Автоматическая оптимизация технологического комплекса обогащения железных руд по сигналам магнитной индукции сепаратора

Recombinant antibodies can be used to diagnose, treat and prevent disease by exploiting their specific antigen-binding activities. A large number of drugs currently in development are recombinant antibodies and most of these are produced in cultured rodent cells. Although such cells produce authentic functional products, they are expensive, difficult to scale-up and may contain human pathogens. Plants represent a cost-effective, convenient and safe alternative production system and are slowly gaining acceptance. Five plant-derived therapeutic recombinant antibodies (plantibodies) are undergoing clinical evaluation, three of which can be used as prophylactics

Molecular farming of human tissue transglutaminase in tobacco plants

In this study we have utilized Nicotiana tabacum with a molecular farming purpose in attempt of producing transgenic plants expressing the human tissue transglutaminase (htTG). Three plant expression constructs were used enabling targeting and accumulation of the recombinant protein into the plant cell cytosol (cyto), the chloroplasts (chl) and the apoplastic space (apo). Analysis of transgenic T(0) plants revealed that recombinant htTG was detectable in all three transgenic lines and the accumulation levels were in a range of 18-75 mu g/g of leaf material. In the T(1) generation, the recombinant htTG was still expressed at high level and a significant catalytic activity was detected into the leaf protein extracts. Southern blot analyses revealed that apo and chl plants of T(1) generation possess a high copy number of the recombinant htTG in their genome, while the cyto plants carry a single copy

Правові засади здійснення діяльності зі спільного інвестування

We describe the expression of the bispecific antibody biscFv2429 in transgenic suspension culture cells and tobacco plants. biscFv2429 consists of two single chain antibodies, scFv24 and scFv29, connected by the Trichoderma reesi cellobiohydrolase I linker. biscFv2429 binds two epitopes of tobacco mosaic virus (TMV): the scFv24 domain recognizes neotopes of intact virions, and the scFv29 domain recognizes a cryptotope of the TMV coat protein monomer. biscFv2429 was functionally expressed either in the cytosol (biscFv2429-cyt) or targeted to the apoplast using a murine leader peptide sequence (biscFv2429-apoplast). A third construct contained the C-terminal KDEL sequence for retention in the ER (biscFv2429-KDEL). Levels of cytoplasmic biscFv2429 expression levels were low. The highest levels of antibody expression were for apoplast targeted biscFv2429-apoplast and ER retained biscFv2429-KDEL, which reached a maximum expression level of 1.65% total soluble protein in transgenic plants. P lant expressed biscFv2429 retained both epitope specificities, and bispecificity and bivalency were confirmed by ELISA and surface plasmon resonance analysis. This study establishes plant cells as an expression system for bispecific single chain antibodies for use in medical and biological applications

Связь микроэлементного состава Spirodela polyrhiza (L.) Schleid со средой его обитания

Plants and plant cell cultures have been evaluated over the past two decades as alternative production platforms for biopharmaceuticals. A large number of candidate proteins has been successfully produced in a range of plant species and the most advanced products are currently progressing through the clinical development stages. We first discuss principles for the production of plant-made pharmaceuticals including plant transformation procedures, post-translational modifications, and downstream processing. We then highlight some of the most advanced plant-made biopharmaceuticals currently in clinical development for human medical use

Создание полимерных композиционных материалов с непрерывным армированием арамидным волокном для применения в аддитивных технологиях

В данной работе проведено исследование создания композиционного материала на полимерной основе с непрерывным армированием арамидного волокна для применения в аддитивных технологиях. Описаны технологические особенности процесса модификации поверхности арамидного волокна. Исследовано влияние материала модификации поверхности на адгезионные свойства получаемого композиционного материала

Разработка математической модели процесса гидроочистки вакуумного дистиллята

Plants are now gaining widespread acceptance as a general platform for the large-scale production of recombinant proteins. The first plant-derived recombinant pharmaceutical proteins are reaching the final stages of clinical evaluation, and many more are in the development pipeline. Over the past two years, there have been some notable technological advances in this flourishing area of applied biotechnology, as shown by the continuing commercial development of novel plant-based expression platforms. There has also been significant success in tackling some of the limitations of plant bioreactors, such as low yields and inconsistent product quality, that have limited the approval of plant-derived pharmaceuticals

Оценка возможности метода КИМов для решения характерных задач по изменению структуры и параметров тепловых схем ПТУ АЭС

Объектом исследования дипломной работы являются экспресс-методы решения характерных задач по изменению структуры тепловых схем ПТУ АЭС. Целью работы является выявление сравнительной оценки результатов расчетов тепловых схем экспресс-методами. Проводились вариантные сравнительные расчеты при изменении структуры и параметров тепловых схем.The object of the research thesis are rapid methods for solving specific problems of restructuring the thermal circuit Vocational plant. The aim is to identify the comparative evaluation of results of calculations of thermal schemes express methods. Were variant comparative calculations when changing the structure and parameters of thermal schemes

Применение буровых шламов в изготовлении строительных материалов

Thrombomodulin is a membrane-bound protein that plays an active role in the blood coagulation system by binding thrombin and initiating the protein C anticoagulant pathway. Solulin (TM) is a recombinant soluble derivative of human thrombomodulin. It is used for the treatment of thrombotic disorders. To evaluate the production of this pharmaceutical protein in plants, expression vectors were generated using four different N-terminal signal peptides. Immunoblot analysis of transiently transformed tobacco leaves showed that intact Solulin (TM) could be detected using three of these signal peptides. Furthermore transgenic tobacco plants and BY2 cells producing Solulin (TM) were generated. Immunoblot experiments showed that Solulin (TM) accumulated to maximum levels of 115 and 27 mu g g(-1)supercript stop plant material in tobacco plants and BY2 cells, respectively. Activity tests performed on the culture supernatant of transformed BY2 cells showed that the secreted Solulin (TM) was functional. In contrast, thrombomodulin activity was not detected in total soluble protein extracts from BY2 cells, probably due to inhibitory effects of substances in the cell extract. N-terminal sequencing was carried out on partially purified Solulin (TM) from the BY2 culture supernatant. The sequence was identical to that of Solulin (TM) produced in Chinese hamster ovary cells, confirming correct processing of the N-terminal signal peptide. We have demonstrated that plants and plant cell cultures can be used as alternative systems for the production of an active recombinant thrombomodulin derivative

Пенное пожаротушение: особенности в современных условиях

В статье показаны преимущества систем пенного пожаротушения. Рассмотрены современные тенденции в пенном пожаротушении. Проанализированы недостатки пенного пожаротушения, обусловленные системой дозирования пенообразователя. Рассмотрены виды дозаторов и предложена электронная система дозирования "Спрут-СД"