75 research outputs found
Microkelvin thermometry with Bose-Einstein condensates of magnons and applications to studies of the AB interface in superfluid He
Coherent precession of trapped Bose-Einstein condensates of magnons is a
sensitive probe for magnetic relaxation processes in superfluid 3He-B down to
the lowest achievable temperatures. We use the dependence of the relaxation
rate on the density of thermal quasiparticles to implement thermometry in 3He-B
at temperatures below 300 K. Unlike popular vibrating wire or quartz
tuning fork based thermometers, magnon condensates allow for contactless
temperature measurement and make possible an independent in situ determination
of the residual zero-temperature relaxation provided by the radiation damping.
We use this magnon-condensate-based thermometry to study the thermal impedance
of the interface between A and B phases of superfluid 3He. The magnon
condensate is also a sensitive probe of the orbital order-parameter texture.
This has allowed us to observe for the first time the non-thermal signature of
the annihilation of two AB interfaces.Comment: 26 pages, 7 figures, manuscript prepared for EU Microkelvin
Collaboration Workshop 2013. Accepted for publication in Journal of Low
Temperature Physic
Vortex-mediated relaxation of magnon BEC into light Higgs quasiparticles
A magnon Bose-Einstein condensate (BEC) in superfluid 3He is a fine instrument for studying the surrounding macroscopic quantum system. At zero temperature, the BEC is subject to a few distinct forms of decay into other collective excitations, owing to momentum and energy conservation in a quantum vacuum. We study the vortex-Higgs mechanism: The vortices relax the requirement for momentum conservation, allowing the optical magnons of the BEC to transform into light Higgs quasiparticles. This facilitates a direct measurement of the dimensions of the B-phase double-core vortex, providing experimental access to elusive phenomena, such as the Kelvin wave cascade and core-bound Majorana fermions. Our paper expands the spectrum of possible interactions between magnetic quasiparticles in 3He-BΒ and lays the groundwork for building magnon-based quantum devices
Rotating quantum wave turbulence
Turbulence under strong influence of rotation is described as an ensemble of interacting inertial waves across a wide range of length scales. In macroscopic quantum condensates, the quasiclassical turbulent dynamics at large scales is altered at small scales, where the quantization of vorticity is essential. The nature of this transition remains an unanswered question. Here we expand the concept of wave-driven turbulence to rotating quantum fluids where the spectrum of waves extends to microscopic scales as Kelvin waves on quantized vortices. We excite inertial waves at the largest scale by periodic modulation of the angular velocity and observe dissipation-independent transfer of energy to smaller scales and the eventual onset of the elusive Kelvin wave cascade at the lowest temperatures. We further find that energy is pumped to the system through a boundary layer distinct from the classical Ekman layer and support our observations with numerical simulations. Our experiments demonstrate a regime of turbulent motion in quantum fluids where the role of vortex reconnections can be neglected, thus stripping the transition between the classical and the quantum regimes of turbulence down to its constituent components
Quasiparticle transport in a two-dimensional boundary superfluid
The B phase of superfluid 3He can be cooled into the "pure" superfluid
regime, characterised by negligible thermal quasiparticle density. Here, the
bulk superfluid is bounded by a two-dimensional quantum well at the boundaries
of the container, where creating quasiparticles requires much less energy. In
this Article, we carry out experiments where we create a non-equilibrium state
within the quantum well and show that the induced quasiparticle currents flow
diffusively in the two-dimensional system. We conclude that the bulk of
superfluid 3He is wrapped by an independent two-dimensional superfluid that
interacts with mechanical probes instead of the bulk superfluid, only providing
access to the bulk superfluid if given a sudden burst of energy. That is,
superfluid 3He at the lowest temperatures and applied energies is
thermo-mechanically two dimensional. Our work opens this two-dimensional
quantum condensate and the interface it forms between the observer and the bulk
superfluid for exploration, and provides the possibility of creating
two-dimensional condensates of arbitrary topology.Comment: 11 pages, 9 figure
Apoptosis in the liver of male <em>db/db</em> mice during the development of obesity and type 2 diabetes
Obesity and diabetes mellitus are known to lead to the development of metabolic syndrome and non-alcoholic fatty liver disease (NAFLD). The mechanisms of programmed cell death are actively involved in maintaining cellular homeostasis along development of NAFLD. Proteins of the BCL-2 family are key regulators of physiological and pathological apoptosis. Homozygous males of BKS.Cg-Dock7mLeprdb/+/+/J mice (db/db mice) are characterized by progressive obesity and the development of type 2 diabetes mellitus (DM2) with severe hyperglycemia at 4β8 weeks and organ lesions at 8β10 weeks of age. The aim of this research was to study the expression of molecular cell regulators of apoptosis in liver cells of db/db mice males at different stages of obesity and diabetes development (at the age of 10 and 18 weeks). Immunohistochemical analysis (using the indirect avidin-biotin peroxidase method) and morphometric evaluation of the expression of the antiapoptotic protein Bcl-2 and the proapoptotic protein Bad in liver cells of studied animals at different stages of obesity and DM2 were carried out. An excess of the value of the Bcl-2 protein staining area over the Bad protein staining area was revealed in the liver of 10-week-old animals. The Bcl-2/Bad expression area ratio in 10-week-old animals was twice as high as in 18-week-old animals, which indicates the presence of conditions for blocking apoptosis in the liver of younger db/db mice. At the 18th week of life, db/db mice displayed an almost threefold increase in the expression area of the Bad protein against the background of an unchanged expression of the Bcl-2 protein. The decrease in the Bcl-2/Bad staining area ratio in 18-week-old animals was due to the increase in the Bad expression area, which indicates the absence of antiapoptotic cell protection and creates conditions for activation of the mitochondrial pathway of apoptosis in the liver of male db/db mice with pronounced signs of obesity and DM2
ΠΠΏΡΠΈΠΌΠΈΠ·Π°ΡΠΈΡ ΡΠΎΡΠΌΡ ΡΠΈΠ³Π½Π°Π»ΠΎΠ² Ρ ΠΊΠ²Π°Π΄ΡΠ°ΡΡΡΠ½ΠΎΠΉ Π°ΠΌΠΏΠ»ΠΈΡΡΠ΄Π½ΠΎΠΉ ΠΌΠΎΠ΄ΡΠ»ΡΡΠΈΠ΅ΠΉ Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΠΊΡΠΈΡΠ΅ΡΠΈΡ Π·Π°Π΄Π°Π½Π½ΠΎΠΉ ΡΠΊΠΎΡΠΎΡΡΠΈ ΡΠΏΠ°Π΄Π° ΡΡΠΎΠ²Π½Ρ Π²Π½Π΅ΠΏΠΎΠ»ΠΎΡΠ½ΡΡ ΠΈΠ·Π»ΡΡΠ΅Π½ΠΈΠΉ
Introduction. The growth in the volume of information transmitted through communication channels leads to their significant congestion. Almost all methods conventionally used to increase the data transfer rate in given frequency bands have been exhausted. In this regard, it is of interest to use new approaches aimed at improving the spectral efficiency of radio engineering and telecommunication systems by applying optimal signals that make it possible to use the bandwidth reserves of transmission channels given by Shannon's theory.Aim. Improvement of the spectral efficiency of digital messaging systems by using signals with a compact spectrum and increased volume of the channel alphabet at the same time as minimizing energy losses.Materials and methods. When searching for optimal signals, the mathematical apparatus of communication theory and functional analysis, as well as the methods of calculus of variations, were used. The evaluation of bit error rate performance of the obtained optimal signals transmitted in a channel with additive white Gaussian noise was performed in the MatLab environment. Results. It was established that, in a channel with additive white Gaussian noise, optimal signals with a signal constellation size of 64 in the case of quadrature amplitude-phase modulation provide an energy gain of more than 1 dB relative to signals obtained by narrowband filtering under the conditions of uncontrolled intersymbol interference. Optimal signals were shown to provide for an energy gain of 4.9 dB with respect to signals based on narrow-band filtering at a fixed spectral efficiency of 7 (bit/s)/Hz.Conclusion. The paper proposes a method for improving the spectral efficiency of quadrature signals with amplitudephase modulation, based on the use of optimal pulse shapes obtained as a result of solving an optimization problem. The optimization problem was solved according to the criterion of minimizing out-of-band emissions with the constraint on bit error rate performance in case of amplitude-phase modulation. The energy and spectral efficiency of signals with optimal pulse shapes and signals based on narrow-band filtering were compared.ΠΠ²Π΅Π΄Π΅Π½ΠΈΠ΅. Π ΠΎΡΡ ΠΎΠ±ΡΠ΅ΠΌΠΎΠ² ΠΏΠ΅ΡΠ΅Π΄Π°Π²Π°Π΅ΠΌΠΎΠΉ ΠΈΠ½ΡΠΎΡΠΌΠ°ΡΠΈΠΈ ΠΏΠΎ ΠΊΠ°Π½Π°Π»Π°ΠΌ ΡΠ²ΡΠ·ΠΈ ΠΏΡΠΈΠ²ΠΎΠ΄ΠΈΡ ΠΊ ΠΈΡ
ΡΡΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠΉ ΠΏΠ΅ΡΠ΅Π³ΡΡΠΆΠ΅Π½Π½ΠΎΡΡΠΈ. ΠΡΠ°ΠΊΡΠΈΡΠ΅ΡΠΊΠΈ Π²ΡΠ΅ ΠΈΡΠΏΠΎΠ»ΡΠ·ΡΠ΅ΠΌΡΠ΅ ΡΡΠ°Π΄ΠΈΡΠΈΠΎΠ½Π½ΡΠ΅ ΠΌΠ΅ΡΠΎΠ΄Ρ ΠΏΠΎΠ²ΡΡΠ΅Π½ΠΈΡ ΡΠΊΠΎΡΠΎΡΡΠΈ ΠΏΠ΅ΡΠ΅Π΄Π°ΡΠΈ Π΄Π°Π½Π½ΡΡ
Π² Π·Π°Π΄Π°Π½Π½ΡΡ
ΠΏΠΎΠ»ΠΎΡΠ°Ρ
ΡΠ°ΡΡΠΎΡ ΠΈΡΡΠ΅ΡΠΏΠ°Π½Ρ. Π ΡΡΠΎΠΉ ΡΠ²ΡΠ·ΠΈ ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»ΡΠ΅Ρ ΠΈΠ½ΡΠ΅ΡΠ΅Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ Π½ΠΎΠ²ΡΡ
ΠΏΠΎΠ΄Ρ
ΠΎΠ΄ΠΎΠ², Π½Π°ΠΏΡΠ°Π²Π»Π΅Π½Π½ΡΡ
Π½Π° ΠΏΠΎΠ²ΡΡΠ΅Π½ΠΈΠ΅ ΡΠΏΠ΅ΠΊΡΡΠ°Π»ΡΠ½ΠΎΠΉ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΡΠ°Π΄ΠΈΠΎΡΠ΅Ρ
Π½ΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΈ ΡΠ΅Π»Π΅ΠΊΠΎΠΌΠΌΡΠ½ΠΈΠΊΠ°ΡΠΈΠΎΠ½Π½ΡΡ
ΡΠΈΡΡΠ΅ΠΌ ΠΏΡΡΠ΅ΠΌ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΡ ΠΎΠΏΡΠΈΠΌΠ°Π»ΡΠ½ΡΡ
ΡΠΈΠ³Π½Π°Π»ΠΎΠ², ΠΏΠΎΠ·Π²ΠΎΠ»ΡΡΡΠΈΡ
Π·Π°Π΄Π΅ΠΉΡΡΠ²ΠΎΠ²Π°ΡΡ ΡΠ΅Π·Π΅ΡΠ²Ρ ΠΏΡΠΎΠΏΡΡΠΊΠ½ΠΎΠΉ ΡΠΏΠΎΡΠΎΠ±Π½ΠΎΡΡΠΈ ΠΊΠ°Π½Π°Π»ΠΎΠ² ΠΏΠ΅ΡΠ΅Π΄Π°ΡΠΈ, ΠΊΠΎΡΠΎΡΡΠ΅ Π΄Π°Π΅Ρ ΡΠ΅ΠΎΡΠΈΡ Π¨Π΅Π½Π½ΠΎΠ½Π°.Π¦Π΅Π»Ρ ΡΠ°Π±ΠΎΡΡ. ΠΠΎΠ²ΡΡΠ΅Π½ΠΈΠ΅ ΡΠΏΠ΅ΠΊΡΡΠ°Π»ΡΠ½ΠΎΠΉ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΡΠΈΡΡΠ΅ΠΌ ΠΏΠ΅ΡΠ΅Π΄Π°ΡΠΈ ΡΠΈΡΡΠΎΠ²ΡΡ
ΡΠΎΠΎΠ±ΡΠ΅Π½ΠΈΠΉ ΠΏΡΡΠ΅ΠΌ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΡ ΡΠΈΠ³Π½Π°Π»ΠΎΠ² Ρ ΠΊΠΎΠΌΠΏΠ°ΠΊΡΠ½ΡΠΌ ΡΠΏΠ΅ΠΊΡΡΠΎΠΌ ΠΈ ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΡ ΠΎΠ±ΡΠ΅ΠΌΠ° ΠΊΠ°Π½Π°Π»ΡΠ½ΠΎΠ³ΠΎ Π°Π»ΡΠ°Π²ΠΈΡΠ° ΠΏΡΠΈ ΠΌΠΈΠ½ΠΈΠΌΠΈΠ·Π°ΡΠΈΠΈ ΡΠ½Π΅ΡΠ³Π΅ΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΏΠΎΡΠ΅ΡΡ.ΠΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ. ΠΡΠΈ ΠΏΠΎΠΈΡΠΊΠ΅ ΠΎΠΏΡΠΈΠΌΠ°Π»ΡΠ½ΡΡ
ΡΠΈΠ³Π½Π°Π»ΠΎΠ² ΠΈΡΠΏΠΎΠ»ΡΠ·ΡΠ΅ΡΡΡ ΠΌΠ°ΡΠ΅ΠΌΠ°ΡΠΈΡΠ΅ΡΠΊΠΈΠΉ Π°ΠΏΠΏΠ°ΡΠ°Ρ ΡΠ΅ΠΎΡΠΈΠΈ ΡΠ²ΡΠ·ΠΈ ΠΈ ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π°, Π° ΡΠ°ΠΊΠΆΠ΅ ΠΌΠ΅ΡΠΎΠ΄Ρ Π²Π°ΡΠΈΠ°ΡΠΈΠΎΠ½Π½ΠΎΠ³ΠΎ ΠΈΡΡΠΈΡΠ»Π΅Π½ΠΈΡ. ΠΡΠ΅Π½ΠΊΠ° ΠΏΠΎΠΌΠ΅Ρ
ΠΎΡΡΡΠΎΠΉΡΠΈΠ²ΠΎΡΡΠΈ ΠΏΡΠΈΠ΅ΠΌΠ° ΠΏΠΎΠ»ΡΡΠ΅Π½Π½ΡΡ
ΠΎΠΏΡΠΈΠΌΠ°Π»ΡΠ½ΡΡ
ΡΠΈΠ³Π½Π°Π»ΠΎΠ² ΠΏΡΠΈ ΠΏΠ΅ΡΠ΅Π΄Π°ΡΠ΅ Π² ΠΊΠ°Π½Π°Π»Π΅ Ρ Π°Π΄Π΄ΠΈΡΠΈΠ²Π½ΡΠΌ Π±Π΅Π»ΡΠΌ Π³Π°ΡΡΡΠΎΠ²ΡΠΊΠΈΠΌ ΡΡΠΌΠΎΠΌ Π²ΡΠΏΠΎΠ»Π½Π΅Π½Π° Π² ΡΡΠ΅Π΄Π΅ MatLab.Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. Π£ΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ, ΡΡΠΎ Π² ΠΊΠ°Π½Π°Π»Π΅ Ρ Π°Π΄Π΄ΠΈΡΠΈΠ²Π½ΡΠΌ Π±Π΅Π»ΡΠΌ Π³Π°ΡΡΡΠΎΠ²ΡΠΊΠΈΠΌ ΡΡΠΌΠΎΠΌ ΠΎΠΏΡΠΈΠΌΠ°Π»ΡΠ½ΡΠ΅ ΡΠΈΠ³Π½Π°Π»Ρ ΠΏΡΠΈ ΡΠ°Π·ΠΌΠ΅ΡΠ΅ ΡΠΈΠ³Π½Π°Π»ΡΠ½ΠΎΠ³ΠΎ ΡΠΎΠ·Π²Π΅Π·Π΄ΠΈΡ 64 Π² ΡΠ»ΡΡΠ°Π΅ ΠΊΠ²Π°Π΄ΡΠ°ΡΡΡΠ½ΠΎΠΉ Π°ΠΌΠΏΠ»ΠΈΡΡΠ΄Π½ΠΎ-ΡΠ°Π·ΠΎΠ²ΠΎΠΉ ΠΌΠΎΠ΄ΡΠ»ΡΡΠΈΠΈ ΠΎΠ±Π΅ΡΠΏΠ΅ΡΠΈΠ²Π°ΡΡ ΡΠ½Π΅ΡΠ³Π΅ΡΠΈΡΠ΅ΡΠΊΠΈΠΉ Π²ΡΠΈΠ³ΡΡΡ Π±ΠΎΠ»Π΅Π΅ 1 Π΄Π ΠΎΡΠ½ΠΎΡΠΈΡΠ΅Π»ΡΠ½ΠΎ ΡΠΈΠ³Π½Π°Π»ΠΎΠ², ΠΊΠΎΡΠΎΡΡΠ΅ ΠΏΠΎΠ»ΡΡΠ°ΡΡΡΡ ΠΏΡΡΠ΅ΠΌ ΡΠ·ΠΊΠΎΠΏΠΎΠ»ΠΎΡΠ½ΠΎΠΉ ΡΠΈΠ»ΡΡΡΠ°ΡΠΈΠΈ Π² ΡΡΠ»ΠΎΠ²ΠΈΡΡ
Π½Π΅ΠΊΠΎΠ½ΡΡΠΎΠ»ΠΈΡΡΠ΅ΠΌΠΎΠΉ ΠΌΠ΅ΠΆΡΠΈΠΌΠ²ΠΎΠ»ΡΠ½ΠΎΠΉ ΠΈΠ½ΡΠ΅ΡΡΠ΅ΡΠ΅Π½ΡΠΈΠΈ. ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ ΠΎΠΏΡΠΈΠΌΠ°Π»ΡΠ½ΡΠ΅ ΡΠΈΠ³Π½Π°Π»Ρ ΠΏΠΎΠ·Π²ΠΎΠ»ΡΡΡ ΠΏΠΎΠ»ΡΡΠΈΡΡ ΡΠ½Π΅ΡΠ³Π΅ΡΠΈΡΠ΅ΡΠΊΠΈΠΉ Π²ΡΠΈΠ³ΡΡΡ 4.9 Π΄Π ΠΏΠΎ ΠΎΡΠ½ΠΎΡΠ΅Π½ΠΈΡ ΠΊ ΡΠΈΠ³Π½Π°Π»Π°ΠΌ, ΠΏΠΎΡΡΡΠΎΠ΅Π½Π½ΡΠΌ Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ ΡΠ·ΠΊΠΎΠΏΠΎΠ»ΠΎΡΠ½ΠΎΠΉ ΡΠΈΠ»ΡΡΡΠ°ΡΠΈΠΈ, ΠΏΡΠΈ ΡΠΈΠΊΡΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΉ ΡΠΏΠ΅ΠΊΡΡΠ°Π»ΡΠ½ΠΎΠΉ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ 7 (Π±ΠΈΡ/Ρ)/ΠΡ.ΠΠ°ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅. ΠΡΠ΅Π΄Π»ΠΎΠΆΠ΅Π½ ΠΌΠ΅ΡΠΎΠ΄ ΠΏΠΎΠ²ΡΡΠ΅Π½ΠΈΡ ΡΠΏΠ΅ΠΊΡΡΠ°Π»ΡΠ½ΠΎΠΉ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΠΊΠ²Π°Π΄ΡΠ°ΡΡΡΠ½ΡΡ
ΡΠΈΠ³Π½Π°Π»ΠΎΠ² Ρ Π°ΠΌΠΏΠ»ΠΈΡΡΠ΄Π½ΠΎ-ΡΠ°Π·ΠΎΠ²ΠΎΠΉ ΠΌΠΎΠ΄ΡΠ»ΡΡΠΈΠ΅ΠΉ, ΠΎΡΠ½ΠΎΠ²Π°Π½Π½ΡΠΉ Π½Π° ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠΈ ΠΎΠΏΡΠΈΠΌΠ°Π»ΡΠ½ΡΡ
ΡΠΎΡΠΌ ΠΈΠΌΠΏΡΠ»ΡΡΠΎΠ², ΠΏΠΎΠ»ΡΡΠ΅Π½Π½ΡΡ
Π² Ρ
ΠΎΠ΄Π΅ ΡΠ΅ΡΠ΅Π½ΠΈΡ ΠΎΠΏΡΠΈΠΌΠΈΠ·Π°ΡΠΈΠΎΠ½Π½ΠΎΠΉ Π·Π°Π΄Π°ΡΠΈ. ΠΡΠΈΠ²Π΅Π΄Π΅Π½Π° ΠΏΡΠΎΡΠ΅Π΄ΡΡΠ° ΡΠ΅ΡΠ΅Π½ΠΈΡ ΠΎΠΏΡΠΈΠΌΠΈΠ·Π°ΡΠΈΠΎΠ½Π½ΠΎΠΉ Π·Π°Π΄Π°ΡΠΈ ΠΏΠΎ ΠΊΡΠΈΡΠ΅ΡΠΈΡ ΠΌΠΈΠ½ΠΈΠΌΠΈΠ·Π°ΡΠΈΠΈ Π²Π½Π΅ΠΏΠΎΠ»ΠΎΡΠ½ΡΡ
ΠΈΠ·Π»ΡΡΠ΅Π½ΠΈΠΉ ΠΏΡΠΈ Π½Π°Π»ΠΈΡΠΈΠΈ ΠΎΠ³ΡΠ°Π½ΠΈΡΠ΅Π½ΠΈΡ Π½Π° ΠΏΠΎΠΌΠ΅Ρ
ΠΎΡΡΡΠΎΠΉΡΠΈΠ²ΠΎΡΡΡ ΠΏΡΠΈΠ΅ΠΌΠ° Π² ΡΠ»ΡΡΠ°Π΅ Π°ΠΌΠΏΠ»ΠΈΡΡΠ΄Π½ΠΎ-ΡΠ°Π·ΠΎΠ²ΠΎΠΉ ΠΌΠΎΠ΄ΡΠ»ΡΡΠΈΠΈ. ΠΡΠΏΠΎΠ»Π½Π΅Π½ΠΎ ΡΡΠ°Π²Π½Π΅Π½ΠΈΠ΅ ΡΠ½Π΅ΡΠ³Π΅ΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΈ ΡΠΏΠ΅ΠΊΡΡΠ°Π»ΡΠ½ΠΎΠΉ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ, ΠΎΠ±Π΅ΡΠΏΠ΅ΡΠΈΠ²Π°Π΅ΠΌΠΎΠΉ ΡΠΈΠ³Π½Π°Π»Π°ΠΌΠΈ Ρ ΠΎΠΏΡΠΈΠΌΠ°Π»ΡΠ½ΡΠΌΠΈ ΡΠΎΡΠΌΠ°ΠΌΠΈ ΠΈΠΌΠΏΡΠ»ΡΡΠΎΠ² ΠΈ ΡΠΈΠ³Π½Π°Π»Π°ΠΌΠΈ, ΠΏΠΎΡΡΡΠΎΠ΅Π½Π½ΡΠΌΠΈ Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ ΡΠ·ΠΊΠΎΠΏΠΎΠ»ΠΎΡΠ½ΠΎΠΉ ΡΠΈΠ»ΡΡΡΠ°ΡΠΈΠΈ
The expression of apoptosis-regulating proteins Bcl-2 and Bad in liver cells of C57Bl/6 mice under light-induced functional pinealectomy and after correction with melatonin
The presence of humans and animals under long-term continuous lighting leads to a suppression of melatonin synthesis, that is, to light-induced functional pinealectomy (LIFP), and the development of desynchronosis. To create LIFP, C57Bl/6 mice were kept under 24-hour lighting (24hL) for 14 days. The animals in the control group were kept under standard lighting conditions. In the next series of experiments, mice with LIFP received daily intragastrically either melatonin (1 mg/kg body weight in 200 ΞΌl of distilled water) or 200 ΞΌl of water as a placebo. The comparison group consisted of intact animals that received placebo under standard lighting conditions. Immunohistochemical analysis (using an indirect avidin-biotin peroxidase method) revealed the expression of the antiapoptotic protein Bcl-2 and the proapoptotic protein Bad in sinusoid liver cells (a heterogeneous population consisting of the endotheliocytes, Kupffer cells, Ito cells, and Pit cells) and in individual hepatocytes. The Bad expression area in the liver of LIFP mice increased 4 times against a background of the unchanged Bcl-2 expression area. Changes in the brightness (a parameter inversely proportional to the marker concentration) of Bad and Bcl-2 areas did not reach significance. Our results indicate a weakening of the antiapoptotic protection of liver cells of LIFP animals, which creates conditions for activation of the βmitochondrial branchβ of apoptosis. Melatonin treatment of LIFP mice resulted in a 3.3-fold increase in Bcl-2 expression area and a 2.7 % decrease in Bcl-2 region brightness compared with the experimental untreated group. Bad protein parameters were unreliable. Thus, melatonin treatment of animals cancels the effect of LIFP, restoring the Bcl-2 expression area and increasing this protein concentration, which indicates an increase in antiapoptotic protection and creates conditions for blocking the development of the βmitochondrial branchβ of apoptosis in liver cells
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