Effect of Penetration Enhancer DMSO on In-Vitro Skin Permeation of Acyclovir Transdermal Microemulsion Formulation

The aim of this research was to enhance the flux of transdermal drug delivery by using penetration enhancers DMSO. Skin penetration enhancers have been used to improve bioavailability and increase the range of drugs for which topical and transdermal delivery is a viable option which penetrate into skin to reversibly decrease the barrier resistance. Penetration enhancing activity of dimethylsulphoxide (DMSO) at 5% w/w and 10% w/w concentration were determined in aqueous solution of ACV and in microemulsion formulations though calculation of transdermal flux of ACV with Keshary Chein Frenz Diffusion cell by using wistar albino rat skin. The transdermal flux of formulations PD, PD5D, PD10D, ME1 and ME10D was found to be 2.47, 50.7529, 119.7691, 238.1432 and 266.6721μg/cm2/h. The flux of microemulsion formulation ME10D was found 266.6721± 8.49 μg/cm2/h. Which showed highest value and skin flux of the drug could be enhanced up to 107 fold compared to its aqueous solution by preparing microemulsion ME10D. DMSO in microemulsion formulation is safe to the skin at 10% DMSO w/w.Keywords: DMSO, Penetration enhancer, Ethanol, Transdermal Microemulsion, Acyclovir (ACV

Effect of Penetration Enhancer DMSO on In-Vitro Skin Permeation of Acyclovir Transdermal Microemulsion Formulation

The aim of this research was to enhance the flux of transdermal drug delivery by using penetration enhancers DMSO. Skin penetration enhancers have been used to improve bioavailability and increase the range of drugs for which topical and transdermal delivery is a viable option which penetrate into skin to reversibly decrease the barrier resistance. Penetration enhancing activity of dimethylsulphoxide (DMSO) at 5% w/w and 10% w/w concentration were determined in aqueous solution of ACV and in microemulsion formulations though calculation of transdermal flux of ACV with Keshary Chein Frenz Diffusion cell by using wistar albino rat skin. The transdermal flux of formulations PD, PD5D, PD10D, ME1 and ME10D was found to be 2.47, 50.7529, 119.7691, 238.1432 and 266.6721μg/cm2/h. The flux of microemulsion formulation ME10D was found 266.6721± 8.49 μg/cm2/h. Which showed highest value and skin flux of the drug could be enhanced up to 107 fold compared to its aqueous solution by preparing microemulsion ME10D. DMSO in microemulsion formulation is safe to the skin at 10% DMSO w/w.Keywords: DMSO, Penetration enhancer, Ethanol, Transdermal Microemulsion, Acyclovir (ACV

Laser induced elastico mechano luminescence of SrAl2O4 : Eu phosphor

When γ – irradiated elastic mechano luminescent materials SrAl2O4 : Eu are exposed to 1060 nm infrared pulse of nanosecond duration from CO2 laser, then stress produced in the crystals excites visible luminescence due to piezoelectric field in SrAl2O4 : Eu phosphor because they are non – centro symmetric.  In the present investigation SrAl2O4 : Eu crystals are given laser shocks and ML intensity recorded. During laser induced shocks, ML intensity increases linearly with stress and attains a peak value at a particular time and then decays exponentially with time. A theoretical approach has been proposed to explain the experimental results.&nbsp

Utilisation of sea nodules leaching residue for adsorption of Ni(II) ions

Polymetallic sea nodules may be considered as lean grade ore of Cu, Ni & Co. After recovery of these valuable metals, a huge quantity of residue (~70% of ore body) is generated. In the present paper, investigations carried out for the application of leached sea nodule residue for the removal of Ni(II) from aqueous solution by adsorption, are described. Several parameters have been varied to study the feasibility of using residue as potential adsorbent for remediation Ni(II) contaminated water. The adsorption kinetics followed pseudo first-order equation and the rate of adsorption increased with solution temperature. Kinetics data of Ni(II) adsorption was also discussed using diffusion models of Webber-Morris and Dumwald-Wagner models. The equilibrium data was best fitted into Langmuir adsorption isotherm and the maximum adsorption capacities was found to be 15.15 mg g-1 at pH 5.5 and temperature 303 K, which decreased to 10.64 mg g-1 upon raising the solution temperature to 323 K. The activation energy for Ni(II) adsorption onto leached sea nodule residue was 9.56 kJ mol−1 indicated physical sorption. Desorption studies showed successful regeneration of adsorbent and recovery of Ni. This process can be utilised for removal and recovery of Ni from the industrial effluent

Quality control parameters of Gandhakadi Yoga tablets W. S. R. To its microscopic evaluation

Gandhakadi Yoga is a formulation suggested for Loha Sevanajanya Vikara Prashamana (Iron overload) in Ayurveda Prakasha and can be used as iron chelator which might help to decrease the iron overload. The present study was carried out to standardize the finished product Gandhakadi Yoga tablets for confirmation of its identity, quality and purity. Thus a trial was made to establish the Quality assessing parameters for Gandhakadi Yoga tablets by simple microscopic technique. The characters i.e. black debris of Gandhaka treated with Bhringaraja, light yellow brown content of Gandhaka, fragment of spongy parenchyma of Agastya leaf, oil globules and epicarp cells with tannin content of Vidanga etc. were the characteristic features of the finished product

DEVELOPMENT AND VALIDATION OF UV SPECTROMETRIC METHOD FOR QUANTITATIVE DETERMINATION OF ULIPRISTAL ACETATE

Objective: To develop and validate UV spectrometric method for quantitative determination of ulipristal acetate.Methods: The solvent selected was methanol and detection was carried out at 302 nm.Results: Linearity of the proposed method was found to be between 5–20 μg/ml. LOD and LOQ were found to be 0.0062 μg/ml and 0.0187 μg/ml, respectively. The % recovery of the proposed method was found to be 98.83 %-100.32 %. The method was found to be precise as the values of % RSD obtained for both intraday and interday, precision studies were found to be<2.0 %. The method was robust and can be useful for routine analysis of formulations containing ulipristal acetate.Conclusion: The developed method was found to be simple, sensitive, linear, accurate, precise and robust. The developed and validated method can be used for quantitative determination of ulipristal acetate in bulk drugs and dosage form.Â

CHARACTERIZATION & EVALUATION OF ANTIBACTERIAL, ANTIFUNGAL ACTIVITY OF ENVIRONMENT FRIENDLY CAPPARIS DECIDUA MICROEMULSION

Objective: The present study was undertaken with the objective to develop microemulsion from the ethanolic extract of the plant, Capparis decidua and evaluate its potency against microorganisms (bacteria & fungi).Methods: The solubility of the extract was tested in various solvents to determine the oil phase to be used in the microemulsion system. Microemulsion formulations were developed from the plant extract and their physico-chemical studies were carried out as per standard parameter.Results: The prepared microemulsion was tested for its antimicrobial and antifungal activities. Preliminary screening of the microemulsion showed the potent antimicrobial and antifungal activity. The development of microemulsion was confirmed by Transmission Electron Microscopic (TEM) analysis.Conclusion: 5% (w/w) microemulsion from the ethanolic extract of the Capparis decidua was successfully prepared. The microemulsion was found to possess potent antibacterial and antifungal activities.Â