Interaction between Meloidogyne javanica and Pseudomonas solanacearum on brinjal

The combined pathogenic effects of Pseudomonas solanacearum biotype-3 and Meloidogynejavanha on brinjal were greater than independent effects of either. The most severe wilt development occurred in plants inoculated with the nematode, two and three weeks prior to bacterial inoculation than simultaneous inoculation or inoculation of bacteria four weeks afler the nematode inoculation. A minimum of 10 egg-masses! plal1t facilitated wilt development, six days after bacterial inoculation. Increased nematode inoculum levels 50,100 and 150 egg-masses/plant hastelled the wilt symptom development. Rgher nematode inoculum levels (100 and 150 egg-masses/plant) significantly reduced plant growth Bacterial inoculation alone in the root zone did not affect growth parameters

Relative effectiveness of different inoculation techniques with bacterial wilt pathogen, Pseudomonas solanacearum-biotype 3, on brinjal

The wilt incidence by Pseudomonas solanacearum Smith has always been erratic following artificial inoculations, probably due to difficulty in maintaining viruloence of the pathogen as well as the influence of certain factors such as soil moisture and temperature. Root and stem inoculation with the pathogen did not make and difference on typical final wilt symptoms. This paper reports the results of inoculation techniques for uniform and rapid development of bacterial wilt in brinjal (Solanum melongena ) for selecting promising resistant varieties

Relative efficacy of some selected antibiotics on bacterial wilt(Pseudomonas solanacearum biotype-3) of brinjal

Five antibiotics were evaluated in vivo against the bacterial wilt of brinjal (Solanum melongena ) caused by Pseudomonas solanacearum biotype-3. Penicillin and agrimycin-100 were consistently superior over other antibiotics against the pathogen when dispensed into the Hoagland's solution before planting. Foliar application of agrimycin-100, chloramphenicol and streptomycin sulphate a day prior to bacterial inoculation were effective at higher concentrations. Penicillin and tetracycline did not inhibit the wilt bacteria at any of the concentration tested. None of the antibiotics could check the disease when sprayed a day after bacterial inoculation

Histological aspects of Pseudomonas and root-knot nematode wilt complex in brinjal

Histological studies of brinjal roots inoculated with Meloidogyne javanica, two or three weeks before Pseudomonas solanacearum biotype-3 inoculation showed extensive cavities of broken cortical and endodermal cells. The nematode inoculated roots contained pronounced hyperplastic and hypertrophic regions characterised by 'giant cells' with dense cytoplasm and enlarged nuclei. The cellular changes (hypertrophy and hyperplasia) resulted in syncytial formation. Development in the nematode inoculated roots predisposed the bacterial invasion and colonization

Simple Method for Enrichment of Azadirachtin from Neem Seeds

990-994Azadirachtin the principle active constituent of Azadirachta indica ranges from 0.3 to 0.6 per cent. A simplified procedure, when compared to established methods, has been adopted to enrich azadirachtin ‘A’ to 50 per cent using minimum possible steps without resorting to chromatographic techniques. It involves additional partition with CCl4 before final extraction which removes most of nimbin, salanin, and allied triterpenoids. Enriched product finds immense utility in preparing formulations with high azadirachtin and also in isolating pure azadirachtin by adopting preparative HPLC