Colposcopic evaluation and papanicolaou smear in high risk groups and its correlation with histology

Background: Cervical cancer is one of the most common gynaecologic neoplasms. PAP smear and colposcopy are used for its early detection. This study aims to find the correlation of colposcopic evaluation with Pap smear in cervical cancer screening and with histology. Methods: All women attending the OPD with unhealthy cervix and abnormal symptoms, who gave written informed consent were included in the study. Pap smear cytological grading, colposcopic findings were recorded. Pap smear and colposcopy findings was compared with histopathology. Results: The study included 73 patients. Pap smear was negative in more than half of the patients (56.2%), followed by atypical squamous cells of undetermined significance in 12 (16.4%), low-grade squamous intraepithelial lesion in 17 (23.3%), high-grade squamous intraepithelial lesion in 2 (2.7%) and squamous cell carcinoma in 1 patient (1.4%).  The histopathology showed normal findings in 46 patients (63%), followed by CIN 1 in 11 (15.1%), CIN 2 in 6 patients (8.2%), CIN 3 and squamous cell carcinoma in 5 patients each (6.8%). Pap smear’s predictability of cervical malignancy showed that it had a sensitivity of 48.15, it’s specificity for identifying patients without cervical malignancy was 84.78%. Colposcopy’s predictability of cervical malignancy showed that it had a sensitivity of 88.89%, it’s specificity for identifying patients without cervical malignancy was 95.65%. Conclusions: Colposcopy does seem to be better than Pap smear in diagnosing cervical carcinoma and also identifying patients without it

The formation of supermassive black holes in the first galaxies

We discuss the formation of supermassive black holes in the early universe, and how to probe their subsequent evolution with the upcoming mm/sub-mm telescope ALMA. We first focus on the chemical and radiative conditions for black hole formation, in particular considering radiation trapping and molecular dissociation effects. We then turn our attention towards the magnetic properties in the halos where the first black holes form, and show that the presence of turbulence may lead to a magnetic dynamo, which could support the black hole formation process by providing an efficient means of transporting the angular momentum. We finally focus on observable properties of high-redshift black holes with respect to ALMA, and discuss how to distinguish between chemistry driven by the starburst and chemistry driven by X-rays from the black hole.Comment: Contribution to AIP conference proceedings "First Stars and Galaxies: Challenges in the Next Decade". 4 pages, 3 figure

Knowledge and Awareness of usage of Artificial Sweeteners among Indian type 2 diabetes individuals in a tertiary diabetes institute

Background: Diabetes mellitus is a chronic metabolic disease primarily attributable to unhealthy, untimely food habits and lack of physical activity. Good glycemic control is one of the key aspects in preventing complications. This has led to a shift in replacing sugar with artificial sweeteners amongst the diabetic population. It is not known whether this is aimed to maintain blood glucose levels or to satisfy the sweet cravings. There is lack of awareness of the type and long-term side effects of artificial sweeteners among people with diabetes. Objectives: This study was conducted to assess the awareness and knowledge of usage of artificial sweeteners among adults with type 2 diabetes visiting a tertiary diabetes institute. Materials and Methods: The study population involved 297 adults (≥18 years) with type 2 diabetes attending tertiary diabetes institute. Data was collected from face-to-face interview technique along with a pre-validated questionnaire. Results: The total number of subjects (n=297) comprised 126 females and 171 males of age 18-88 with mean age of 56.5 years. Sucralose was the most popular sugar substitute amongst the subjects (45%) followed by Aspartame (32%), 13% of them are completely not aware of the type of artificial sweetener that they consumed. About 36.7% of the subjects belonged to pre-obese category with BMI 25-29.9kg/m2 with women on the upper scale. 57.91% of the respondents started consuming artificial sweeteners in the recent years i.e., between 1to 5 years. 87% of the subjects consumed artificial sweeteners in the form of pellets in tea or coffee as a medium and 51% consumed it to manage blood glucose levels. 51.2% had gastrointestinal side effects. Significant number of the subjects (81.5%) was unaware of the long-term side effects of artificial sweeteners. Conclusions: The study findings highlight the high rate of unawareness amongst the subjects regarding the side effects of long-term consumption of artificial sweeteners. Hence, reading nutrition label on the products, judicious consumption of artificial sweeteners and nutritional education can be helpful in making wise food choices

STANDARDIZATION AND APPLICATION OF PCR TARGETING CHLORELLA SPECIES ISOLATED FROM ENVIRONMENTAL SAMPLES

Objective: Identification of Chlorella species from the environment through 18s ribosomal RNA sequencing. This study was aimed to design primer targeting Chlorella and other closely related algal species targeting 18s ribosomal RNA, ITS1 region. Methods: Sanger sequencing was carried out for the identification of algae up to the genus and species level using an in-house designed primer and optimized PCR conditions. Results: Out of 2 algae samples identified phenotypically, one isolate identified as Chlorella vulgaris and other one identified as Chlorella sorokiniana based on the results of Basic Alignment Search Tool (BLAST). Conclusion: To conclude, this study provided primers with PCR conditions to characterize algal samples through molecular identification with 100% accuracy than the phenotypic method

Anesthetic Management of a Patient with Ebstein's Anomaly for Obstructed Inguinal Hernia

Ebstein’s anomaly is a rare congenital heart disease constituting less than 1% of patients with congenital heart diseases.  It has diverse presentations, severity, and consequently, the management too. These patients are at increased risk of developing ventricular and supraventricular tachycardias, congestive heart failure, and even death. Here, we report a case of a 32-year old man with Ebstein’s anomaly, who had successfully undergone emergency open inguinal hernioplasty under nerve blocks

Maternal pregnancy associated plasma protein-A (PAPP-A) and uterine artery Doppler changes as predictors of pre-eclampsia: a prospective observational study from a teaching hospital in Mysore, Karnataka, India

Background: Hypertensive disorder affects 10-12% of pregnancies. Identifying women, who are at risk is conducive to prompt gestational management. PAPP-A is a protein complex produced by the developing trophoblasts. Low levels of PAPP-A at 10–14 weeks is a marker of impaired placentation and a smaller placental mass. Doppler imaging permits non-invasive evaluation of the uteroplacental circulation and is invaluable in the management of high-risk pregnancies. The uterine artery Doppler screening identifies patients at risk for developing preeclampsia. To study the association of PAPP-A and the uterine artery Doppler changes as predictor of pre-eclampsia in pregnant women at 11-14 weeks of gestation.Methods: This was a prospective study of 150 pregnant women presenting at 11-14 weeks of gestation for a prenatal check-up. After considering the inclusion and exclusion criteria, serum samples for PAPP-A were assayed. Ultrasound Doppler was used to obtain uterine artery flow velocity waveforms and mean pulsatility index and resistance index of uterine arteries were calculated. Cases were followed up till term and observed for development of pre-eclampsia.Results: 48.6% had low serum PAPP-A levels, in which 77% developed PE. The Mean PI and RI is 2.34±1.16 and 0.58±0.1 respectively. 30% women with abnormal PI values and 24% of women with abnormal RI values developed PE.Conclusions: The combination of maternal history with low serum PAPP-A levels and abnormal uterine artery Doppler at 11-14 weeks can be used as predictor of pre-eclampsia

MANAGING AND STORAGE DETECTION OF EFFECTIVE RE PROTECT IN WSNS

Using the Copy Recognition procedure, it is designed to increase the recognition. Objective will a procedure for the Recognition of Distributed Copies with Random Selection of Witnesses to increase the probability of identifying cloning as an impact on the useful life of the network and reduce the advantages of buffering data storage. The loop structure facilitates the transmission of energy saving data through the path for both witnesses and for good. Theoretically, we show that the protocol is capable of achieving 100% of the possibility of identifying cloning with reliable controls. In particular, we use the location information of the sensors and the random selection tokens located at the location of the diamond ring to verify the sensors and also to report the detected cloning attacks. In addition, in many replication identification protocols that exist with the Random Witness Selection Scheme, the required caching of the sensors is generally determined by the density of the node. The complete simulation shows that can effectively extend the life of the network by effectively distributing traffic through the network. The current system does not ensure that at least one of the witnesses can see the identity of the sensor points to see if there is a clone attack or not. The performance of the ERCD is evaluated when it is possible to identify cloning, power consumption, age of the network and the capacity of the knowledge store. The broad results of the simulation show that ERCD protocol is capable of offering superior performance in terms of user recognition and network life with a reasonable data storage capacity