎΡ‚Π°Π½Π½Ρ‹Π΅ ЭМБ, проявляли ΠΈΠ½Ρ‚Π΅Ρ€Π΅ΡΠ½ΡƒΡŽ ΠΎΡΠΎΠ±Π΅Π½Π½ΠΎΡΡ‚ΡŒ частичной ΠΈΠ½Ρ‚Π΅Ρ€ΠΌΠ΅ΠΉΠΎΡ†ΠΈΡ‚Π½ΠΎΠΉ ΠΌΠΈΠ³Ρ€Π°Ρ†ΠΈΠΈ Ρ…Ρ€ΠΎΠΌΠ°Ρ‚ΠΈΠ½Π° ΠΏΡ€ΠΈ Ρ„ΠΎΡ€ΠΌΠΈΡ€ΠΎΠ²Π°Π½ΠΈΠΈ ΠΊΠ°Π½Π°Π»ΠΎΠ², ΠΎΠ±Ρ€Π°Π·ΠΎΠ²Π°Π½ΠΈΠΈ ΠΊΠ»ΡŽΠ²ΠΎΠΎΠ±Ρ€Π°Π·Π½ΠΎΠ³ΠΎ выступа ΠΈΠ»ΠΈ прямом слиянии ΠΊΠ»Π΅Ρ‚ΠΎΠΊ. Π”Ρ€ΡƒΠ³ΠΈΠΌ ΠΎΠ±Π½Π°Ρ€ΡƒΠΆΠ΅Π½Π½Ρ‹ΠΌ Π² исслСдовании явлСниСм Π±Ρ‹Π»ΠΎ слияниС Ρ‚Π΅Ρ‚Ρ€Π°Π΄ ΠΈΠ·-Π·Π° растворСния стСнки. Π€Π΅Π½ΠΎΠΌΠ΅Π½ цитомиксиса отмСчался ΠΏΠΎΡ‡Ρ‚ΠΈ Π½Π° всСх стадиях микроспорогСнСза ΠΈ Π·Π°Ρ‚Ρ€Π°Π³ΠΈΠ²Π°Π» ΠΎΡ‚ Π½Π΅ΡΠΊΠΎΠ»ΡŒΠΊΠΈΡ… Π΄ΠΎ ΠΌΠ½ΠΎΠ³ΠΈΡ… ΠΌΠ΅ΠΉΠΎΡ†ΠΈΡ‚ΠΎΠ². Π”Ρ€ΡƒΠ³ΠΈΠ΅ Π°Π½ΠΎΠΌΠ°Π»ΠΈΠΈ, Ρ‚Π°ΠΊΠΈΠ΅ ΠΊΠ°ΠΊ отставания, ΠΏΡ€Π΅ΠΆΠ΄Π΅Π²Ρ€Π΅ΠΌΠ΅Π½Π½Ρ‹Π΅ двиТСния, мосты ΠΈ нСраздСлСния хромосом, ΠΎΡ‚ΠΌΠ΅Ρ‡Π°Π»ΠΈΡΡŒ с Π½Π΅Π·Π½Π°Ρ‡ΠΈΡ‚Π΅Π»ΡŒΠ½ΠΎΠΉ частотой. Π€Π΅Π½ΠΎΠΌΠ΅Π½ цитомиксиса возрастал с ΡƒΠ²Π΅Π»ΠΈΡ‡Π΅Π½ΠΈΠ΅ΠΌ Π΄Π»ΠΈΡ‚Π΅Π»ΡŒΠ½ΠΎΡΡ‚ΠΈ ΠΎΠ±Ρ€Π°Π±ΠΎΡ‚ΠΊΠΈ ЭМБ. ΠšΠ»Π΅Ρ‚ΠΊΠΈ с этими Ρ‚ΠΈΠΏΠ°ΠΌΠΈ цитомиктичСских Π½Π°Ρ€ΡƒΡˆΠ΅Π½ΠΈΠΉ ΠΌΠΎΠ³ΡƒΡ‚, вСроятно, ΠΏΡ€ΠΈΠ²ΠΎΠ΄ΠΈΡ‚ΡŒ ΠΊ нСрСгулярному Ρ„ΠΎΡ€ΠΌΠΈΡ€ΠΎΠ²Π°Π½ΠΈΡŽ Π³Π°ΠΌΠ΅Ρ‚ ΠΈΠ»ΠΈ Π·ΠΈΠ³ΠΎΡ‚, Π³Π΅Ρ‚Π΅Ρ€ΠΎΠ³Π΅Π½Π½Ρ‹Ρ… ΠΏΠΎ Ρ€Π°Π·ΠΌΠ΅Ρ€Ρƒ ΠΏΡ‹Π»ΡŒΡ†Π΅Π²Ρ‹Ρ… Π·Π΅Ρ€Π΅Π½, ΠΈΠ»ΠΈ Π΄Π°ΠΆΠ΅ ΠΊ ΠΏΠΎΡ‚Π΅Ρ€Π΅ Ρ„Π΅Ρ€Ρ‚ΠΈΠ»ΡŒΠ½ΠΎΡΡ‚ΠΈ Π² Π±ΡƒΠ΄ΡƒΡ‰Π΅ΠΌ.Насіння сафлору (Carthamus tinctorius L.) обробляли 0.5%-Π½ΠΈΠΌ Π΅Ρ‚ΠΈΠ»ΠΌΠ΅Ρ‚Π°Π½ΡΡƒΠ»ΡŒΡ„ΠΎΠ½Π°Ρ‚ΠΎΠΌ (Π•ΠœΠ‘) Π² ΠΎΠ΄Π½ΠΎΠΌΡƒ Π· Ρ‚Ρ€ΡŒΠΎΡ… Ρ€Π΅ΠΆΠΈΠΌΡ–Π² – 3, 5 Ρ– 7 Π³ΠΎΠ΄. ΠœΡ–ΠΊΡ€ΠΎΡΠΏΠΎΡ€ΠΎΠ³Π΅Π½Π΅Π· Π²ΠΈΠ²Ρ‡Π°Π»ΠΈ як Ρƒ ΠΊΠΎΠ½Ρ‚Ρ€ΠΎΠ»Ρ–, Ρ‚Π°ΠΊ Ρ– Π² ΠΎΠ±Ρ€ΠΎΠ±Π»Π΅Π½ΠΎΠΌΡƒ ΠΌΠ°Ρ‚Π΅Ρ€Ρ–Π°Π»Ρ–. Рослини, ΠΎΠ±Ρ€ΠΎΠ±Π»Π΅Π½Ρ– Π•ΠœΠ‘, виявляли Ρ†Ρ–ΠΊΠ°Π²Ρƒ ΠΎΡΠΎΠ±Π»ΠΈΠ²Ρ–ΡΡ‚ΡŒ часткової Ρ–Π½Ρ‚Π΅Ρ€ΠΌΠ΅ΠΉΠΎΡ†ΠΈΡ‚Π½ΠΎΡ— ΠΌΡ–Π³Ρ€Π°Ρ†Ρ–Ρ— Ρ…Ρ€ΠΎΠΌΠ°Ρ‚ΠΈΠ½Ρƒ ΠΏΡ€ΠΈ Ρ„ΠΎΡ€ΠΌΡƒΠ²Π°Π½Π½Ρ– ΠΊΠ°Π½Π°Π»Ρ–Π², ΡƒΡ‚Π²ΠΎΡ€Π΅Π½Π½Ρ– Π΄Π·ΡŽΠ±ΠΎΠΏΠΎΠ΄Ρ–Π±Π½ΠΎΠ³ΠΎ Ρ‡ΠΈ прямому Π·Π»ΠΈΡ‚Ρ‚Ρ– ΠΊΠ»Ρ–Ρ‚ΠΈΠ½. Π†Π½ΡˆΠΈΠΌ виявлСним Π² дослідТСнні явищСм Π±ΡƒΠ»ΠΎ злиття Ρ‚Π΅Ρ‚Ρ€Π°Π΄ Ρ‡Π΅Ρ€Π΅Π· розчинСння стінки. Π€Π΅Π½ΠΎΠΌΠ΅Π½ Ρ†ΠΈΡ‚ΠΎ- міксису відзначався ΠΌΠ°ΠΉΠΆΠ΅ Π½Π° всіх стадіях ΠΌΡ–ΠΊΡ€ΠΎ-спорогСнСзу Ρ– Π·Π°Ρ‡Ρ–ΠΏΠ°Π² Π²Ρ–Π΄ ΠΊΡ–Π»ΡŒΠΊΠΎΡ… Π΄ΠΎ Π±Π°Π³Π°Ρ‚ΡŒΠΎΡ… ΠΌΠ΅ΠΉΠΎΡ†ΠΈΡ‚Ρ–Π². Π†Π½ΡˆΡ– Π°Π½ΠΎΠΌΠ°Π»Ρ–Ρ—, Ρ‚Π°ΠΊΡ– як відставання, пСрСдчасні Ρ€ΡƒΡ…ΠΈ, мости Ρ– нСрозділСння хромосом, Π·ΡƒΡΡ‚Ρ€Ρ–Ρ‡Π°Π»ΠΈΡΡŒ Π· Π½Π΅Π·Π½Π°Ρ‡- ною Ρ‡Π°ΡΡ‚ΠΎΡ‚ΠΎΡŽ. Π€Π΅Π½ΠΎΠΌΠ΅Π½ цитоміксису зростав Ρ–Π· Π·Π±Ρ–Π»ΡŒΡˆΠ΅Π½Π½ΡΠΌ тривалості ΠΎΠ±Ρ€ΠΎΠ±ΠΊΠΈ Π•ΠœΠ‘. ΠšΠ»Ρ–Ρ‚ΠΈΠ½ΠΈ Π· Ρ†ΠΈΠΌΠΈ Ρ‚ΠΈΠΏΠ°ΠΌΠΈ Ρ†ΠΈΡ‚ΠΎΠΌΡ–ΠΊΡ‚ΠΈΡ‡Π½ΠΈΡ… ΠΏΠΎΡ€ΡƒΡˆΠ΅Π½ΡŒ ΠΌΠΎΠΆΡƒΡ‚ΡŒ, ΠΉΠΌΠΎΠ²Ρ–Ρ€Π½ΠΎ, ΠΏΡ€ΠΈΠ²ΠΎΠ΄ΠΈΡ‚ΠΈ Π΄ΠΎ нСрСгулярного формування Π³Π°ΠΌΠ΅Ρ‚ Π°Π±ΠΎ Π·ΠΈΠ³ΠΎΡ‚, Π³Π΅Ρ‚Π΅Ρ€ΠΎΠ³Π΅Π½Π½ΠΈΡ… Π·Π° Ρ€ΠΎΠ·ΠΌΡ–Ρ€ΠΎΠΌ ΠΏΠΈΠ»ΠΊΠΎΠ²ΠΈΡ… Π·Π΅Ρ€Π΅Π½, Π°Π±ΠΎ Π½Π°Π²Ρ–Ρ‚ΡŒ Π΄ΠΎ Π²Ρ‚Ρ€Π°Ρ‚ΠΈ Ρ„Π΅Ρ€Ρ‚ΠΈΠ»ΡŒΠ½ΠΎΡΡ‚Ρ– Π² ΠΌΠ°ΠΉΠ±ΡƒΡ‚Π½ΡŒΠΎΠΌΡƒ
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