Insights to Problems, Research Trend and Progress in Techniques of Sentiment Analysis

The research-based implementations towards Sentiment analyses are about a decade old and have introduced many significant algorithms, techniques, and framework towards enhancing its performance. The applicability of sentiment analysis towards business and the political survey is quite immense. However, we strongly feel that existing progress in research towards Sentiment Analysis is not at par with the demand of massively increasing dynamic data over the pervasive environment. The degree of problems associated with opinion mining over such forms of data has been less addressed, and still, it leaves the certain major scope of research. This paper will brief about existing research trends, some important research implementation in recent times, and exploring some major open issues about sentiment analysis. We believe that this manuscript will give a progress report with the snapshot of effectiveness borne by the research techniques towards sentiment analysis to further assist the upcoming researcher to identify and pave their research work in a perfect direction towards considering research gap

A report of the threatened plant <i>Decalepis hamiltonii</i> Wight & Arn. (Asclepiadaceae) from the mid elevation forests of Pachamalai Hills of the Eastern Ghats, Tamil Nadu, India

The Pachamalai Hills are a part of the Eastern Ghats and are situated in the central region of Tamil Nadu. The vegetated area is distributed into 35 reserved forests of Pachamalai Hills. The plant Decalepis hamiltonii Wight & Arn. is one of the threatened plant found in the study area. This indicates that the Pachamalai Hills can harbour good vegetation which are the vestiges of a luxuriant vegetation cover of the past era, hence, need to be protected. The hills are most significant socio-culturally because of the diversified forest patches found there. These hills have been studied earlier mainly for floristic analysis. Before this, D. hamiltonii has not been collected from Pachamalai

Novel mutations in PRG4 gene in two Indian families with camptodactyly-arthropathy- coxa vara- pericarditis (CACP) syndrome

Background & objectives: Camptodactyly - arthropathy- coxa vara- pericarditis (CACP) syndrome is an autosomal recessive disorder caused by mutations in the PRG4 (proteoglycan 4) gene. Hallmarks of the syndrome include congenital or early-onset camptodactyly and arthropathy with synovial hyperplasia, progressive coxa vara deformity and non-inflammatory pericardial effusions. Till date only around 25 pathogenic mutations have been reported in this gene and none have been reported from India. We report here the mutations in the PRG4 gene in three patients of CACP from two unrelated families from India. Methods: Molecular genetic studies were done for the three patients with the CACP syndrome, from two unrelated Indian families, through sequence analysis of all coding exons and the exon-intron boundaries of the PRG4 gene. Results: Two novel frame-shift deletion mutations leading to premature protein termination were found. One patient was identified to be homozygous for a 2 base pair deletion in exon 6 (c.2645_2646delGA) and the two affected siblings from the other family were found to be homozygous for a 4 base pair deletion in exon 6 (c.2883_2886delAAGA). Conclusions: This is perhaps the first report of PRG4 mutations from India. Further mutation studies in Indian CACP cases will help to determine the mutation spectrum of the PRG4 gene in the Indian population and also help to further elucidate the molecular pathology and the genotype-phenotype correlation of this rare disease

Dual transcripts of BCR-ABL & different polymorphisms in chronic myeloid leukaemia patients

Background & objectives: Chronic myeloid leukaemia is (CML) characterized by the presence of a hallmark chromosomal translocation, the Philadelphia chromosome. Although there are many reports available regarding the different variants of BCR-ABL in CML, we studied the co-expression of e13a2 and e14a2 transcripts and a few polymorphisms in CML patients. Methods: Molecular genetics approach was adapted to screen for polymorphisms, mutation and translocation in BCR, ABL kinase domain and BCR-ABL breakpoint region in 73 CML patients. Results: All eight patients with dual transcripts were found to harbour an exonic polymorphism (c.2700 T>C) and an intronic polymorphism (g.109366A>G) that were earlier reported to be associated with co-expression of both the transcripts. We also observed c.763G>A mutation in ABL kinase domain and two polymorphisms, c.2387 A>G and c.2736A>G in the BCR gene. Interpretation & conclusions: Though our data support the previous findings that co-expression of BCR-ABL transcripts is due to the occurrence of exonic and intronic polymorphisms in the BCR gene, it also shows that the intronic polymorphism can arise without the linked exonic polymorphism. The occurrence of ABL kinase domain mutation is less frequent in Indian population