378,069 research outputs found
Studying Membrane Biogenesis with a Luciferase-Based Reporter Gene Assay
To study the coordination of different lipid synthesis pathways during membrane biogenesis it is useful to work with an experimental system where membrane biogenesis occurs rapidly and in an inducible manner. We have found that phagocytosis of latex beads is practical for these purposes as cells rapidly synthesize membrane lipids to replenish membrane pools lost as wrapping material during particle engulfment. Here, we describe procedures for studying changes in phagocytosis-induced gene expression with a luciferase-based reporter gene approach using the Dual-Glo Luciferase Assay System from Promega
An optimal synchronous bandwidth allocation scheme for guaranteeing synchronous message deadlines with the timed-token MAC protocol
This paper investigates the inherent timing properties of the timed-token medium access control (MAC) protocol necessary to guarantee synchronous message deadlines in a timed token ring network such as, fiber distributed data interface (FDDI), where the timed-token MAC protocol is employed. As a result, an exact upper bound, tighter than previously published, on the elapse time between any number of successive token arrivals at a particular node has been derived. Based on the exact protocol timing property, an optimal synchronous bandwidth allocation (SBA) scheme named enhanced MCA (EMCA) for guaranteeing synchronous messages with deadlines equal to periods in length is proposed. Thm scheme is an enhancement on the previously publiibed MCA scheme
Holonomic Quantum Computing Based on the Stark Effect
We propose a spin manipulation technique based entirely on electric fields
applied to acceptor states in -type semiconductors with spin-orbit coupling.
While interesting in its own right, the technique can also be used to implement
fault-resilient holonomic quantum computing. We explicitly compute adiabatic
transformation matrix (holonomy) of the degenerate states and comment on the
feasibility of the scheme as an experimental technique.Comment: 5 page
Cycle-time properties of the timed token medium access control protocol
We investigate the timing properties of the timed token protocol that are necessary to guarantee synchronous message deadlines. A tighter upper bound on the elapse time between the token's lth arrival at any node i and its (l + v)th arrival at any node k is found. A formal proof to this generalized bound is presented
Auxiliary-field quantum Monte Carlo study of first- and second-row post-d elements
A series of calculations for the first- and second-row post-d elements (Ga-Br
and In-I) are presented using the phaseless auxiliary-field quantum Monte Carlo
(AF QMC) method. This method is formulated in a Hilbert space defined by any
chosen one-particle basis, and maps the many-body problem into a linear
combination of independent-particle solutions with external auxiliary fields.
The phase/sign problem is handled approximately by the phaseless formalism
using a trial wave function, which in our calculations was chosen to be the
Hartree-Fock solution. We used the consistent correlated basis sets of Peterson
and coworkers, which employ a small core relativistic pseudopotential. The AF
QMC results are compared with experiment and with those from density-functional
(GGA and B3LYP) and coupled-cluster CCSD(T) calculations. The AF QMC total
energies agree with CCSD(T) to within a few milli-hartrees across the systems
and over several basis sets. The calculated atomic electron affinities,
ionization energies, and spectroscopic properties of dimers are, at large basis
sets, in excellent agreement with experiment.Comment: 10 pages, 2 figures. To be published in Journal of Chemical Physic
Auxiliary-field quantum Monte Carlo calculations of molecular systems with a Gaussian basis
We extend the recently introduced phaseless auxiliary-field quantum Monte
Carlo (QMC) approach to any single-particle basis, and apply it to molecular
systems with Gaussian basis sets. QMC methods in general scale favorably with
system size, as a low power. A QMC approach with auxiliary fields in principle
allows an exact solution of the Schrodinger equation in the chosen basis.
However, the well-known sign/phase problem causes the statistical noise to
increase exponentially. The phaseless method controls this problem by
constraining the paths in the auxiliary-field path integrals with an
approximate phase condition that depends on a trial wave function. In the
present calculations, the trial wave function is a single Slater determinant
from a Hartree-Fock calculation. The calculated all-electron total energies
show typical systematic errors of no more than a few milli-Hartrees compared to
exact results. At equilibrium geometries in the molecules we studied, this
accuracy is roughly comparable to that of coupled-cluster with single and
double excitations and with non-iterative triples, CCSD(T). For stretched bonds
in HO, our method exhibits better overall accuracy and a more uniform
behavior than CCSD(T).Comment: 11 pages, 5 figures. submitted to JC
The Central Engines of Gamma-Ray Bursts
Leading models for the "central engine" of long, soft gamma-ray bursts (GRBs)
are briefly reviewed with emphasis on the collapsar model. Growing evidence
supports the hypothesis that GRBs are a supernova-like phenomenon occurring in
star forming regions, differing from ordinary supernovae in that a large
fraction of their energy is concentrated in highly relativistic jets. The
possible progenitors and physics of such explosions are discussed and the
important role of the interaction of the emerging relativistic jet with the
collapsing star is emphasized. This interaction may be responsible for most of
the time structure seen in long, soft GRBs. What we have called "GRBs" may
actually be a diverse set of phenomena with a key parameter being the angle at
which the burst is observed. GRB 980425/SN 1988bw and the recently discovered
hard x-ray flashes may be examples of this diversity.Comment: 8 pages, Proc. Woods Hole GRB meeting, Nov 5 - 9 WoodsHole
Massachusetts, Ed. Roland Vanderspe
Fallback and Black Hole Production in Massive Stars
The compact remnants of core collapse supernovae - neutron stars and black
holes - have properties that reflect both the structure of their stellar
progenitors and the physics of the explosion. In particular, the masses of
these remnants are sensitive to the density structure of the presupernova star
and to the explosion energy. To a considerable extent, the final mass is
determined by the ``fallback'', during the explosion, of matter that initially
moves outwards, yet ultimately fails to escape. We consider here the simulated
explosion of a large number of massive stars (10 to 100 \Msun) of Population I
(solar metallicity) and III (zero metallicity), and find systematic differences
in the remnant mass distributions. As pointed out by Chevalier(1989),
supernovae in more compact progenitor stars have stronger reverse shocks and
experience more fallback. For Population III stars above about 25 \Msun and
explosion energies less than erg, black holes are a common
outcome, with masses that increase monotonically with increasing main sequence
mass up to a maximum hole mass of about 35 \Msun. If such stars produce primary
nitrogen, however, their black holes are systematically smaller. For modern
supernovae with nearly solar metallicity, black hole production is much less
frequent and the typical masses, which depend sensitively on explosion energy,
are smaller. We explore the neutron star initial mass function for both
populations and, for reasonable assumptions about the initial mass cut of the
explosion, find good agreement with the average of observed masses of neutron
stars in binaries. We also find evidence for a bimodal distribution of neutron
star masses with a spike around 1.2 \Msun (gravitational mass) and a broader
distribution peaked around 1.4 \Msun.Comment: Accepted for publication in Ap
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