PRICKLE1-related early onset epileptic encephalopathy

The PRICKLE1 (Prickle Planar Cell Polarity Protein 1-MIM 608500) gene is involved in different phases of human development. The related diseases include autosomal recessive progressive myoclonus epilepsy - ataxia syndrome, neural tube defects associated with heterozygous mutations, agenesis of corpus callosum, polymicrogyria, and autistic spectrum disorder. Reported here is a young boy with a new variant (NM_153026.2:c.820G>A, p.Ala274Thr) presenting with an early infantile epileptic encephalopathy with developmental arrest

Microbiological retention on PTFE versus silk suture: a quantitative pilot study in third molar surgery

Background: Mandibular third molar (M3M) removal and management of postoperative complications represent a common matter of interest in oral and maxillofacial surgery. This potential quantitative study assessed the ability of two types of surgical sutures, Silk and polytetrafluoroethylene polymer (PTFE), to carry aerobic and anaerobic bacteria on wounds after mandibular third molar surgery, with a collection of the stitches at the suture removal and study in the laboratory on the basis of colony-forming units. Methods: This prospective quantitative study sampled a total of 10 consecutive healthy patients for mandibular third molar surgery at the Oral Surgery School, Dentistry and Dental Prosthodontics, Department of Medical Biotechnologies, University of Siena, Siena, Italy. The mean age of the patients was 31 years (range 25–40 years), seven patients were male and three patients were female. Inclusion criteria were: presence of a partially impacted mandibular third molar. Exclusion criteria were: smoking and diabetes mellitus. Extraction of the mandibular third molar was performed under local anesthesia: after the third molar surgery, two sutures were applied on the surgical site distally to the second mandibular molar: one single 3/0 silk stitch; one single 3/0 PTFE stitch. No sutures were applied on the release incision. Sutures were removed after 7 days and were immediately conserved and sent to the laboratory to be rated on the basis of colony-forming units (CFUs). CFUs were evaluated and reported on GraphPad Prism and transformed into its base 10 logarithm. Data were analyzed with a non-parametric Wilcoxon test, and p-values < 0.05 were evaluated as statistically significant. Results: All the patients attended the suture removal date, and all the sutures were present in the site. None of the surgical sites presented dehiscence. No stitch loss was reported, and no patient reported mouth washing or tooth brushing in the surgery site. All interventions were uneventful and no major complications were reported after M3M surgery. Bacterial retention resulted as statistically greater in silk sutures rather than PTFE sutures, both in Brain Heart Infusion samples (p = 0.003) and Wilkins-Chalgren anaerobe samples (p = 0.002). Conclusions: We found the PTFE suture to be superior to the silk suture in a reduction in the bacterial biofilm in both aerobic and anaerobic evaluations after M3M surgery

Evolution of production and forage quality in sown meadows of a mountain area inside Parmesan cheese consortium

Received: January 5th, 2021 ; Accepted: April 7th, 2021 ; Published: May 4th, 2021 ; Correspondence: [email protected] meadows, encompassing alfalfa and grass-legume mixtures, are the forage crops on which is based Parmesan cheese production system in the mountain area of the Apennines (central Italy). These grassland types experience, during their development, deep changes in terms of production potentiality, botanical composition and forage quality, thus these meadows have to be periodically renewed to guarantee adequate productive and qualitative performances. To have an accurate assessment of this evolution along time, a survey was performed in different mountain farms inside the Parmesan cheese consortium, analysing alfalfa meadows and grass-legumes mixtures of different ages. Grasslands were monitored during 2019, performing three samplings during growing season. Aboveground biomass production, botanical composition and crude protein content were collected during the survey. Results permitted to evaluate the level of production decrease along years, the evolution of analysed parameters among cutting dates and the dependence of productive and qualitative features on botanical composition and presence of sown species in the swards. Results were useful to hypothesize the composition of future mixtures, to improve management issues and to delineate the possible duration of sown meadows for the area with respect to different purposes in terms of desired productive or qualitative objectives

Evolution of productive and biodiversity features in lucerne fields of different ages

Received: January 10th, 2022 ; Accepted: February 23rd, 2022 ; Published: April 1st, 2022 ; Correspondence: [email protected] sativa is a legume forage crop characterized by high production of forage, with a notable nutritive value, but in mountain areas duration of the crop could be remarkably affected by severe environmental conditions. To assess the vegetation evolution of lucerne crops in relation to crop age, data from fields of lucerne of different ages were collected. The aim is the evaluation of lucerne productive performances, evolution of forage quality and assessment of recovery by autochthonous species that naturally recolonize the studied areas in relation to age of the cropped species. With increasing years, lucerne population was significantly decreased and replaced by different functional types of plants, such as perennial graminoids and short-lived forbs. Biodiversity increased significantly along time, and evolution of similarity indices demonstrated an evolution of vegetation toward that represented by reference grassland of the area. Productive characteristics of forage, in terms of aboveground biomass and quality, were negatively affected by age. Results permitted to assess the evolution of different features of lucerne for a mountain environment and to hypothesize the appropriate management for this resource, that could contemplate also the evolution towards the reconstitution of the reference habitat for the studied area

Research Notes : Italy : Light shock in soybean plantlets?

In a new soybean breeding program, 70 cross combinations were performed between the following varieties: \u27Agripro\u27, \u27Beeson\u27, \u27Caloria\u27, \u27Corsoy\u27, \u27Extra Early\u27, \u27Mikawashima\u27, \u27Norman\u27, \u27SRF150\u27, \u27SRF307 P\u27, \u27TXK505\u27, \u27TXK.535\u27, \u27Vansoy\u27, and \u27Wells\u27. Mother plants were the varieties indicated in Table l; in this table is also reported the number of combinations involv-ing each variety used as female and the number of seeds obtained

Upper Second Molar Distalization with Clear Aligners: A Finite Element Study

Among orthodontists and scientists, in the last years, upper molar distalization has been a debated topic in the orthodontic aligner field. However, despite that few clinical studies have been published, no insights on aligners' biomechanics regarding this movement are available. The aim of this study was to assess, through finite element analysis, the force system resulting in the upper arch during second maxillary molar distalization with clear aligners and variable attachments settings. The average tooth distalization was found to be 0.029, with buccal flaring of the upper incisors in all attachment configurations. The mesial deformation of the aligner was registered to be 0.2 mm on average. Different pressure areas on the interface between aligners and upper molars were registered, with the mesial attachment surface to be directly involved when present. Periodontal ligament pressure was reported to range between 67 g/cm(2) and 132 g/cm(2). Configurations with rectangular attachments from second molar-to-canine and from first molar-to-canine present, in an in silico environment, almost equal efficiency in distalizing the upper second molar. However, attachments from the second molar to the canine are suggested to be adopted in clinical environments due to greater feasibility in everyday practice

Adherence to Mediterranean diet, physical activity level, and severity of periodontitis: Results from a university-based cross-sectional study

Background: The aim of this study was to evaluate the association between adherence to Mediterranean diet (MD) and physical activity (PA) level with the periodontal status of a University-based cohort of individuals. Methods: A total of 235 individuals were included in the study. MD adherence and PA level were registered through validated questionnaires, together with a full periodontal examination. Crude and adjusted odds ratios (ORs) [95% confidence interval] were calculated to evaluate the association between MD adherence, PA level, and periodontitis severity. A final logistic multivariate regression model was built to evaluate the impact of the combination between low MD adherence and low PA level on the prevalence of Stage III/IV periodontitis. Results: The adjusted ORs for Stage III/IV periodontitis were 1.65 [0.84 to 3.28; P = 0.42] for low PA and 5.63 [3.21 to 9.84; P = 0.00] for low MD adherence. The final logistic multivariate regression model resulted in OR = 10.23 [4.01, 26.09; P = 0.00] of having Stage III/IV periodontitis in individuals with low MD adherence and low PA. Conclusions: Individuals conducting a lifestyle characterized by the combination of low MD adherence and lack of regular exercise had 10 times the odds to have severe forms of periodontitis. © 2022 The Author