12 research outputs found
A cyano analogue of boswellic acid induces crosstalk between p53/PUMA/Bax and telomerase that stages the human papillomavirus type 18 positive HeLa cells to apoptotic death
The p53 tumor suppressor pathway is disrupted by human papillomavirus (HPV) in over 90% of cervical
cancers. HPV E6 protein promotes the degradation of p53 thereby inhibiting its stabilization and activation.
This study demonstrates that treatment with a novel cyano derivative of 11-keto-;2-boswellic acid, i.e. butyl 2-
cyano-3, 11-dioxours-1,12-dien-24-oate (BCDD) reduced the viral E6 mRNA expression and lead to the accumulation of transcriptionally active p53 in the nucleus of HPV18 HeLa cells following DNA damage.Western blot analysis showed that BCDD robustly up regulated time-dependent expression of p53/PUMA/p21 whereas it deprived cells essentially of p-AKT and NF-;AB cell survival signalling cascade. BCDD appeared to gear up PUMA activation through p53 pathway and that both p53 and p21 translocated heavily into the nucleus. Simultaneously, it inhibited anti-apoptotic Bcl-2, augumented Drp-1 expression, disrupted mitochondrial functions causing the activation of proapoptotic proteins and caspases activation. Additionally,BCDD inhibited telomerase expression that's likely to result in a marked reduction of the tumorigenic potential of high-grade cervical cancers. Consequently BCDD caused apoptotic death in cervical cancer cells as evidenced by DNA fragmentation and PARP-cleavage. Further, BCDD did not affect the extrinsic signalling transduction pathway as depicted by its null effect on caspase-8. The in vivo anticancer activity of BCDD was investigated in Ehrlich Ascites carcinoma model where it exhibited tumor regression by 48% at 30 mg/kg, i.p.,in mice. These findings indicated that BCDD is a potential candidate that may be found useful in the management of cervical cancer
EMS-induced cytomictic variability in safflower (Carthamus tinctorius L.)
Seeds of safflower (Carthamus tinctorius L.) were subjected to three treatment durations (3h, 5h and 7h) of 0.5 % Ethyl Methane Sulphonate (EMS). Microsporogenesis was carried out in the control as well as in the treated materials. EMS treated plants showed interesting feature of partial inter-meiocyte chromatin migration through channel formation, beak formation or direct cell fusion. Another interesting feature noticed during the study was the fusion among tetrads due to wall dissolution. The phenomenon of cytomixis was recorded at nearly all the stages of microsporogenesis connecting from a few to several meiocytes. Other abnormalities such as laggards, precocious movement, bridge and non disjunction of chromosomes were also recorded but in very low frequencies. The phenomenon of cytomixis increased along with the increase in treatment duration of EMS. Cells with these types of cytomictic disturbances may probably result in uneven formation of gametes or zygote, heterogenous sized pollen grains or even loss of fertility in future.Π‘Π΅ΠΌΠ΅Π½Π° ΡΠ°ΡΠ»ΠΎΡΠ° (Carthamus tinctorius L.) ΠΎΠ±ΡΠ°Π±Π°ΡΡΠ²Π°Π»ΠΈ 0.5%-Π½ΡΠΌ ΡΡΠΈΠ»ΠΌΠ΅ΡΠ°Π½ΡΡΠ»ΡΡΠΎΠ½Π°ΡΠΎΠΌ (ΠΠΠ‘) Π² ΠΎΠ΄Π½ΠΎΠΌ ΠΈΠ· ΡΡΠ΅Ρ
ΡΠ΅ΠΆΠΈΠΌΠΎΠ² β 3, 5 ΠΈ 7 Ρ. ΠΠΈΠΊΡΠΎΡΠΏΠΎΡΠΎΠ³Π΅Π½Π΅Π· ΠΈΠ·ΡΡΠ°Π»ΠΈ ΠΊΠ°ΠΊ Π² ΠΊΠΎΠ½ΡΡΠΎΠ»Π΅, ΡΠ°ΠΊ ΠΈ Π² ΠΎΠ±ΡΠ°Π±ΠΎΡΠ°Π½Π½ΠΎΠΌ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»Π΅. Π Π°ΡΡΠ΅Π½ΠΈΡ, ΠΎΠ±ΡΠ°Π±ΠΎΡΠ°Π½Π½ΡΠ΅ ΠΠΠ‘, ΠΏΡΠΎΡΠ²Π»ΡΠ»ΠΈ ΠΈΠ½ΡΠ΅ΡΠ΅ΡΠ½ΡΡ ΠΎΡΠΎΠ±Π΅Π½Π½ΠΎΡΡΡ ΡΠ°ΡΡΠΈΡΠ½ΠΎΠΉ ΠΈΠ½ΡΠ΅ΡΠΌΠ΅ΠΉΠΎΡΠΈΡΠ½ΠΎΠΉ ΠΌΠΈΠ³ΡΠ°ΡΠΈΠΈ Ρ
ΡΠΎΠΌΠ°ΡΠΈΠ½Π° ΠΏΡΠΈ ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΠΈ ΠΊΠ°Π½Π°Π»ΠΎΠ², ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΠΈ ΠΊΠ»ΡΠ²ΠΎΠΎΠ±ΡΠ°Π·Π½ΠΎΠ³ΠΎ Π²ΡΡΡΡΠΏΠ° ΠΈΠ»ΠΈ ΠΏΡΡΠΌΠΎΠΌ ΡΠ»ΠΈΡΠ½ΠΈΠΈ ΠΊΠ»Π΅ΡΠΎΠΊ. ΠΡΡΠ³ΠΈΠΌ ΠΎΠ±Π½Π°ΡΡΠΆΠ΅Π½Π½ΡΠΌ Π² ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΈ ΡΠ²Π»Π΅Π½ΠΈΠ΅ΠΌ Π±ΡΠ»ΠΎ ΡΠ»ΠΈΡΠ½ΠΈΠ΅ ΡΠ΅ΡΡΠ°Π΄ ΠΈΠ·-Π·Π° ΡΠ°ΡΡΠ²ΠΎΡΠ΅Π½ΠΈΡ ΡΡΠ΅Π½ΠΊΠΈ. Π€Π΅Π½ΠΎΠΌΠ΅Π½ ΡΠΈΡΠΎΠΌΠΈΠΊΡΠΈΡΠ° ΠΎΡΠΌΠ΅ΡΠ°Π»ΡΡ ΠΏΠΎΡΡΠΈ Π½Π° Π²ΡΠ΅Ρ
ΡΡΠ°Π΄ΠΈΡΡ
ΠΌΠΈΠΊΡΠΎΡΠΏΠΎΡΠΎΠ³Π΅Π½Π΅Π·Π° ΠΈ Π·Π°ΡΡΠ°Π³ΠΈΠ²Π°Π» ΠΎΡ Π½Π΅ΡΠΊΠΎΠ»ΡΠΊΠΈΡ
Π΄ΠΎ ΠΌΠ½ΠΎΠ³ΠΈΡ
ΠΌΠ΅ΠΉΠΎΡΠΈΡΠΎΠ². ΠΡΡΠ³ΠΈΠ΅ Π°Π½ΠΎΠΌΠ°Π»ΠΈΠΈ, ΡΠ°ΠΊΠΈΠ΅ ΠΊΠ°ΠΊ ΠΎΡΡΡΠ°Π²Π°Π½ΠΈΡ, ΠΏΡΠ΅ΠΆΠ΄Π΅Π²ΡΠ΅ΠΌΠ΅Π½Π½ΡΠ΅ Π΄Π²ΠΈΠΆΠ΅Π½ΠΈΡ, ΠΌΠΎΡΡΡ ΠΈ Π½Π΅ΡΠ°Π·Π΄Π΅Π»Π΅Π½ΠΈΡ Ρ
ΡΠΎΠΌΠΎΡΠΎΠΌ, ΠΎΡΠΌΠ΅ΡΠ°Π»ΠΈΡΡ Ρ Π½Π΅Π·Π½Π°ΡΠΈΡΠ΅Π»ΡΠ½ΠΎΠΉ ΡΠ°ΡΡΠΎΡΠΎΠΉ. Π€Π΅Π½ΠΎΠΌΠ΅Π½ ΡΠΈΡΠΎΠΌΠΈΠΊΡΠΈΡΠ° Π²ΠΎΠ·ΡΠ°ΡΡΠ°Π» Ρ ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΠ΅ΠΌ Π΄Π»ΠΈΡΠ΅Π»ΡΠ½ΠΎΡΡΠΈ ΠΎΠ±ΡΠ°Π±ΠΎΡΠΊΠΈ ΠΠΠ‘. ΠΠ»Π΅ΡΠΊΠΈ Ρ ΡΡΠΈΠΌΠΈ ΡΠΈΠΏΠ°ΠΌΠΈ ΡΠΈΡΠΎΠΌΠΈΠΊΡΠΈΡΠ΅ΡΠΊΠΈΡ
Π½Π°ΡΡΡΠ΅Π½ΠΈΠΉ ΠΌΠΎΠ³ΡΡ, Π²Π΅ΡΠΎΡΡΠ½ΠΎ, ΠΏΡΠΈΠ²ΠΎΠ΄ΠΈΡΡ ΠΊ Π½Π΅ΡΠ΅Π³ΡΠ»ΡΡΠ½ΠΎΠΌΡ ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΡ Π³Π°ΠΌΠ΅Ρ ΠΈΠ»ΠΈ Π·ΠΈΠ³ΠΎΡ, Π³Π΅ΡΠ΅ΡΠΎΠ³Π΅Π½Π½ΡΡ
ΠΏΠΎ ΡΠ°Π·ΠΌΠ΅ΡΡ ΠΏΡΠ»ΡΡΠ΅Π²ΡΡ
Π·Π΅ΡΠ΅Π½, ΠΈΠ»ΠΈ Π΄Π°ΠΆΠ΅ ΠΊ ΠΏΠΎΡΠ΅ΡΠ΅ ΡΠ΅ΡΡΠΈΠ»ΡΠ½ΠΎΡΡΠΈ Π² Π±ΡΠ΄ΡΡΠ΅ΠΌ.ΠΠ°ΡΡΠ½Π½Ρ ΡΠ°ΡΠ»ΠΎΡΡ (Carthamus tinctorius L.) ΠΎΠ±ΡΠΎΠ±Π»ΡΠ»ΠΈ 0.5%-Π½ΠΈΠΌ Π΅ΡΠΈΠ»ΠΌΠ΅ΡΠ°Π½ΡΡΠ»ΡΡΠΎΠ½Π°ΡΠΎΠΌ (ΠΠΠ‘) Π² ΠΎΠ΄Π½ΠΎΠΌΡ Π· ΡΡΡΠΎΡ
ΡΠ΅ΠΆΠΈΠΌΡΠ² β 3, 5 Ρ 7 Π³ΠΎΠ΄. ΠΡΠΊΡΠΎΡΠΏΠΎΡΠΎΠ³Π΅Π½Π΅Π· Π²ΠΈΠ²ΡΠ°Π»ΠΈ ΡΠΊ Ρ ΠΊΠΎΠ½ΡΡΠΎΠ»Ρ, ΡΠ°ΠΊ Ρ Π² ΠΎΠ±ΡΠΎΠ±Π»Π΅Π½ΠΎΠΌΡ ΠΌΠ°ΡΠ΅ΡΡΠ°Π»Ρ. Π ΠΎΡΠ»ΠΈΠ½ΠΈ, ΠΎΠ±ΡΠΎΠ±Π»Π΅Π½Ρ ΠΠΠ‘, Π²ΠΈΡΠ²Π»ΡΠ»ΠΈ ΡΡΠΊΠ°Π²Ρ ΠΎΡΠΎΠ±Π»ΠΈΠ²ΡΡΡΡ ΡΠ°ΡΡΠΊΠΎΠ²ΠΎΡ ΡΠ½ΡΠ΅ΡΠΌΠ΅ΠΉΠΎΡΠΈΡΠ½ΠΎΡ ΠΌΡΠ³ΡΠ°ΡΡΡ Ρ
ΡΠΎΠΌΠ°ΡΠΈΠ½Ρ ΠΏΡΠΈ ΡΠΎΡΠΌΡΠ²Π°Π½Π½Ρ ΠΊΠ°Π½Π°Π»ΡΠ², ΡΡΠ²ΠΎΡΠ΅Π½Π½Ρ Π΄Π·ΡΠ±ΠΎΠΏΠΎΠ΄ΡΠ±Π½ΠΎΠ³ΠΎ ΡΠΈ ΠΏΡΡΠΌΠΎΠΌΡ Π·Π»ΠΈΡΡΡ ΠΊΠ»ΡΡΠΈΠ½. ΠΠ½ΡΠΈΠΌ Π²ΠΈΡΠ²Π»Π΅Π½ΠΈΠΌ Π² Π΄ΠΎΡΠ»ΡΠ΄ΠΆΠ΅Π½Π½Ρ ΡΠ²ΠΈΡΠ΅ΠΌ Π±ΡΠ»ΠΎ Π·Π»ΠΈΡΡΡ ΡΠ΅ΡΡΠ°Π΄ ΡΠ΅ΡΠ΅Π· ΡΠΎΠ·ΡΠΈΠ½Π΅Π½Π½Ρ ΡΡΡΠ½ΠΊΠΈ. Π€Π΅Π½ΠΎΠΌΠ΅Π½ ΡΠΈΡΠΎ- ΠΌΡΠΊΡΠΈΡΡ Π²ΡΠ΄Π·Π½Π°ΡΠ°Π²ΡΡ ΠΌΠ°ΠΉΠΆΠ΅ Π½Π° Π²ΡΡΡ
ΡΡΠ°Π΄ΡΡΡ
ΠΌΡΠΊΡΠΎ-ΡΠΏΠΎΡΠΎΠ³Π΅Π½Π΅Π·Ρ Ρ Π·Π°ΡΡΠΏΠ°Π² Π²ΡΠ΄ ΠΊΡΠ»ΡΠΊΠΎΡ
Π΄ΠΎ Π±Π°Π³Π°ΡΡΠΎΡ
ΠΌΠ΅ΠΉΠΎΡΠΈΡΡΠ². ΠΠ½ΡΡ Π°Π½ΠΎΠΌΠ°Π»ΡΡ, ΡΠ°ΠΊΡ ΡΠΊ Π²ΡΠ΄ΡΡΠ°Π²Π°Π½Π½Ρ, ΠΏΠ΅ΡΠ΅Π΄ΡΠ°ΡΠ½Ρ ΡΡΡ
ΠΈ, ΠΌΠΎΡΡΠΈ Ρ Π½Π΅ΡΠΎΠ·Π΄ΡΠ»Π΅Π½Π½Ρ Ρ
ΡΠΎΠΌΠΎΡΠΎΠΌ, Π·ΡΡΡΡΡΡΠ°Π»ΠΈΡΡ Π· Π½Π΅Π·Π½Π°Ρ- Π½ΠΎΡ ΡΠ°ΡΡΠΎΡΠΎΡ. Π€Π΅Π½ΠΎΠΌΠ΅Π½ ΡΠΈΡΠΎΠΌΡΠΊΡΠΈΡΡ Π·ΡΠΎΡΡΠ°Π² ΡΠ· Π·Π±ΡΠ»ΡΡΠ΅Π½Π½ΡΠΌ ΡΡΠΈΠ²Π°Π»ΠΎΡΡΡ ΠΎΠ±ΡΠΎΠ±ΠΊΠΈ ΠΠΠ‘. ΠΠ»ΡΡΠΈΠ½ΠΈ Π· ΡΠΈΠΌΠΈ ΡΠΈΠΏΠ°ΠΌΠΈ ΡΠΈΡΠΎΠΌΡΠΊΡΠΈΡΠ½ΠΈΡ
ΠΏΠΎΡΡΡΠ΅Π½Ρ ΠΌΠΎΠΆΡΡΡ, ΠΉΠΌΠΎΠ²ΡΡΠ½ΠΎ, ΠΏΡΠΈΠ²ΠΎΠ΄ΠΈΡΠΈ Π΄ΠΎ Π½Π΅ΡΠ΅Π³ΡΠ»ΡΡΠ½ΠΎΠ³ΠΎ ΡΠΎΡΠΌΡΠ²Π°Π½Π½Ρ Π³Π°ΠΌΠ΅Ρ Π°Π±ΠΎ Π·ΠΈΠ³ΠΎΡ, Π³Π΅ΡΠ΅ΡΠΎΠ³Π΅Π½Π½ΠΈΡ
Π·Π° ΡΠΎΠ·ΠΌΡΡΠΎΠΌ ΠΏΠΈΠ»ΠΊΠΎΠ²ΠΈΡ
Π·Π΅ΡΠ΅Π½, Π°Π±ΠΎ Π½Π°Π²ΡΡΡ Π΄ΠΎ Π²ΡΡΠ°ΡΠΈ ΡΠ΅ΡΡΠΈΠ»ΡΠ½ΠΎΡΡΡ Π² ΠΌΠ°ΠΉΠ±ΡΡΠ½ΡΠΎΠΌΡ