12 research outputs found
Quantification de la biomasse d’Alternaria dauci dans des génotypes de carotte par PCR en temps réel
Alternaria dauci, agent causal des brûlures foliaires est pathogène sur différentes espèces végétales
Is quantitative resistance qualitative? An example with two Alternaria Leaf Blight resistant carrot genotypes and four resistance assessment techniques
International audienc
Evaluating aggressiveness and host range of Alternaria dauci in a controlled environment
The aggressiveness of Alternaria dauci isolates was investigated in greenhouse conditions. Twenty-seven isolates were pre-selected from a large collection to represent high diversity according to geographic or host origins and intergenic spacer (IGS) polymorphism. IGS sequence analysis revealed that isolates were grouped within three different clusters. Eleven isolates were selected and inoculated on a susceptible carrot cultivar. Three criteria (mean lesion number, mean necrotic leaf area and mean disease index) were used to assess the aggressiveness of isolates. Continuous variation in aggressiveness was shown and no clear division into isolate classes was evident. For the host range study, two isolates were inoculated under greenhouse conditions onto nine cultivated Apiaceae species, two wild Daucus species and six cultivated non-Apiaceae species representing six botanical families. Lesions varying in severity were observed on all dicot species (Apiaceae and non-Apiaceae), but no symptoms developed on the two monocots studied (leek and sweetcorn). Plant species were also differentiated on the basis of expanding lesions (cultivated and wild carrot, dill and fennel) or non-expanding lesions (other dicot species). Typical A. dauci conidia were observed after in vitro incubation of leaves with symptoms. Fungal structures were isolated from lesions and A. dauci was confirmed on the basis of conidial morphology and specific conventional PCR results. Genotyping of individual isolates performed with microsatellite markers confirmed the presence of the inoculated isolate. The results clearly showed that, in controlled conditions, the host range of A. dauci is not restricted to carrot
Evaluation of different methods for the characterization of carrot resistance to the alternaria leaf blight pathogen (Alternaria dauci) revealed two qualitatively different resistances
Alternaria leaf blight (ALB), caused by Alternaria dauci, is one of the most damaging foliar diseases of carrot worldwide. The aim of this study was to compare different methods for evaluating levels of carrot resistance to ALB. Three techniques were investigated by comparison with a visual disease assessment control: in vivo conidial germination, a bioassay based on a drop-inoculation method, and in planta quantification of fungal biomass by quantitative PCR (Q-PCR). Three carrot cultivars showing different degrees of resistance to A. dauci were used, i.e. a susceptible cultivar (Presto) and two partially resistant genotypes (Texto and Bolero), challenged with an aggressive or a very aggressive isolate of A. dauci. Both partially resistant genotypes produced a higher mean number of germ tubes per conidium (up to 3·42±0·35) than the susceptible one (1·26±0·18). The drop-inoculation results allowed one of the partially resistant genotypes (Bolero, log10(S+1) = 1·34±0·13) to be distinguished from the susceptible one (1·90±0·13). By contrast, fungal growth measured by Q-PCR clearly differentiated the two partially resistant genotypes with log10(I) values of 2·77±0·13 compared to the susceptible cultivar (3·65±0·13) at 15 days post-inoculation. This result was strongly correlated (r2 = 0·91) with the disease severity index scored at the same date. Data obtained with the different assessment methods strongly suggest that the Texto and Bolero genotypes have different genetic resistance sources
Evaluation dans différentes conditions environnementales de la résistance des variétés de carotte à Alternaria dauci: prise en compte du stade de développement de la plante et de l’agressivité de la souche
La phytoalexine '6-méthoxymélleine' est-t-elle impliquée dans les mécanismes de résistance de la carotte à Alternaria dauci, agent de la brûlure foliaire ?
Effets inhibiteurs de la phytoalexine 6-méthoxymelléine sur le développement d’Alternaria dauci, agent de la brûlure foliaire de la carotte.
Is the host range of Alternaria dauci restricted to carrot?
The host range of Alternaria dauci was investigated in a greenhouse. Two strains were inoculated onto nine cultivated Apiaceae species, two wild Daucus species and six cultivated non-Apiaceae species representing six botanic families. Lesions of various intensities were observed on all dicot species, but no symptoms developed on the two monocots studied. Typical A. dauci conidia were observed after in vitro incubation of necrotic leaves. Fungal strains were isolated from lesions and A. dauci was diagnosed on the basis of conidium morphology and specific conventional PCR findings. Genotyping of individual isolates performed with microsatellite markers confirmed the presence of the inoculated strain. Our results strongly suggest that species other than carrot can be alternative hosts. Those results are important to consider for a better management of Alternaria leaf blight in carrot fields