Additional file 1: Figure S1. of The Z-cad dual fluorescent sensor detects dynamic changes between the epithelial and mesenchymal cellular states

miR-200 sensor construct and validation; Z-cad sensor validation. A) Five miR-200 family binding sites were placed downstream of d2GFP in the FUGW lentiviral expression vector. B) Fluorescent microscopy of T11 cells containing the Z-cad dual sensor after 4 days of doxycycline treatment. C) Flow cytometry analysis of d2GFP-200 or control d2GFP expression and Ecad-RFP upon miR-200c/141 induction after 4 days of 2 μg/mL doxycycline treatment in the indicated cell lines (n = 3 biological replicates per group). D) Flow cytometry analysis of BLSL12 breast cancer cells containing the indicated sensors. miR-200c/141 was induced with 2 μg/mL doxycycline for 4 days. E) Fluorescent confocal microscopy of BLSL12 cells containing Z-cad dual sensor after 4 days of doxycycline treatment. Scale bar = 20 μm. (TIF 4770 kb

Additional file 6: Figure S6. of The Z-cad dual fluorescent sensor detects dynamic changes between the epithelial and mesenchymal cellular states

Z-cad population gates and their CD24/CD44 profiles. A) Flow cytometry analysis was used to gate HMLER cells based on the indicated Z-cad expression profiles (in red boxes). Gated cells from each population were subsequently analyzed for CD24 and CD44 expression as shown in B). (TIF 1218 kb