Psoralen-Deoxyribonucleic Acid Photoreaction. Characterization of the Monoaddition Products from 8-Methoxypsoralen and 4,5', 8-Trimethylpsoralent

ABSTRACT: The isolation and structural characterization are described of the major monoaddition products formed in the photoreaction of two naturally occurring psoralens, 8-methoxypsoralen and 4,5',8-trimethylpsoralen, with high molecular weight, double-stranded DNA. Hydrolysis of the psoralenmodified DNA and subsequent chromatography resulted in the isolation of four modified nucleosides from each psoralen. Structural characterization was accomplished by mass spectrometry and 'H N M R analysis. The major products, accounting for 44-52% of the covalently bound psoralen, are two diastereomeric thymidine adducts formed by cycloaddition between the 5,6 double bond of the pyrimidine and the 4',5' (furan) double bond of the psoralen. A minor product, less than 2% of the covalently bound psoralen, is a furan-side E o r a l e n s or furocoumarins are a class of compounds found in a wide variety of plants and'fungi and have been used since ancient times as dermal photosensitizing agents for the treatment of various skin pigmentation disorder

Electrophysiological correlates of cross-linguistic semantic integration in hearing signers:. N400 and LPC

We explored semantic integration mechanisms in native and non-native hearing users of sign language and non-signing controls. Event-related brain potentials (ERPs) were recorded while participants performed a semantic decision task for priming lexeme pairs. Pairs were presented either within speech or across speech and sign language. Target-related ERP responses were subjected to principal component analyses (PCA), and neurocognitive basis of semantic integration processes were assessed by analyzing the N400 and the late positive complex (LPC) components in response to spoken (auditory) and signed (visual) antonymic and unrelated targets. Semantically-related effects triggered across modalities would indicate a similar tight interconnection between the signers&#39; two languages like that described for spoken language bilinguals. Remarkable structural similarity of the N400 and LPC components with varying group differences between the spoken and signed targets were found. The LPC was the dominant response. The controls&#39; LPC differed from the LPC of the two signing groups. It was reduced to the auditory unrelated targets and was less frontal for all the visual targets. The visual LPC was more broadly distributed in native than non-native signers and was left-lateralized for the unrelated targets in the native hearing signers only. Semantic priming effects were found for the auditory N400 in all groups, but only native hearing signers revealed a clear N400 effect to the visual targets. Surprisingly, the non-native signers revealed no semantically-related processing effect to the visual targets reflected in the N400 or the LPC; instead they appeared to rely more on visual post-lexical analyzing stages than native signers. We conclude that native and non-native signers employed different processing strategies to integrate signed and spoken semantic content. It appeared that the signers&#39; semantic processing system was affected by group-specific factors like language background and/or usage.</p

Selected 'Starter kit' energy system modelling data for selected countries in Africa, East Asia, and South America (#CCG, 2021)

Energy system modeling can be used to develop internally-consistent quantified scenarios. These provide key insights needed to mobilise finance, understand market development, infrastructure deployment and the associated role of institutions, and generally support improved policymaking. However, access to data is often a barrier to starting energy system modeling, especially in developing countries, thereby causing delays to decision making. Therefore, this article provides data that can be used to create a simple zero-order energy system model for a range of developing countries in Africa, East Asia, and South America, which can act as a starting point for further model development and scenario analysis. The data are collected entirely from publicly available and accessible sources, including the websites and databases of international organisations, journal articles, and existing modeling studies. This means that the datasets can be easily updated based on the latest available information or more detailed and accurate local data. As an example, these data were also used to calibrate a simple energy system model for Kenya using the Open Source Energy Modeling System (OSeMOSYS) and three stylized scenarios (Fossil Future, Least Cost and Net Zero by 2050) for 2020–2050. The assumptions used and the results of these scenarios are presented in the appendix as an illustrative example of what can be done with these data. This simple model can be adapted and further developed by in-country analysts and academics, providing a platform for future work

Orthorhombic distortion on Li intercalation in anatase

Published versio

Processing of Abstract Rule Violations in Audition

The ability to encode rules and to detect rule-violating events outside the focus of attention is vital for adaptive behavior. Our brain recordings reveal that violations of abstract auditory rules are processed even when the sounds are unattended. When subjects performed a task related to the sounds but not to the rule, rule violations impaired task performance and activated a network involving supratemporal, parietal and frontal areas although none of the subjects acquired explicit knowledge of the rule or became aware of rule violations. When subjects tried to behaviorally detect rule violations, the brain's automatic violation detection facilitated intentional detection. This shows the brain's capacity for abstraction – an important cognitive function necessary to model the world. Our study provides the first evidence for the task-independence (i.e. automaticity) of this ability to encode abstract rules and for its immediate consequences for subsequent mental processes

Signs in the brain: Hearing signers’ cross-linguistic semantic integration strategies

Abstract Audio-oral speech and visuo-manual sign language as used by the Deaf community are two very different realizations of the human linguistic communication system. Sign language is not only used by the hearing impaired but also by different groups of hearing individuals. To date, there is a great discrepancy in scientific knowledge about signed and spoken languages. Particularly little is known about the integration of the two systems, even though the vast majority of deaf and hearing signers also have a command of some form of speech. This neurolinguistic study aimed to achieve basic knowledge about semantic integration mechanisms across speech and sign language in hearing native and non-native signers. Basic principles of sign processing as reflected in electrocortical brain activation and behavioral decisions were examined in three groups of study participants: Hearing native signers (children of deaf adults, CODAs), hearing late learned signers (professional sign language interpreters), and hearing non-signing controls. Event-related brain potentials (ERPs) and behavioral response frequencies were recorded while the participants performed a semantic decision task for priming lexeme pairs. The lexeme pairs were presented either within speech (spoken prime-spoken target) or across speech and sign language (spoken prime-signed target). Target-related ERP responses were subjected to temporal principal component analyses (tPCA). The neurocognitive basis of semantic integration processes were assessed by analyzing different ERP components (N170, N400, late positive complex) in response to the antonymic and unrelated targets. Behavioral decision sensitivity to the target lexemes is discussed in relation to the measured brain activity. Behaviorally, all three groups of study participants performed above chance level when making semantic decisions about the primed targets. Different result patterns, however, hinted at three different processing strategies. As the target-locked electrophysiological data was analyzed by PCA, for the first time in the context of sign language processing, objectively allocated ERP components of interest could be explored. A little surprisingly, the overall study results from the sign-naïve control group showed that they performed in a more content-guided way than expected. This suggested that even non-experts in the field of sign language were equipped with basic skills to process the cross-linguistically primed signs. Behavioral and electrophysiological study results together further brought up qualitative differences in processing between the native and late learned signers, which raised the question: can a unitary model of sign processing do justice to different groups of sign language users?Tiivistelmä Kuuloaistiin ja ääntöelimistön motoriikkaan perustuva puhe ja kuurojen yhteisön käyttämä, näköaistiin ja käsien liikkeisiin perustuva viittomakieli ovat kaksi varsin erilaista ihmisen kielellisen viestintäjärjestelmän toteutumismuotoa. Viittomakieltä käyttävät kuulovammaisten ohella myös monet kuulevat ihmisryhmät. Tähänastinen tutkimustiedon määrä viittomakielistä ja puhutuista kielistä eroaa huomattavasti. Erityisen vähän on tiedetty näiden kahden järjestelmän yhdistämisestä, vaikka valtaosa kuuroista ja kuulevista viittomakielen käyttäjistä hallitsee myös puheen jossain muodossa. Tämän neurolingvistisen tutkimuksen tarkoituksena oli hankkia perustietoja puheen ja viittomakielen välisistä semanttisista yhdistämismekanismeista kuulevilla, viittomakieltä äidinkielenään tai muuna kielenä käyttävillä henkilöillä. Viittomien prosessoinnin perusperiaatteita, jotka ilmenevät aivojen sähköisen toiminnan muutoksina ja valintapäätöksinä, tutkittiin kolmessa koehenkilöryhmässä: kuulevilla viittomakieltä äidinkielenään käyttävillä henkilöillä (kuurojen aikuisten kuulevilla ns. CODA-lapsilla, engl. children of deaf adults), kuulevilla viittomakielen myöhemmin oppineilla henkilöillä (viittomakielen ammattitulkeilla) sekä kuulevilla viittomakieltä osaamattomilla verrokkihenkilöillä. Tapahtumasidonnaiset herätepotentiaalit (ERP:t) ja käyttäytymisvasteen frekvenssit rekisteröitiin koehenkilöiden tehdessä semanttisia valintoja viritetyistä (engl. primed) lekseemipareista. Lekseemiparit esitettiin joko puheena (puhuttu viritesana – puhuttu kohdesana) tai puheen ja viittomakielen välillä (puhuttu viritesana – viitottu kohdesana). Kohdesidonnaisille ERP-vasteille tehtiin temporaaliset pääkomponenttianalyysit (tPCA). Semanttisten yhdistämisprosessien neurokognitiivista perustaa arvioitiin analysoimalla erilaisia ERP-komponentteja (N170, N400, myöhäinen positiivinen kompleksi) vastineina antonyymisiin ja toisiinsa liittymättömiin kohteisiin. Käyttäytymispäätöksen herkkyyttä kohdelekseemeille tarkastellaan suhteessa mitattuun aivojen aktiviteettiin. Käyttäytymisen osalta kaikki kolme koehenkilöryhmää suoriutuivat satunnaistasoa paremmin tehdessään semanttisia valintoja viritetyistä kohdelekseemeistä. Erilaiset tulosmallit viittaavat kuitenkin kolmeen erilaiseen prosessointistrategiaan. Kun kohdelukittua elektrofysiologista dataa analysoitiin pääkomponenttianalyysin avulla ensimmäistä kertaa viittomakielen prosessoinnin yhteydessä, voitiin tutkia tarkkaavaisuuden objektiivisesti allokoituja ERP-komponentteja. Oli jossain määrin yllättävää, että viittomakielellisesti natiivin verrokkiryhmän tulokset osoittivat sen jäsenten toimivan odotettua sisältölähtöisemmin. Tämä viittaa siihen, että viittomakieleen perehtymättömilläkin henkilöillä on perustaidot lingvistisesti ristiin viritettyjen viittomien prosessointiin. Yhdessä käyttäytymisperäiset ja elektrofysiologiset tutkimustulokset toivat esiin laadullisia eroja prosessoinnissa viittomakieltä äidinkielenään puhuvien henkilöiden ja kielen myöhemmin oppineiden henkilöiden välillä. Tämä puolestaan johtaa kysymykseen, voiko yksi viittomien prosessointimalli soveltua erilaisille viittomakielen käyttäjäryhmille