Limb salvage after delayed arterial repair in compound Grade III C fracture humerus: a case report

A combination of brachial artery injury and fracture shaft of humerus is a rare phenomenon. There is a general apprehension regarding survival of a limb after vascular injury. Only few studies exist in literature that discuss about the survival of such limbs. Our case is a 56 years old female patient who presented three hours after sustaining injury in the form of fracture shaft of humerus and complete transection of brachial artery distal to the origin of profunda brachii. Even though Doppler USG done initially revealed flow in the vessels distal to the injury, a CT Angiogram done later revealed cutting of the brachial artery. This prompted us to perform brachial artery exploration and repair. post operatively, digital subtraction angiography showed absence of flow in the brachial artery but limb survived due to extensive collateral circulation. Even after the golden period of vascular repair has lapsed, arterial repair is recommended if there is no evidence of gangrene. This should be supplemented with adequate systemic anticoagulants and/or fasciotomy

QTL and QTL × Environment Effects on Agronomic and Nitrogen Acquisition Traits in Rice

Agricultural environments deteriorate due to excess nitrogen application. Breeding for low nitrogen responsive genotypes can reduce soil nitrogen input. Rice genotypes respond variably to soil available nitrogen. The present study attempted quantification of genotype × nitrogen level interaction and mapping of quantitative trait loci (QTLs) associated with nitrogen use efficiency (NUE) and other associated agronomic traits. Twelve parameters were observed across a set of 82 double haploid (DH) lines derived from IR64/Azucena. Three nitrogen regimes namely, native (0 kg/ha; no nitrogen applied), optimum (100 kg/ha) and high (200 kg/ha) replicated thrice were the environments. The parents and DH lines were significantly varying for all traits under different nitrogen regimes. All traits except plant height recorded significant genotype×environment interaction. Individual plant yield was positively correlated with nitrogen use efficiency and nitrogen uptake. Sixteen QTLs were detected by composite interval mapping. Eleven QTLs showed significant QTL×environment interactions. On chromosome 3, seven QTLs were detected associated with nitrogen use, plant yield and associated traits. A QTL region between markers RZ678, RZ574 and RZ284 was associated with nitrogen use and yield. This chromosomal region was enriched with expressed gene sequences of known key nitrogen assimilation genes

Mixed Layer Budget Terms on Acoustic Propagation A Study based on the Butterfly Track Experiment in the South Eastern Arabian Sea

A butterfly type of repeat track cruise was carried out in the South Eastern Arabian Sea (off Minicoy) onboard INS Sagardhwani during July 2016 to Aug 2016. We have also made use of the data from OMNI buoy, AD09, which is about 6 km close to the centre station of butterfly track. Air sea flux, the horizontal current data from AD09 and the time series data collected from the butterfly experiment were analyzed to compute the mixed layer heat and salt budget. The short-term thermo-haline variability off Minicoy, relative contribution of heat/salt budget terms in MLD and its effects on acoustic propagation are addressed in this paper. In this study, we found that most dominating term in the mixed layer heat budget estimation is net surface heat flux followed by the advective terms. However the salinity in the mixed layer is dominated by the contribution of buoyancy mixing due to night time evaporative cooling. During the calm, sunny day, the so-called afternoon effect due to the diurnal heating restricts the sonar range. But during the windy day, the wind/wave mixing prevents the warming of the surface layer which in turn enhances the sonar range. Similarly, the night time cooling also enhance the acoustic propagation range. The presence of Arabian Sea High Salinity Watermass in the surface layer also enhances the acoustic propagation

Genetic variability studies in forage type hybrid parents of pearl millet

One hundred and sixteen forage-type hybrid parents of pearl millet were investigated in summer season for two years, the results revealed that the traits like number of tillers and leaf to stem ratio (L/S) at first cut (50 days after planting); and dry forage yield (DFY) at second cut (30 days after first cut) had high phenotypic coefficient of variation (PCV) values than genotypic coefficient of variation (GCV), hence selection will not be effective for these traits. Many of the desirable and undesirable forage quantity and quality linked traits under investigation had GCV almost equivalent to PCV, thus selection to improve those traits might be effective. High to moderate heritability coupled with high genetic advance per cent of mean (GA) was observed for plant height in both the cuts, indicating, this trait to be controlled by additive gene action and thus can be improved through selection, while, moderate heritability and high GA was observed for DFY at first cut; for green forage yield (GFY) at second cut and for total green forage yield (TGFY) from both the cuts, hence improvement in these traits will be possible through selection at later generations

Correlation and Path Coefficient Analysis of Biomass Yield and Quality Traits in Forage Type Hybrid Parents of Pearl Millet

One hundred and sixteen forage type hybrid parents (seed and pollinator parents) were investigated at International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), India, in summer seasons of 2015 and 2016 for estimating inter-relationships between forage quantity and quality traits and their direct and indirect effects. Significant negative correlation but of low value was found between crude protein (CP), In vitro organic matter digestibility (IVOMD) and dry forage yield (DFY). The direct and indirect effect of these forage quantity related traits on the DFY and other forage quality related traits on IVOMD was investigated in pearl millet. Path analysis revealed that total green forage yield (TGFY) had highest positive direct effect on DFY for forage quantity traits followed by plant height (PH). For forage quality traits, metabolizable energy (ME) had highest direct effect on IVOMD in both the cuts. CP and cellulose also had positive direct effects on IVOMD in both the cuts

Circulating tumor cells in blood of primary breast cancer patients assessed by a novel RT-PCR test kit and comparison with status of bone marrow-disseminated tumor cells

In breast cancer, circulating tumor cells (CTCs)/disseminated tumor cells (DTCs) may serve as independent adverse prognostic variables, to monitor the course of the disease and to predict response or failure to cancer therapy. Most of the techniques to enumerate DTCs in the bone marrow or CTCs in the bloodstream of breast cancer patients rely on a combination of an enrichment step and a detection step. A novel RT-PCR method, the AdnaTest BreastCancer™ kit, was developed for the enrichment of CTCs from peripheral blood of breast cancer patients followed by identification of CTC-associated marker transcripts by reverse transcription and PCR. Although this test has been demonstrated to identify breast cancer patients at risk, standardization of this technique and direct comparison with other established breast cancer CTC enrichment and detection techniques is still lacking, but highly needed. This is done best within prospective clinical trials, such as in the ongoing DETECT, SUCCESS, and BR-01-2004 trials

Social deprivation and exposure to health promotion. A study of the distribution of health promotion resources to schools in England

This article has been made available through the Brunel Open Access Publishing Fund and is available from the specified link - Copyright @ 2010 Chivu and ReidpathBACKGROUND: Area deprivation is a known determinant of health. It is also known that area deprivation is associated with lower impact health promotion. It is less well known, however, whether deprived areas are less responsive to health promotion, or whether they are less exposed. Using data from a national, school-based campaign to promote vaccination against the human papilloma virus (HPV), the relationship between area deprivation and exposure was examined. METHODS: Taking advantage of a health promotion campaign to provide information to schools about HPV vaccination, a cross sectional study was conducted to examine the relationship between area level, social deprivation, and take-up of (i.e., exposure to) available health promotion material. The sample was 4,750 schools across England, including government maintained and independent schools. The relationship between area deprivation and exposure was examined using bi- and multivariate logistic regression. RESULTS: It was found that schools in the least deprived quintile had 1.32 times the odds of requesting health promotion materials than schools in the most deprived areas (p = .01). This effect was independent of the school size, the type of school, and the geographic region. Conclusion The relationship between area deprivation and the impact of health promotion may be due, at least in part, to differential levels of exposure. The study was limited in scope, pointing to the need for more research, but also points to potentially important policy implications

Pericentromeric satellite repeat expansions through RNA-derived DNA intermediates in cancer

Aberrant transcription of the pericentromeric human satellite II (HSATII) repeat is present in a wide variety of epithelial cancers. In deriving experimental systems to study its deregulation, we observed that HSATII expression is induced in colon cancer cells cultured as xenografts or under nonadherent conditions in vitro, but it is rapidly lost in standard 2D cultures. Unexpectedly, physiological induction of endogenous HSATII RNA, as well as introduction of synthetic HSATII transcripts, generated cDNA intermediates in the form of DNA/RNA hybrids. Single molecule sequencing of tumor xenografts showed that HSATII RNA-derived DNA (rdDNA) molecules are stably incorporated within pericentromeric loci. Suppression of RT activity using small molecule inhibitors reduced HSATII copy gain. Analysis of whole-genome sequencing data revealed that HSATII copy number gain is a common feature in primary human colon tumors and is associated with a lower overall survival. Together, our observations suggest that cancer-associated derepression of specific repetitive sequences can promote their RNA-driven genomic expansion, with potential implications on pericentromeric architecture

A Magnetically Torqued Disk Model for Be Stars

Despite extensive study, the mechanisms by which Be star disks acquire high densities and angular momentum while displaying variability on many time scales are still far from clear. In this paper, we discuss how magnetic torquing may help explain disk formation with the observed quasi-Keplerian (as opposed to expanding) velocity structure and their variability. We focus on the effects of the rapid rotation of Be stars, considering the regime where centrifugal forces provide the dominant radial support of the disk material. Using a kinematic description of the angular velocity, vphi(r), in the disk and a parametric model of an aligned field with a strength B(r) we develop analytic expressions for the disk properties that allow us to estimate the stellar surface field strength necessary to create such a disk for a range of stars on the main-sequence. The model explains why disks are most common for main-sequence stars at about spectral class B2 V. The earlier type stars with very fast and high density winds would require unacceptably strong surface fields (> 10^3 Gauss) to form torqued disks, while the late B stars (with their low mass loss rates) tend to form disks that produce only small fluxes in the dominant Be diagnostics. For stars at B2 V the average surface field required is about 300 Gauss. The predicted disks provide an intrinsic polarization and a flux at Halpha comparable to observations. We also discuss whether the effect on field containment of the time dependent accumulation of matter in the flux tubes/disk can help explain some of the observed variability of Be star disks.Comment: ApJ, in press. 46 pages, 12 figure