HERPETO·FAU'NAL SURVEVOFTHE DEENSTON·NAVANAGALA NATURE TRAIL, THE KNUCKLES RANGE

A survey of amphibians and reptiles was carried out along the "Deenston-NavanagalaNature Trail" on the eastern slopes of the Knuckles Range (elevation: 915-1906 m)during the June-Oct period of 2003. The objective was to identify the amphibians andreptiles species in their micro-habitats of the studies area. The survey is important as SriLanka is an amphibian biodiversity hot spotThe survey was done both during day and night using the 'long transect method'. 'Visiblerange' (weather-dependent) determined the transect width. The species were caught foridentification (using hand nets) and released.12 amphibian species were recorded within three families. There were six endemic - allnationally threatened-including one in the endemic genus Lankanectus . Theirdistribution in the micro-habitat is:Litter: 2 spp.• Puddles: 2 spp.• Stream banks: 3 spp.• Tree holes: I sp.• Trunks: 2 spp,Litter and bushes: I sp., Litter and underneath logs: 1 sp.As for the reptiles the number of tetrapod species is 12 within three families. Out of 8endemic spceis 6 are nationally threatened.There were 17 serpentoid species within 4 families. Six are endemic and nationallythreatened. Their micro-habitat break down is:Tetrapod reptiles:Litter: 2 spp.. Trunks: 1 spp.Cardamom & trunks I sp.• Litter & beneath rocks 1 sp.. Trunk & beneath rocks I sp.•Trunk and Bushes 2 sp., Trunk and Rocks 2 sp.Serpentoid reptiles:Grasslands: 4 spp.• Litter: 5 spp., Log cavities; I sp., Puddles: 1 sp.,Sub persorial : 1 sp.• Tree holes: 1 sp.Grass and litter: I sp. Trunks.and bushes: 3 spp

A STUDY ON THE BUTTERFLY FAUNAL DIVERSITY IN VARIOUS FOREST HABITATS IN THE KNUCKLES FOREST RANGE

A butterfly faunal survey was conducted in the Deenston-Nawanagala Nature Trail areaon the eastern flank of the Knuckles Forest Range at a 915-1906m of elevation with theobjective of studying the butterfly faunal of the area in different habitat types.A field survey was carried out one day per month during August 2002 to August 2003period. Data collection was done using the 'long-transect method' containing thetransect width to the 'visible range' that varied according to the atmospheric conditionsThe following five major habitat types (Listed in the descending order of extent) wereidentified: Abandoned Tea Lands with Lime Grass, Lowland Semi-evergreen Forest, SubMontane Forest, Montane Forest and Abandoned Cardamom AreasThe total number of species recorded was 40 in seven familiesThis habitat distribution is as follows: Abandoned Tea Lands with Lime Grass: 27 spp.,Lowland Semi-evergreen Forest; 28, Sub Montane Forest; 18, Montane Forest; 9,Abandoned Cardamom Areas;13.Their distribution in the families is; Nymphalidae 12 spp., Papilionidae 8, Pieridae 6,Satyridae 6, Danaidae 5, Lycaenidae 2 and Riodinidae 1. Only the Family Nymphalidaeis found in all habitat types.Three of these species are endemic and altogether five species (Including the threeendemics) are nationally threatened.In the study area the butterfly fauna facing threats due to human and natural causes. Manmade fires occur in grasslands where 27 species (54%) are found. Cardamom cultivationis another factor. Strong winds in some seasons and forest dieback (especially inNawangala) are natural causes. The need for conserving this butterfly fauna ishighlighted in this paper

In vivo confocal microscopy features and clinicohistological correlation of limbal nerve corpuscles

Aims To describe the in vivo confocal microscopy (IVCM) features of human limbal nerve corpuscles (LNCs) and correlate these with the histological features.Methods We examined 40 eyes of 29 healthy living subjects (17 female, 12 male; mean age=47.6) by IVCM. Four limbal quadrants were scanned through all epithelial layers and stroma to identify the LNCs and associated nerves. Ten fresh normal human corneoscleral discs from five deceased patients with a mean age of 67 years and 17 eye-bank corneoscleral rims with a mean age of 57.6 years were stained as whole mounts by the acetylcholinesterase (AChE) method to demonstrate LNCs and corneal nerves. Stained tissue was scanned in multiple layers with the NanoZoomer digital pathology microscope. The in vivo results were correlated to the histological findings.Results On IVCM, LNCs were identified in 65% of the eyes studied and were mainly (84%) located in the inferior or superior limbal regions. They appeared either as bright (hyper-reflective) round or oval single structures within the hyporeflective, relatively acellular fibrous core of the palisades or were clustered in groups, often located anterior to the palisades of Vogt. They measured 36 µm in largest diameter (range 20–56 µm). The in vivo features were consistent with the histology, which showed LNCs as strongly AChE positive round or oval structures.Conclusion The strong correlation with histology will enable use of IVCM to study LNCs in normal and disease conditions

Case Report: melioidosis - poor compliance resulting in a relapse

No abstract availabl

Sickle Cell Trait and Kidney Disease in People of African Ancestry With HIV

Introduction: Sickle cell trait (SCT) has been associated with chronic kidney disease (CKD) in African Americans, although evidence for its impact in Africans and people with HIV is currently lacking. We conducted a cross-sectional study investigating the association between SCT and kidney disease in people of African ancestry with HIV in the UK. Methods: The primary outcome was estimated glomerular filtration rate (eGFR) 50 mg/mmol), and albuminuria (albumin-to-creatinine ratio >3 mg/mmol). Multivariable logistic regression was used to estimate the associations between SCT and kidney disease outcomes. Results: A total of 2895 participants (mean age 48.1 [SD 10.3], 57.2% female) were included, of whom 335 (11.6%) had SCT and 352 (12.2%) had eGFR <60 ml/min per 1.73 m2. After adjusting for demographic, HIV, and kidney risk factors including APOL1 high-risk genotype status, individuals with SCT were more likely to have eGFR <60 ml/min per 1.73 m2 (odds ratio 1.62 [95% CI 1.14–2.32]), eGFR <90 ml/min per 1.73 m2 (1.50 [1.14–1.97]), and albuminuria (1.50 [1.09–2.05]). Stratified by APOL1 status, significant associations between SCT and GFR <60 ml/min per 1.73 m2, eGFR <90 ml/min per 1.73 m2, proteinuria, and albuminuria were observed for those with APOL1 low-risk genotypes. Conclusion: Our results extend previously reported associations between SCT and kidney disease to people with HIV. In people of African ancestry with HIV, these associations were largely restricted to those with APOL1 low-risk genotypes

Genetic Variants of APOL1 Are Major Determinants of Kidney Failure in People of African Ancestry With HIV

INTRODUCTION: Variants of the APOL1 gene are associated with chronic kidney disease (CKD) in people of African ancestry, although evidence for their impact in people with HIV are sparse. METHODS: We conducted a cross-sectional study investigating the association between APOL1 renal risk alleles and kidney disease in people of African ancestry with HIV in the UK. The primary outcome was end-stage kidney disease (ESKD; estimated glomerular filtration rate [eGFR] of 30 mg/mmol), and biopsy-proven HIV-associated nephropathy (HIVAN). Multivariable logistic regression was used to estimate the associations between APOL1 high-risk genotypes (G1/G1, G1/G2, G2/G2) and kidney disease outcomes. RESULTS: A total of 2864 participants (mean age 48.1 [SD 10.3], 57.3% female) were genotyped, of whom, 354 (12.4%) had APOL1 high-risk genotypes, and 99 (3.5%) had ESKD. After adjusting for demographic, HIV, and renal risk factors, individuals with APOL1 high-risk genotypes were at increased odds of ESKD (odds ratio [OR] 10.58, 95% CI 6.22–17.99), renal impairment (OR 5.50, 95% CI 3.81–7.95), albuminuria (OR 3.34, 95% CI 2.00–5.56), and HIVAN (OR 30.16, 95% CI 12.48–72.88). An estimated 49% of ESKD was attributable to APOL1 high-risk genotypes. CONCLUSION: APOL1 high-risk genotypes were strongly associated with kidney disease in people of African ancestry with HIV and accounted for approximately half of ESKD cases in this cohort

Investigating Tissue Optical Properties and Texture Descriptors of the Retina in Patients with Multiple Sclerosis

PURPOSE: To assess the differences in texture descriptors and optical properties of retinal tissue layers in patients with multiple sclerosis (MS) and to evaluate their usefulness in the detection of neurodegenerative changes using optical coherence tomography (OCT) image segmentation. PATIENTS AND METHODS: 38 patients with MS were examined using Stratus OCT. The raw macular OCT data were exported and processed using OCTRIMA software. The enrolled eyes were divided into two groups, based on the presence of optic neuritis (ON) in the history (MSON+ group, n = 36 and MSON- group, n = 31). Data of 29 eyes of 24 healthy subjects (H) were used as controls. A total of seven intraretinal layers were segmented and thickness as well as optical parameters such as contrast, fractal dimension, layer index and total reflectance were measured. Mixed-model ANOVA analysis was used for statistical comparisons. RESULTS: Significant thinning of the retinal nerve fiber layer (RNFL), ganglion cell/inner plexiform layer complex (GCL+IPL) and ganglion cell complex (GCC, RNFL+GCL+IPL) was observed between study groups in all comparisons. Significant difference was found in contrast in the RNFL, GCL+IPL, GCC, inner nuclear layer (INL) and outer plexiform layer when comparing MSON+ to the other groups. Higher fractal dimension values were observed in GCL+IPL and INL layers when comparing H vs. MSON+ groups. A significant difference was found in layer index in the RNFL, GCL+IPL and GCC layers in all comparisons. A significant difference was observed in total reflectance in the RNFL, GCL+IPL and GCC layers between the three examination groups. CONCLUSION: Texture and optical properties of the retinal tissue undergo pronounced changes in MS even without optic neuritis. Our results may help to further improve the diagnostic efficacy of OCT in MS and neurodegeneration