Influence of the Physicomechanical Properties of Starches on Their Tabletability—A Multivariate Analysis

Purpose The goal of this study was to identify correlations between the physicomechanical properties of different grades of starches with their tabletability. Methods Corn-starch grades (PURE-DENT® B700, PURE-DENT® B810, and PURE-DENT® B830) and pregelatinized corn-starch grades (SPRESS® B818, SPRESS® B820, and SPRESS® B825) were studied for physicomechanical properties, dynamic sorption isotherm, moisture content [MC] (% w/w), dehydration enthalpy (J/g) [ΔHd], and percent crystallinity (%). Tablets (6 mm) were compressed from hand-weighed powders (constant true volume) using Gamlen Tablet Press (Compression pressure-100 MPa; Compression speed- 5mm/s, 50 mm/s). Tablet mechanical strength (TMS) and Heckel parameters were evaluated. Correlation between physicomechanical properties and compression descriptors was evaluated by multivariate method. Results All starches followed Type-III sorption isotherm with open hysteresis loop indicating their large amorphous content. High amorphous content was further confirmed with hollow diffraction peaks of starches in the powder X-ray diffraction studies. Glass transition temperature of all starches was about 101°C. The moisture content and percent crystallinity of all starches was found statistically insignificant. However, PURE-DENT® B830 and SPRESS® B818 showed significantly low ΔHd values. Principle component analysis (PCA) loadings plot calculated with measured physicomechanical properties and TMS showed positive correlation between high Heckel Yield pressure values of plastic and elastic deformation and negative correlation with percent crystallinity, ΔHd, and MC along PC1. These relationships confirmed expected phenomenon in PCA score plots that Starches (PURE-DENT® B830 and SPRESS® B818) with plastic deformation followed by low elastic recovery in the decompression phase shows better tabletability. Furthermore, positive correlation of low ΔHd with TMS might indicate that starches with easy availability of associated water (low ΔHd) might have better tabletability due to water induced material plasticization. Conclusion Out of the six different grades of starches studied PURE-DENT® B830 and SPRESS® B818 showed better tabletability regardless of similar MC and amorphous nature. The better tabletability of these two starches might be attributed to their better plasticization due to loosely bound associated water, and low elastic recovery in the decompression phase

Neutrinos, Axions and Conformal Symmetry

We demonstrate that radiative breaking of conformal symmetry (and simultaneously electroweak symmetry) in the Standard Model with right-chiral neutrinos and a minimally enlarged scalar sector induces spontaneous breaking of lepton number symmetry, which naturally gives rise to an axion-like particle with some unusual features. The couplings of this `axion' to Standard Model particles, in particular photons and gluons, are entirely determined (and computable) via the conformal anomaly, and their smallness turns out to be directly related to the smallness of the masses of light neutrinos.Comment: 10 pages, 2 figures, expanded version, to be published in EPJ

Inhibition of NUCKS Facilitates Corneal Recovery Following Alkali Burn

Corneal wound healing involves a complex cascade of cytokine-controlled cellular events, including inflammatory and angiogenesis responses that are regulated by transcriptional chromatin remodeling. Nuclear Ubiquitous Casein and cyclin-dependent Kinase Substrate (NUCKS) is a key chromatin modifier and transcriptional regulator of metabolic signaling. In this study, we investigated the role of NUCKS in corneal wound healing by comparing its effects on corneal alkali burn in NUCKS knockout (NKO) and NUCKS wild-type (NWT) mice. Our data showed that following alkali-injury, inhibition of NUCKS (NKO) accelerated ocular resurfacing and suppressed neovascularization; the cytokine profile of alkali burned corneas in NKO mice showed suppressed expression of inflammation cytokines (IL1A &IL1B); upregulated expression of antiangiogenic factor (Pigment Epithelium-derived Factor; PEDF); and downregulated expression of angiogenic factor (Vascular Endothelial Growth Factor, VEGF); in vitro, following LPS-induced NFκB activation, NKO corneal cells showed reduced expression of IL6, IP10 and TNFα. In vitro, corneal epithelial cells showed reduced NF-κb activation on silencing of NUCKS and corresponding NFκB-mediated cytokine expression was reduced. Here, we illustrate that inhibition of NUCKS played a role in cytokine modulation and facilitated corneal recovery. This reveals a potential new effective strategy for ocular burn treatment.published_or_final_versio

Post-mortem examination of Hospital Inpatient COVID-19 Deaths in Lusaka, Zambia - A Descriptive Whole-body Autopsy Series

BACKGROUND: Since information on the pathology of COVID-19 from sub-Saharan Africa (SSA) remains scarce, the objective of our study was to define the gross pathology and histological features of COVID-19. We report data from 29 whole-body autopsies of COVID-19 deaths occurring in hospitals in Lusaka, Zambia - the first large autopsy case series from Africa. METHODS: We performed a descriptive post-mortem examination study of inpatient COVID-19 related deaths at two hospitals in Lusaka, Zambia. Whole-body autopsies were conducted according to Standard Operating Procedures. Gross and histopathological examinations of all organs were performed. Patient demographics, history, co-morbidities, autopsy gross and microscopic findings, and cause(s) of death were recorded and analyzed using STATA version 14. Variables were grouped and presented as frequencies and percentages. FINDINGS: Autopsies were performed on 29 decedents (mean age = 44 ± 15.8years; age range = 19-82; 17/29 [58.8%] males). 22/29 [75.9%] cases were <55 years of age. A spectrum of pathological manifestations of COVID-19 were seen in all organs. The commonest causes of death were pulmonary thromboembolism (13/29, 45%), Diffuse Alveolar Damage (9/29, 31%), and COVID-19 pneumonia (7/29, 25%). 22/29 (76%) had co-morbidities. Common co-morbidities included HIV (8/29, 28%), Hypertension (6/29, 20%) Tuberculosis (3/29, 10%), Diabetes (3/29, 10%). CONCLUSIONS: A spectrum of gross anatomical and histopathological findings are seen in COVID-19 deaths in hospitalized decedents. These appear broadly similar to those reported from China, Europe and USA. Differences include a younger age group, and co-morbidities of HIV and TB co-infection which require further investigation

Spatial and temporal analysis of haemorrhagic septicaemia outbreaks in India over three decades (1987–2016)

Haemorrhagic septicaemia (HS) is an economically important disease affecting cattle and buffaloes and the livelihoods of small-holder farmers that depend upon them. The disease is caused by Gram-negative bacterium, Pasteurella multocida, and is considered to be endemic in many states of India with more than 25,000 outbreaks in the past three decades. Currently, there is no national policy for control of HS in India. In this study, we analysed thirty year (1987–2016) monthly data on HS outbreaks using different statistical and mathematical methods to identify spatial variability and temporal patterns (seasonality, periodicity). There was zonal variation in the trend and seasonality of HS outbreaks. Overall, South zone reported maximum proportion of the outbreaks (70.2%), followed by East zone (7.2%), Central zone (6.4%), North zone (5.6%), West zone (5.5%) and North-East zone (4.9%). Annual state level analysis indicated that the reporting of HS outbreaks started at different years independently and there was no apparent transmission between the states. The results of the current study are useful for the policy makers to design national control programme on HS in India and implement state specific strategies. Further, our study and strategies could aid in implementation of similar approaches in HS endemic tropical countries around the world

Incidental Tuberculosis in sudden, unexpected, and violent deaths in the community Lusaka, Zambia - A descriptive forensic post-mortem examination study

Objectives: Tuberculosis remains a global emergency. In Zambia only 55% of tuberculosis cases are diagnosed. We performed a study to determine incidental cases of tuberculosis seen at forensic autopsy of individuals who died suddenly and unexpectedly in the community in Lusaka, Zambia. Methods: Whole-body autopsies were performed according to Standard Operating Procedures. Representative samples obtained from relevant organs were subjected to pathological examination. Information on circumstances surrounding the death was obtained. Data on patient demographics, gross and microscopic pathological findings, and cause(s) of death were analysed. Results: Incidental tuberculosis was found in 52 cases (45 male, 7 female, age range 14-66) out of 4286 whole-body autopsies. 41/52 (80%) were aged 21-50 years. One was a 14-year old boy who died during a football match. 39/52 (75%) deaths were attributable specifically to tuberculosis only. Other deaths were due to acute alcohol intoxication(4), violence(7), ruptured ectopic pregnancy(1), bacterial meningitis (1). All the cases were from poor socio-economic backgrounds and lived in high-density areas of Lusaka. Conclusions: Incidental cases of active tuberculosis undiagnosed antemortem seen at forensic autopsy reflects major gaps in the national TB control programs. More investments into proactive screening, testing, treatment activities, and accurate data collection are required