24 research outputs found
Detection of chitinase activity and its characterization from Pseudomonas fluorescens of tea rhizosphere
Please see the pdf file here: http://indsocplantationcrops.in/journal.ph
Bio-efficacy of pumpkin phloem lectin on red spider mite, Oligonychus coffeae (Nietner) infesting tea
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Auxin pretreatment promotes regeneration of sugarcane (Saccharum spp. hybrids) midrib segment explants
We have developed a new, simple,
quick and genotype-independent method for direct
regeneration of sugarcane using novel midrib
segment explants. Our protocol involves two
steps: the pretreatment of starting material on MS
(Murashige and Skoog (1962) Physiol Plant
15:473–497) medium containing 3.0 mg/l 2,4-
dichlorophenoxyacetic acid (2,4-D) for 8 days
under continuous dark and subsequent transfer of
the explants to MS medium augmented with
0.1 mg/l benzyladenine (BA) and 0.1 mg/l naphthaleneacetic
acid (NAA) under light-dark conditions.
On the regeneration medium, numerous
globular structures appeared from the explants
and subsequently differentiated into shoots.
Regenerated shoots attained 2–5 cm height
within 30 days of culture initiation and readily
rooted on MS basal medium. Hardened plants
were successfully established in the greenhouse.
The regulation of sugarcane morphogenesis by
auxin pretreatment is discussed
Molecular characterization of bacterial biocontrol agents and their chitinase genes from tea soil
395-401In tea, bacterial biocontrol agents viz. Bacillus and Pseudomonas and an enzyme like chitinase from these bacterial strains are used to control tea pests and pathogens. However, literature on molecular identification of the same is quite scarce. In this study, Bacillus and Pseudomonas strains isolated from tea soil samples, were systematically identified by 16S rRNA sequencing. Molecular characterization of bacteria was carried out to identify the species of different level chitinase producing bacteria and diversity among them. Further, chitinase gene was characterized from these bacteria to understand the gene diversity among different bacterial chitinase that has potential application in controlling the plant pests and pathogens. Sequence analysis of 16S rRNA and chitinase gene sequences was made among thirteen Bacillus and five Pseudomonas species submitted in NCBI Genbank
Orthodontic-periodontic interdisciplinary approach: A literature review
In the last few years, there has been a major advancement in the orthodontic treatment of adult patients. Adult orthodontics differs from children's orthodontics in that there is virtually no more growth in adults and that the tooth-supporting structures, the periodontium, have changed.Because orthodontic treatment is done through the periodontium, having a healthy tooth supporting system is a must. At the same time, when the tooth moves under the influence of orthodontic treatment, the periodontium undergoes a variety of modifications. This review article discusses relationship between periodontal tissue and orthodontic treatment
Characterization of ginger rhizome lectin and its efficacy in controlling red spider mite in tea
Methodology was developed to isolate and characterize the ginger rhizome lectin and its applied aspects in integrated pest management in tea. Lectin, a defense protein with mannose specificity was purified from fresh rhizomes of ginger (Zingiber officinale Roscoe) by affinity chromatography. Purified fractions of lectin showed agglutination with rabbit erythrocytes. SDS PAGE analysis revealed the presence of 15 KDa ginger rhizome lectin. Bio-efficacy of purified ginger rhizome lectin was experimented on red spider mite (Oligonychus coffeae Nietner) under in-vitro conditions. It was observed that 100 µg ml–1 of purified lectin caused in toto mortality of adult red spider mite. Identical results were observed in terms of ovipositional deterrence of red spider mite at 100 µg ml–1 of ginger rhizome lectin. Results suggest that ginger rhizome could be used as an alternative plant source for lectin isolation and it can be used as an acaricide in the IPM schedule or organic tea fields
Optimization of the Impeller Design for Mesenchymal Stem Cell Culture on Microcarriers in Bioreactors
When agitating mesenchymal stem cells adhered on microcarriers in bioreactors, a compromise has to be found between sufficient particle suspension and limitation of hydromechanical stresses. The present study proposes a strategy to improve the design of an ‘elephant ear' impeller at the just-suspended state by varying its relative size, blade slope angle, and position in the reactor. To do that, computational fluid dynamics simulations were coupled with multi-objective optimization to minimize the hydromechanical stress encountered by the microcarriers. Two minimization criteria were considered: (P/V)@p and the energy dissipation function EDC. On the basis of 31 conditions, an optimal impeller geometry is proposed. © 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinhei