Number of Arabidopsis <i>DEFL</i> expression by tissue and condition

*<p>Some DEFLs were expressed in more than one organ and/or condition, so, the number of expressed DEFLs in each organ/condition does not add up to the total.</p

Gene expression profiles of Arabidopsis.

<p>A, <i>SCR</i>s. B, <i>MEG</i>s. C, Seeding- and root-specific/enhanced <i>DEFL</i>s. Treatments and heatmap colors are as depicted in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0058992#pone-0058992-g004" target="_blank">Figure 4</a>.</p

Venn diagrams of expressed Arabidopsis <i>DEFL</i>s in different treatments.

<p>A, Number of expressed <i>DEFL</i>s and <i>MEG</i>s in various tissues. B, Comparison of different gene discovery approaches for the number of identified expressed <i>DEFL</i>s by each technique.</p

Transcriptional fusion analysis of Arabidopsis <i>DEFL</i> promoter constructs in T4 transgenic seedlings expressing pSCR:<i>LhG4</i>/<i>OP</i>:GUS.

<p>Histochemical staining for GUS activity in 1-week-old seedlings transformed with promoter constructs of Arabidopsis <i>DEFLs</i>. A, At1g60989. B, At2g26020. C, At5g60553. D, At3g63360. E, At4g30074. F, At5g05598. Scale bar 10 µm.</p

Scatter plots showing correlation of SBQ normalized AtMtDEFL array expression values compared to publicly available GEO ATH1 array data.

<p>A, roots. B, leaves. C, inflorescences. Probe sets for 171 invariants and 91 marker genes are identical in AtMtDEFL and ATH1 arrays. The 37 <i>DEFL</i> probe sets were represented with different probes on the two arrays with differences in some cases in the underlying gene model. Log₂ signals for biological replicates were averaged prior to cross-platform comparison. GEO accessions for ATH1 arrays included: GSM131558, GSM131559, GSM131560 (roots); GSM131498, GSM131499, GSM131500 (leaves); and GSM62694, GSM227612 (inflorescences).</p

Venn diagrams of Medicago <i>DEFL</i>s expressed in different treatments.

<p>A, Comparison among all tissue types. B, Tissues inoculated with microbial symbionts and plant pathogens. C, Reproductive tissues.</p

Transcript profiles of expressed Medicago <i>DEFLs</i>.

<p>Treatments were: (1) Germinating seed, (2) stem, (3) flower buds, (4) flowers, (5) seed, (6) uninoculated root for mycorrhizal control, (7) mycorrhizal root, (8) uninoculated root for nodule control, (9) nodules at 14 dpi, (10) <i>P. medicaginis</i> mock-inoculated root, (11) <i>P. medicaginis</i>-inoculated root, (12) <i>C. trifolii</i> mock-inoculated leaf, and (13) <i>C. trifolii</i>-inoculated leaf. The heat map shows median scaling of SBQ normalized signal intensity values as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0058992#s5" target="_blank">materials and methods</a> and colors represent high transcript abundance as depicted in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0058992#pone-0058992-g004" target="_blank">Figure 4</a>.</p