24 research outputs found
Arabidopsis protein phosphatase DBP1 nucleates a protein network with a role in regulating plant defense
Arabidopsis thaliana DBP1 belongs to the plant-specific family of DNA-binding protein phosphatases. Although recently
identified as a novel host factor mediating susceptibility to potyvirus, little is known about DBP1 targets and partners and
the molecular mechanisms underlying its function. Analyzing changes in the phosphoproteome of a loss-of-function dbp1
mutant enabled the identification of 14-3-3l isoform (GRF6), a previously reported DBP1 interactor, and MAP kinase (MAPK)
MPK11 as components of a small protein network nucleated by DBP1, in which GRF6 stability is modulated by MPK11
through phosphorylation, while DBP1 in turn negatively regulates MPK11 activity. Interestingly, grf6 and mpk11 loss-offunction
mutants showed altered response to infection by the potyvirus Plum pox virus (PPV), and the described molecular
mechanism controlling GRF6 stability was recapitulated upon PPV infection. These results not only contribute to a better
knowledge of the biology of DBP factors, but also of MAPK signalling in plants, with the identification of GRF6 as a likely
MPK11 substrate and of DBP1 as a protein phosphatase regulating MPK11 activity, and unveils the implication of this
protein module in the response to PPV infection in Arabidopsis.This work was supported by the Spanish MICINN (Grants BFU2009-09771, EUI2009-04009 to PV), Generalitat Valenciana (Prometeo2010/020 to PV) and the German DFG (SCHE 235/15-1 to DS). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Carrasco Jiménez, JL.; Castelló Llopis, MJ.; Naumann, K.; Lassowskat, I.; Navarrete Gomez, ML.; Scheel, D.; Vera Vera, P. (2014). Arabidopsis protein phosphatase DBP1 nucleates a protein network with a role in regulating plant defense. PLoS ONE. 9:1-10. https://doi.org/10.1371/journal.pone.0090734S1109Carrasco, J. L. (2003). A novel transcription factor involved in plant defense endowed with protein phosphatase activity. The EMBO Journal, 22(13), 3376-3384. doi:10.1093/emboj/cdg323Carrasco, J. L., Ancillo, G., Castelló, M. J., & Vera, P. (2005). 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Stress signaling in plants: a mitogen-activated protein kinase pathway is activated by cold and drought.
A MAP kinase is activated late in plant mitosis and becomes localized to the plane of cell division
In eukaryotes, mitogen-activated protein kinases (MAPKs) are part of signaling modules that transmit diverse stimuli, such as mitogens, developmental cues, or various stresses. Here, we report a novel alfalfa MAPK, Medicago MAP kinase 3 (MMK3). Using an MMK3-specific antibody, we detected the MMK3 protein and its associated activity only in dividing cells. The MMK3 protein could be found during all stages of the cell cycle, but its protein kinase activity was transient in mitosis and correlated with the timing of phragmoplast formation. Depolymerization of microtubules by short treatments with the drug amiprophosmethyl during anaphase and telophase abolished MMK3 activity, indicating that intact microtubules are required for MMK3 activation. During anaphase, MMK3 was found to be concentrated in between the segregating chromosomes; later, it localized at the midplane of cell division in the phragmoplast. As the phragmoplast microtubules were redistributed from the center to the periphery during telophase, MMK3 still localized to the whole plane of division; thus, phragmoplast microtubules are not required to keep MMK3 at this location. Together, these data strongly support a role for MMK3 in the regulation of plant cytokinesis