Postnatal Developmental Trends in Membrane Excitability and BK Channel Function in the Rodent Hippocampus

This dissertation outlines the postnatal development of excitability as well as expression and function of BK potassium channels in hippocampal neurons. I used patch clamp electrophysiology to measure how neuronal action potential waveforms and action potential firing frequencies change in early development, and how pharmacological blockade of BK channels affects these properties in hippocampal neurons. I also describe how the protein expression of the BK channel pore-forming α subunit and mRNA expression of different variants of the pore forming α subunit and auxiliary beta-4 subunit changes with development. I demonstrate in both cultured rat hippocampal neurons across the first seven postnatal days and in putative mouse hippocampal pyramidal neurons from postnatal day four to fifteen in acutely prepared slices that maturation brings changes in action potential kinetics and large increases in the capacity for high frequency firing. In the cultured neurons I demonstrate that during the first postnatal week, the contribution of BK channels to action potential repolarization decreases but the channel’s role in maintaining high-frequency firing increases. This change in the timing of BK channel activity in the action potential waveform is accompanied by a slowing of BK current onset measured by a decreasing effect of BK current blockade on potassium current rise time. Additionally, I demonstrated in pyramidal neurons in mouse brain slices that there is a loss of BK channel contribution to action potential repolarization between postnatal day four and fifteen. These changes in the BK channel’s role in neuronal action potential firing are accompanied by large increases in the expression of α subunit protein measured by Western blot and by large increases in mRNA transcript expression, measured by RT-qPCR, of both the α and beta-4 subunits in the hippocampus. The rise in beta-4 subunit expression can explain the apparent slowing of BK channel activation through development. I investigated whether BK channel blockade in neonatal brain slices could attenuate hyperexcitability in a model of seizure activity as BK channels in immature neurons share properties of BK channel variants implicated in epilepsy but found no evidence to support this hypothesis

Hubble Space Telescope Images of Stephan's Quintet: Star Cluster Formation in a Compact Group Environment

Analysis of Hubble Space Telescope/Wide Field Planetary Camera 2 images of Stephan's Quintet, Hickson Compact Group 92, yielded 115 candidate star clusters (with V-I < 1.5). Unlike in merger remants, the cluster candidates in Stephan's Quintet are not clustered in the inner regions of the galaxies; they are spread over the debris and surrounding area. Specifically, these sources are located in the long sweeping tail and spiral arms of NGC 7319, in the tidal debris of NGC 7318B/A, and in the intragroup starburst region north of these galaxies. Analysis of the colors of the clusters indicates several distinct epochs of star formation that appear to trace the complex history of dynamical interactions in this compact group.Comment: 24 pages, 21 figures (13 PostScript and 8 JPEG), LaTeX (uses aastexug.sty), accepted for publication in the Astronomical Journal (July 2001). Full-resolution PostScript figures available at http://www.astro.psu.edu/users/gallsc/sq/figs.tar.g

The Luminosity Function of Galaxies in Compact Groups

From R-band images of 39 Hickson compact groups (HCGs), we use galaxy counts to determine a luminosity function extending to M_R=-14.0, approximately two magnitudes deeper than previous compact group luminosity functions. We find that a single Schechter function is a poor fit to the data, so we fit a composite function consisting of separate Schechter functions for the bright and faint galaxies. The bright end is best fit with M^*=-21.6 and alpha=-0.52 and the faint end with M^*=-16.1 and alpha=-1.17. The decreasing bright end slope implies a deficit of intermediate luminosity galaxies in our sample of HCGs and the faint end slope is slightly steeper than that reported for earlier HCG luminosity functions. Furthermore, luminosity functions of subsets of our sample reveal more substantial dwarf populations for groups with x-ray halos, groups with tidal dwarf candidates, and groups with a dominant elliptical or lenticular galaxy. Collectively, these results support the hypothesis that within compact groups, the initial dwarf galaxy population is replenished by "subsequent generations" formed in the tidal debris of giant galaxy interactions.Comment: 26 pages, to be published in The Astrophysical Journal, 8 greyscale plates (figures 1 and 2) can be retrieved at http://www.astro.psu.edu/users/sdh/pubs.htm

Prompt optical observations of GRB050319 with the Swift UVOT

The UVOT telescope on the Swift observatory has detected optical afterglow emission from GRB 050319. The flux declines with a power law slope of alpha = -0.57 between the start of observations some 230 seconds after the burst onset (90s after the burst trigger) until it faded below the sensitivity threshold of the instrument after ~5 x 10^4s. There is no evidence for the rapidly declining component in the early light curve that is seen at the same time in the X-ray band. The afterglow is not detected in UVOT shortward of the B-band, suggesting a redshift of about 3.5. The optical V-band emission lies on the extension of the X-ray spectrum, with an optical to X-ray slope of beta = -0.8. The relatively flat decay rate of the burst suggests that the central engine continues to inject energy into the fireball for as long as a few x 10^4s after the burst.Comment: 16 pages, 4 figures, accepted by Ap

From Globular Clusters to Tidal Dwarfs: Structure Formation in the Tidal Tails of Merging Galaxies

Using V and I images obtained with WFPC2/HST, we investigate compact stellar structures within tidal tails. Six regions of tidal debris in the four classic ``Toomre Sequence'' mergers: NGC 4038/39 (``Antennae''), NGC 3256, NGC 3921, and NGC 7252 (``Atoms for Peace'') have been studied in order to explore how the star formation depends upon the local and global physical conditions. These mergers sample a range of stages in the evolutionary sequence and tails with and without embedded tidal dwarf galaxies. The six tails are found to contain a variety of stellar structures, with sizes ranging from those of globular clusters up to those of dwarf galaxies. From V and I WFPC2 images, we measure the luminosities and colors of the star clusters. NGC 3256 is found to have a large population of blue clusters (0.2 < V-I < 0.9), particularly in its Western tail, similar to those found in the inner region of the merger. In contrast, NGC 4038/39 has no clusters in the observed region of the tail, only less luminous point sources likely to be individual stars. A significant cluster population is clearly associated with the prominent tidal dwarf candidates in the eastern and western tails of NGC 7252. The cluster-rich Western tail of NGC 3256 is not distinguished from the others by its dynamical age or by its total HI mass. However, the mergers that have few clusters in the tail all have tidal dwarf galaxies, while NGC 3256 does not have prominent tidal dwarfs. We speculate that star formation in tidal tails may manifest itself either in small structures like clusters along the tail or in large structures such as dwarf galaxies, but not in both. Also, NGC 3256 has the highest star formation rate of the four mergers studied, which may contribute to the high number of star clusters in its tidal tails.Comment: Accepted to Astronomical Journal. 34 pages including 15 figures and 4 table

Ultraviolet, Optical, and X-Ray Observations of the Type Ia Supernova 2005am with Swift

We present ultraviolet and optical light curves in six broadband filters and grism spectra obtained by Swift's Ultraviolet/Optical Telescope for the Type Ia supernova SN2005am. The data were collected beginning about four days before the B-band maximum, with excellent coverage of the rapid decline phase and later observations extending out to 69 days after the peak. The optical and near UV light curve match well those of SN1992A. The other UV observations constitute the first set of light curves shorter than 2500 Angstroms and allow us to compare the light curve evolution in three UV bands. The UV behavior of this and other low redshift supernovae can be used to constrain theories of progenitor evolution or to interpret optical light curves of high redshift supernovae. Using Swift's X-Ray Telescope, we also report the upper limit to SN2005am's X-ray luminosity to be 1.77 x 10^40 erg s^-1 in the 0.3--10 keV range from 58,117 s of exposure time.Comment: 15 pages, including 3 figures and 2 tables, submitted to Astrophysical Journa

Swift-UVOT detection of GRB 050318

We present observations of GRB 050318 by the Ultra-Violet and Optical Telescope (UVOT) on-board the Swift observatory. The data are the first detections of a Gamma Ray Burst (GRB) afterglow decay by the UVOT instrument, launched specifically to open a new window on these transient sources. We showcase UVOTs ability to provide multi-color photometry and the advantages of combining UVOT data with simultaneous and contemporaneous observations from the high-energy detectors on the Swift spacecraft. Multiple filters covering 1,800-6,000 Angstroms reveal a red source with spectral slope steeper than the simultaneous X-ray continuum. Spectral fits indicate that the UVOT colors are consistent with dust extinction by systems at z = 1.2037 and z = 1.4436, redshifts where absorption systems have been pre-identified. However, the data can be most-easily reproduced with models containing a foreground system of neutral gas redshifted by z = 2.8 +/- 0.3. For both of the above scenarios, spectral and decay slopes are, for the most part, consistent with fireball expansion into a uniform medium, provided a cooling break occurs between the energy ranges of the UVOT and Swifts X-ray instrumentation.Comment: 15 pages, 4 figures, ApJ Letters, in pres