Lignani biljne vrste Achillea lingulata

Five lignans with a 2.6-diaryl-3.7-dioabicyclo[3,3.0]octane skeleton epieudesmin. kobusin, pinoresinol. fargesin and sesartemin, were isolated from the aerial parts and roots, of Achillea lingulata. Their structures were identified by comparison of their H-1-NMR and MS data to those in the literature. Fargesin and pinoresinol have not been isolated previously from any species of the genus Achillea.Iz nadzemnog dela i korena biljne vrste Achillea lingulata izolovano je pet lignana 2,6-diaril-3.7-dioksabicikloŠ3.3.0Ćoktanskog tipa. To su epieudesmin, kobusin, pinorezinol, fargezin i sezartemin. Izolovani lignani su identifikovani na osnovu identičnosti wihovih NMR i masenih spektara sa spektrima iz literature. Fargezin i pinorezinol nisu bili do sada izolovani iz biljnih vrsta roda Achillea

Compositional characteristics of the essential oil of Pimpinella anisum and Foeniculum vulgare grown in Serbia

The essential oils obtained by hydrodistillation from the mature fruits of Pimpinella anisum and Foeniculum vulgare were analyzed by GC-MS. The oil of aniseed was characterized by higher amounts of trans-anethole (96.80%) than fennel essential oil (83.43%). The isomer of trans-anethole, methyl chavicol (estragol) was also present in both plants, with 1.36% in fennel and 0.19% in aniseed. Apart from these two phenylpropanoids, nine sesquiterpenes and one monoterpene were present in essential oil of Pimpinella anisum, which constituted the other 3.01% of essential oil, but only γ-himachalene was present with more than 1%. In the case of fennel, eight monoterpenes constituted the other 15.21% of essential oil, and limonene and fenchone were present with contributions of 9.34 and 4.58%, respectively. © 2015 Institute of Botany and Botanical Garden Jevremovac, Belgrade

Antifungalna aktivnost terpenoida davanonskog tipa izolovanih iz Artemisia lobelii Var. canescens

Five tetrahydrofuran sesquiterpenes, so-called davanones, and coumarin umbelliferone isolated from Artemisia lobelii All. var. canescens (DC.) Briqu, were tested for antifungal activity. All the compounds inhibited the growth of the applied fungi. The overall activity of one of them, 2-hydroxy-2,6,10-trimethyl-7,10-epoxy-dodeca-3,11-dien-5-on, was comparable to that of the antibiotic bifonazole.Pet tetrahidrofuranskih terpenoida davanonskog tipa i kumarin umbeliferon koji su prethodno izolovani iz biljne vrste Aretemisia lobelii var. conescens ispitivani su na antifungalnu aktivnost. Sva testirana jedinjenja inhibiraju rast primenjenih kultura gljiva. Najveću aktivnost, koja se može meriti sa aktivnošću komercijalnog antibiotika bifonazola, pokazuje 2-hidroksi-2,6,10-trimetil-7,10-epoksidodeka-3,11-dien-5-on

Flavonoidi iz cvetova Cephalaria pastricensis i njihova antiradikalska aktivnost

Two flavonoid glycosides 1 and 2 having the luteolin structure were isolated from flowers of the endemic plant species Cephalaria pastricensis. They were identified by (1)H and (13)C NMR, as well as UV/Vis spectroscopy. The structures of 1 and 2 were also confirmed by the spectral data of aglycones and TLC of the sugars obtained after acid hydrolysis. Flavones 1 and 2 showed significant antiradical activity in the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay.Iz cvetova Cephalaria pastricensis izolovana su dva heterozidna flavonoida sa luteolinskim aglikonom (1 i 2). Wihova struktura je određena primenom 1H i 13C-NMR i UV spektroskopije. Strukture flavona 1 i 2 su potvrđene i spektrima aglikona, odnosno tankoslojnom hromatografijom oslobođenih šećera nakon kisele hidrolize. Jedinjenja 1 i 2 su pokazala značajnu antiradikalsku aktivnost u 1,1-difenil-2 pikrilhidrazil (DPPH) testu

Fitohemijsko ispitivanje biljne vrste Anthemis cotula

The investigation of roots of Anthemis cotula (Asteraceae) from cast Serbia revealed, in addition to polyacetylenes previously isolated from the same species, three prenylated 4-hydroxyacetophenones, detected for the first time in any Anthemis species. It is possible that they act as phytoalexins in the plant. From the aerial parts, six linear sesquiterpene lactones (four known and two new ones), as well as two known flavones, apigenin and hispidulin, were isolated.Novo ispitivanje korena biljke A. cotula (Asteraceae) pokazalo je, pored četiri već izolovana poliacetilena, i prisustvo tri prenilovana 4-hidroksiacetofenona koji do sada nisu bili izolovani iz ovog roda. Iz nadzemnog dela biljke izolovano je šest linearnih seskviterpenskih laktona, od koji su dva nova, dok su preostali ranije nađeni u istoj biljci. Takođe su u nadzemnom delu identifikovana dva poznata flavona, apigenin i hispidulin

Antiproliferative Activity of Natural Flavonoid Fustin Isolated from the Heartwood of Cotinus Coggygria Scop. Against Breast and Colon Cancer Cell Lines

Background and objective: Cotinus coggygria Scop. is a valuable medicinal plant species with pronounced pharmacological potential due to its numerous biological activities. The herb is characterized by a high content of polyphenols among which is fustin. The anticancer activities of fustin, however, are extremely weakly studied. The aim of the present study was to investigate the in vitro antiproliferative potential of fustin isolated from the heartwood of C. coggygria against cell lines originating from two of the most common cancer types – breast (MDA-MB-231 and MCF7), and colon cancer (Colon 26). Materials and methods: Cell growth inhibitory properties of fustin were examined by MTT assay. Subsequently, phase-contrast and fl uorescence microscopy analysis as well as colonyforming assay were carried out on the most sensitive to the cytostatic action of the fustin cell line. Results: The obtained results showed that fustin reduced the proliferation of all studied cell lines. The highest cytostatic eff ect was registered towards breast cancer MDA-MB-231 cells with a half maximal inhibitory concentration (IC50) value of 56.02 μg/ ml followed by colon cancer cells with an IC50 of 78.07 μg/ml. MCF7 cell proliferation was least aff ected with a calculated IC50 of 187.8 μg/ml. Further investigations on breast cancer MDA-MB-231 cells indicated decreased density of cell monolayer and some morphological alterations, signifi cant attenuation in the number of viable cells, and diminished clonogenic ability of cells after fustin exposure. Conclusion: It could be concluded that fustin isolated from the heartwood of medicinal plant C. coggygria possesses marked antiproliferative properties against breast cancer cell line MDA-MB-231 which will be a subject of our more detailed future investigations

Inhibition potency of disulphides and trisulphides on various tumor cell lines growth

Tumor is one of the leading diseases of today. Chemotherapy and radiation therapy healing chances are limited, so some alternative methods are resorted to. Many papers indicated that garlic and organosulphur compounds diallyldisulphide (DADS) and diallyltrisulphide, which are main components of garlic decrease the cancer risk and inhibit the cell proliferation. In this paper inhibition potency of disulphides (DADS and its synthetic analogues) and trisulphides with different alkyl and phenyl substituents on growth of various tumor (Non-Small cell Lung Cancer, Colon cancer, CNS Cancer, Melanoma, Ovarian Cancer, Renal Cancer, Prostate Cancer, Breast Cancer, Leukemia) cell lines was investigated. Concentration of each compound (DADS, diethyl disulphide, dipropyl disulphide, diphenyl disulphide, tetraethylthiuram disulphide (TETUDS), dimetil trisulphide, dipropyl trisulphide and metilpropyl trisulphide (MPTS) was 10 μmol/L. It was found that DADS has an inhibitory effect on the growth of several cancer cell lines, but synthetic analogues TETUDS and MPTS exhibit stronger effect on certain cells lines. MPTS inhibits the growth of even 12 cell lines for more than 10%, especially leukemia cell line SR and NCI-H522 cell line of Non-Small cell Lung Cancer (reduction of growth for 24% and 47%, resp.). Trisulphide analogues exhibit little higher inhibitory effects in comparison to disulphide ones

Nonacosan-10-ol and n-Alkanes in Leaves of Pinus pinaster

In leaf cuticular wax of Pinus pinaster, content of nonacosan-10-ol is high (77.1% on average). n-Alkanes ranged from C18 to C35 with the most dominant C29 (24.8%). The carbon preference index (CPItotal) ranged from 3.1 to 5.6 (4.0 on average), while the average chain length (ACLtotal) ranged from 14.0 to 17.0 (14.8 on average). Long-chain n-alkanes (n-C25-35) strongly dominated (80.1%) over middle-chain (n-C21-24= 18.9%) and short-chain (n-C18-20= 0.9%) n-alkanes

Chemical Composition, Total Phenols and Flavonoids Contents and Antioxidant Activity as Nutritive Potential of Roasted Hazelnut Skins (Corylus avellana L.)

The present study evaluates natural composition of Serbian roasted hazelnut skins (HS) with potential role in application as functional nutrient of various food products. Total phenols (TPC) and flavonoids contents (TFC) in HS extracts obtained with dierent ethanol concentrations (10%—I, 50%—II and 96%—III) and their antioxidant activities were investigated. The highest total phenols content (706.0 9.7 mgGAE/gextract) was observed in 96% ethanol HS extract. Ethanol HS extracts showed very high antioxidant activity with eective concentrations (EC50) ranged between 0.052 and 0.066 mg/mL. The phenol and flavonoid content of roasted HS extracts I–III was determined by HPLC-ESI-MS/MS analyses. Contents of lipids, proteins, carbohydrates, metals, and C, H, N, S elements in roasted HS were also determined. Relatively high C/N, C/P and C/N/P ratios, rich metal contents and fatty acids composition indicated that hazelnut skin might be a good candidate for use as either human or fungal functional nutrient. In addition, possible application of phenolic HS extracts as UV booster was studied by recording UV spectra (220–440 nm) of 10 mg/L of HS extracts I–III combined with 10 mg/L of chemical sunscreen agent benzophenone-3 and in vitro sun protection factor (SPF) was calculated

Supplementary data for article: Novaković, M. M.; Stanković, M.; Vučković, I. M.; Todorović, N.; Trifunović, S. S.; Tešević, V.; Vajs, V.; Milosavljević, S. M. Diarylheptanoids from Alnus Glutinosa Bark and Their Chemoprotective Effect on Human Lymphocytes DNA. Planta Medica 2013, 79 (6), 499–505. https://doi.org/10.1055/s-0032-1328301

Supporting information for: [https://doi.org/10.1055/s-0032-1328301]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/1630