On the surface tension of fluctuating quasi-spherical vesicles

We calculate the stress tensor for a quasi-spherical vesicle and we thermally average it in order to obtain the actual, mechanical, surface tension $\tau$ of the vesicle. Both closed and poked vesicles are considered. We recover our results for $\tau$ by differentiating the free-energy with respect to the proper projected area. We show that $\tau$ may become negative well before the transition to oblate shapes and that it may reach quite large negative values in the case of small vesicles. This implies that spherical vesicles may have an inner pressure lower than the outer one.Comment: To appear in Eur. Phys. J. E, revised versio

Are stress-free membranes really 'tensionless'?

In recent years it has been argued that the tension parameter driving the fluctuations of fluid membranes, differs from the imposed lateral stress, the 'frame tension'. In particular, stress-free membranes were predicted to have a residual fluctuation tension. In the present paper, this argument is reconsidered and shown to be inherently inconsistent -- in the sense that a linearized theory, the Monge model, is used to predict a nonlinear effect. Furthermore, numerical simulations of one-dimensional stiff membranes are presented which clearly demonstrate, first, that the internal 'intrinsic' stress in membranes indeed differs from the frame tension as conjectured, but second, that the fluctuations are nevertheless driven by the frame tension. With this assumption, the predictions of the Monge model agree excellently with the simulation data for stiffness and tension values spanning several orders of magnitude

Reply to Comment on: "Are stress-free membranes really 'tensionless'?"

This is a reply to a comment on the paper arXiv:1204.2075 "Are stress-free membranes really tensionless ?" (EPL 95,28008 (2011))

Highly permeable macroporous polymers synthesized from pickering medium and high internal phase emulsion templates

Open porous poly-Plckerlng-M/HIPEs with permeabilities of up to 2.6 D were prepared by polymerisation of PickeringM/HIPEs to which small amounts of surfactant were added. The permeability of these poly-Pickering-M/HIPEs is more than 5 times that of conventional polyHI PEs. This approach allows the synthesis of a novel class of permeable particle reinforced macroporous polymers with significant potential for practical exploitation. (Figure Presented) © 2010 WILEY-VCH VerlagGmbH S.Co. KCaA, Weinheim

The effects of upcoding, cream skimming and readmissions on the Italian hospitals efficiency: a population–based investigation

In this paper we analyze the effects of some distortions induced by prospective payment system, i.e. Upcoding, Cream Skimming and Readmissions, on hospitals’ technical efficiency. We estimate a production function using a population–based dataset composed by all active hospitals in an Italian region during the period 1998–2007. We show that cream skimming and upcoding have a negative impact on hospitals’ technical efficiency, while readmissions have a positive effect. Moreover, we find that private hospitals are more engaged in cream skimming than public and not–for–profit ones, while we observe no ownership differences regarding upcoding. Not–for–profit hospitals have the highest readmission index. Last, not–for–profit and public hospitals have the same efficiency levels, while private hospitals have the lowest technical efficiency.Upcoding, Cream Skimming, Readmission, Hospital Technical Efficiency, Ownership.

Engineering muscle networks in 3D gelatin methacryloyl hydrogels: influence of mechanical stiffness and geometrical confinement

In this work, the influence of mechanical stiffness and geometrical confinement on the 3D culture of myoblast-laden gelatin methacryloyl (GelMA) photo-crosslinkable hydrogels was evaluated in terms of in vitro myogenesis. We formulated a set of cell-laden GelMA hydrogels with a compressive modulus in the range 1÷17 kPa, obtained by varying GelMA concentration and degree of cross-linking. C2C12 myoblasts were chosen as the cell model, to investigate the supportiveness of different GelMA hydrogels on myotube formation up to 2 weeks. Results showed that the hydrogels with a stiffness in the range 1÷3 kPa provided enhanced support to C2C12 differentiation in terms of myotube number, rate of formation and space distribution. Finally, we studied the influence of geometrical confinement on myotube orientation by confining cells within thin hydrogel slabs having different cross-sections: i) 2000×2000 m, ii) 1000×1000 m and iii) 500×500 m. The obtained results showed that by reducing the cross-section—i.e., by increasing the level of confinement—myotubes were more likely restrained and formed aligned myostructures that better mimicked the native morphology of skeletal muscle

Transforming Teaching in Inclusive Settings: An Educator Looks at VIM

Inclusive classrooms are those in which students with special needs are educated alongside their non-disabled peers. This article examines the attitudes that teachers in inclusive settings may hold that are believed to be barriers to successful inclusion. Given that historically, students with special needs in inclusive classrooms have made limited academic progress through traditional whole-class instruction, it is suggested that a transformation in inclusive teaching take place. This article suggests that educators apply Dallas Willard’s (2002) concept of VIM, outlined in his book, Renovation of the Heart. Christian teachers may find the vision, intention, and means Willard advocates to be useful in bringing about the desired transformation

Co-axial wet-spinning in 3D Bioprinting: state of the art and future perspective of microfluidic integration

Nowadays, 3D bioprinting technologies are rapidly emerging in the field of tissue engineering and regenerative medicine as effective tools enabling the fabrication of advanced tissue constructs that can recapitulate in vitro organ/tissue functions. Selecting the best strategy for bioink deposition is often challenging and time consuming process, as bioink properties-in the first instance, rheological and gelation-strongly influence the suitable paradigms for its deposition. In this short review, we critically discuss one of the available approaches used for bioprinting-namely co-axial wet-spinning extrusion. Such a deposition system, in fact, demonstrated to be promising in terms of printing resolution, shape fidelity and versatility when compared to other methods. An overview of the performances of co-axial technology in the deposition of cellularized hydrogel fibres is discussed, highlighting its main features. Furthermore, we show how this approach allows (i) to decouple the printing accuracy from bioink rheological behaviour-thus notably simplifying the development of new bioinks- A nd (ii) to build heterogeneous multi-materials and/or multicellular constructs that can better mimic the native tissues when combined with microfluidic systems. Finally, the ongoing challenges and the future perspectives for the ultimate fabrication of functional constructs for advanced research studies are highlighted. © 2018 IOP Publishing Ltd

Relationship between ethics and Fair Trade supply chain organisation and performance: the case of Italian Alternative Trade Organisations (ATOs)

Fair Trade related import from developing countries is quickly growing although it still represents a small share of the total import. Its influence on the development of rural areas in developing countries is related to both quantitative growth and the respect of its ethical code. Large food multi-national companies are increasingly interested in Fair Trade; part of the Fair Trade movement considers the risk of a related loss in the products identity; others consider the refusal of a more "professional" approach to Fair Trade management as a constraint to its growth. This debate is particularly felt in Italy. The goal of this paper is to evaluate how the most important Italian Fair Trade importers (ATOs) business models influenced their growth strategies; transaction costs analyisis and logistics performance indicators were adopted to measure the supply chain coordination efficiency and performance. The results showed that the ATOs growth strategies, and logistics performances, seemed more influenced by their value propositions, than the lack of managerial skill.Fair Trade, Logistics, Alternative Trade Organisations, Agribusiness, International Relations/Trade,