Uji Banding Efektivitas Ekstrak Buah Pare Belut(Trichosantes Anguina Linn) Dengan Zinc Pyrithione 1% Terhadap Pertumbuhan Pityrosporum Ovale Pada Penderita Berketombe

Background. Dandruff is a thin skuama hiperproliferation in scalp within itchysensation with or without inflammation. Snake gourd is one of natural ingredient thathave antifungal activity. Zinc pythitione very effective for inhibiting growth ofPityrosporum ovale that cause dandruff.Objective. To compare effectiveness of snake gourd extract (Trichosanthes AnguinaLinn.)100% with zinc pyrithione 1% in inhibiting the growth of Pityrosporum ovalein patients with dandruff in vitro.Method. This was an experimental laboratories study with Post test only controlgroup design. Subject was 30 patients with dandruff. Examination materials wasscraping scalp of patient that suffers dandruff. Examined microscopically in 10%KOH plus parker blue black ink. Positive culture results were diluted in sterile 0.9%NaCl solution and made the same turbidity with a solution of 0.5 McFarlan 0.1 cc isthen taken and implanted in the SDA media containing olive oil and snake gourdextract 100% and zinc pyrithione 1%. Data were analyzed using chi square test orFischer-exact test with significance p value <0.05.Result. From 30 SDA media containing olive oil extract of snake groud 100%, 25(41.7%) had P.ovale negative (-) and 5 (8.3%) tubes have a positive P.ovale (+).From 30 media SDA olive oil containing zinc pyrithione 1%, 1 (1.7%%) hadPityrosporum ovale positive (+) and 29 (48.3%) had P. ovale negative (-). Terms ofchi-square test is not fulfilled, because there were two cells that have less expectationof 5 (1 and 5), so the test method used by the Fischer-exact p = 0.195, which meansthere is no significant difference between the effects of pare belut fruit extract withzinc pythirione 1%.Conclusion. Effectiveness of Snake groud ( T.Anguina Linn.) extract 100% equalwith zinc pyrithione 1% to inhibited the growth of P. ovale.Key word. Dandruff, P. ovale, pare belut extract 100%, zinc pythirione 1%

The MDA and HSP70 Concentration in Preeclamptic Patient Placenta\u27s

Preeclampsia is a disease in pregnancy and characterized by hypertension and proteinuria. Preeclampsia and eclampsia are the most causes of maternal and fetal mortality and morbidity in Indonesia. Placental and systemic oxidative stress caused endothelial cell dysfunction and injury. Placental oxidative stress also linked to fetal growth restriction. HSP70 is essential for cellular recovery, survival and maintenance of homeostasis. The purpose of this study was to compare the MDA, a marker for oxidative stress and HSP70 production in placental of severe preeclampsia, mild preeclampsia and normotensive pregnant women. Placenta were collected after delivery from normotensive pregnancies (N=10), severe preeclampsia (N=10) and mild preeclampsia (N=10). Placenta was cultured in RPMI and 20% FBS, and supernatant were collected in day 3. MDA was measured using spectrophotometer and absorbance read in 530nm. HSP70 was measured using enzyme-linked immunosorbent assay. The mean MDA concentration did not differ significantly between patients with severe preeclampsia (7.13+5.36 nmol/ml) and mild preeclampsia (4.82+2.47 nmol/ml) when compared with normotensive pregnancies (4.57+2.4 nmol/ml). The mean HSP70 concentration in mild preeclampsia is highest (10.15+12.39 nmo/ml) when compared with severe preeclampsia (3.78 +3.07 nmol/ml) and normotensive pregnant women (3.76+4.65nmol/ml), but the difference was not significant. Although the difference was not significant, is indicates homeostasis response in mild preclampsia women is relative good. This response was abated in severe preeclampstic women. Although MDA and HSP70 concentration did not differ significantly between groups, however the high HSP70 concentration is indicates homeostasis response relatively good in mild preeclamptic women

Endothelial cell culture from human umbilical cord vein

[no abstract available

Does VEGF concentration in pre-eclamptic serum induce sVCAM-1 production in endothelial cell culture?

<p>Serum concentrations of VEGF (Vascular Endothelial Growth Factor) are elevated in preeclampsia. In addition to inducing mitosis and increase permeability of endothelial cells, VEGF was reported to activate endothelial cells to produce cell adhesion molecules. Cell adhesion molecules play an important role in the inflammation process by inducing adherence of leukocytes in blood stream to the endothelial cells. The aim of this study is to investigate the effect of VEGF in serum from preeclamptic patients on sVCAM-1 (soluble vascular adhesion molecules-1) production in endothelial cell culture. Twelve sera from women with preeclampsia and 11 from women with normal pregnancy (controls) in 20% concentration were added to human umbilical vein endothelial cell culture (HUVEC) and incubated for 24 hours. All subjects have agreed to participate in this study and signed the informed consent form. sVCAM-1 concentration in the supernatant was measured by ELISA. VEGF concentration tends to be higher in preeclamptic serum than control, but the difference is not stastitically significant. The production of sVCAM-1 by endothelial cells exposed to preeclamptic serum was significantly higher than the production by endothelial cells exposed to serum from control (p&lt;0.05). No correlation was found between the difference in VEGF concentrations in preeclamptic and control sera, and sVCAM-1 production by endothelial cell culture. <em><strong><em>(Med J Indones 2005; 14: 3-6)</em></strong></em></p><p><strong>Keywords:</strong> <em>endothelial cell, preeclampsia, VCAM, VEGF</em></p