160 research outputs found

    ORIGIN OF MELTING ANOMALIES IN THE JAPAN-BAIKAL CORRIDOR OF ASIA AT THE LATEST GEODYNAMIC STAGE: EVOLUTION FROM THE MANTLE TRANSITION LAYER AND GENERATION BY LITHOSPHERIC TRANSTENSION

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    At the latest geodynamic stage that is characterized by forces and processes of the last 90 Ma the lithosphere of Asia has been reactivated due to four main force factors: 1) mantle melting anomalies, 2) subduction-related interaction between the Pacific plates and the continental eastern margin, 3) convergent interaction between India and the continental southern margin, and 4) quasiperiodic orbital variations of the Earth. The starting point of the latest geodynamic stage [Rasskazov, Chuvashova, 2013] is consistent with the change of the Earth’s rotation due to the resonant interaction of its orbit with the orbit of the Mars in the time interval of 87–85 Ma [Ma et al., 2017].At the latest geodynamic stage that is characterized by forces and processes of the last 90 Ma the lithosphere of Asia has been reactivated due to four main force factors: 1) mantle melting anomalies, 2) subduction-related interaction between the Pacific plates and the continental eastern margin, 3) convergent interaction between India and the continental southern margin, and 4) quasiperiodic orbital variations of the Earth. The starting point of the latest geodynamic stage [Rasskazov, Chuvashova, 2013] is consistent with the change of the Earth’s rotation due to the resonant interaction of its orbit with the orbit of the Mars in the time interval of 87–85 Ma [Ma et al., 2017]

    Π ΠΈΡ„Ρ‚Ρ‹, ΠΎΡ€ΠΎΠ³Π΅Π½Ρ‹, ΠΊΡ€Π°Ρ‚ΠΎΠ½Ρ‹ ΠΈ глобальная Ρ‚Π΅ΠΊΡ‚ΠΎΠ½ΠΈΠΊΠ°: Π²Π²Π΅Π΄Π΅Π½ΠΈΠ΅ Π² ΠΏΡ€ΠΎΠ±Π»Π΅ΠΌΡƒ

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    A key role in developing the Earth theory is played by comparative studies of orogens, rifts, and platforms in the equatorial, middle and high latitudes of Asia and the adjacent Arctic regions. The modern shape of the planet’s triaxial asymmetrical cardioid ellipsoid results from its latest (Late Phanerozoic) geodynamic evolution that began in Arctic and then commenced in Asia. At this stage, mechanisms of the lithosphere extension and compression, combined with extension, were launched in Arctic and Asia, respectively. The special issue of Geodynamics & Tectonophysics presents papers on this topic.Π’ Ρ€Π°Π·Π²ΠΈΡ‚ΠΈΠΈ Ρ‚Π΅ΠΎΡ€ΠΈΠΈ Π—Π΅ΠΌΠ»ΠΈ ΠΊΠ»ΡŽΡ‡Π΅Π²ΡƒΡŽ Ρ€ΠΎΠ»ΡŒ ΠΈΠ³Ρ€Π°ΡŽΡ‚ ΡΡ€Π°Π²Π½ΠΈΡ‚Π΅Π»ΡŒΠ½Ρ‹Π΅ исслСдования ΠΎΡ€ΠΎΠ³Π΅Π½ΠΎΠ², Ρ€ΠΈΡ„Ρ‚ΠΎΠ² ΠΈ ΠΏΠ»Π°Ρ‚Ρ„ΠΎΡ€ΠΌ Π² ΡΠΊΠ²Π°Ρ‚ΠΎΡ€ΠΈΠ°Π»ΡŒΠ½Ρ‹Ρ…, ΡƒΠΌΠ΅Ρ€Π΅Π½Π½Ρ‹Ρ… ΠΈ высоких ΡˆΠΈΡ€ΠΎΡ‚Π°Ρ… Азии ΠΈ ΡΠΎΠΏΡ€Π΅Π΄Π΅Π»ΡŒΠ½ΠΎΠΉ Арктики. БоврСмСнная Ρ„ΠΎΡ€ΠΌΠ° трСхосного асиммСтричного ΠΊΠ°Ρ€Π΄ΠΈΠΎΠΈΠ΄Π°Π»ΡŒΠ½ΠΎΠ³ΠΎ эллипсоида Π½Π° ΠΏΠ»Π°Π½Π΅Ρ‚Π΅ обусловлСна Π΅Π΅ новСйшСй (позднСфанСрозойской) гСодинамичСской ΡΠ²ΠΎΠ»ΡŽΡ†ΠΈΠ΅ΠΉ, Π½Π°Ρ‡Π°Π²ΡˆΠ΅ΠΉΡΡ Π² АрктикС, Π° Π·Π°Ρ‚Π΅ΠΌ – Π² Азии. На новСйшСм гСодинамичСском этапС Π² АрктикС Π±Ρ‹Π» Π·Π°ΠΏΡƒΡ‰Π΅Π½ ΠΌΠ΅Ρ…Π°Π½ΠΈΠ·ΠΌ растяТСния литосфСры, Π² Азии – ΠΌΠ΅Ρ…Π°Π½ΠΈΠ·ΠΌ сТатия, ΡΠΎΡ‡Π΅Ρ‚Π°ΡŽΡ‰Π΅Π³ΠΎΡΡ с растяТСниСм. CΡ‚Π°Ρ‚ΡŒΠΈ ΠΏΠΎ этой Ρ‚Π΅ΠΌΠ°Ρ‚ΠΈΠΊΠ΅ прСдставлСны Π² ΡΠΏΠ΅Ρ†ΠΈΠ°Π»ΡŒΠ½ΠΎΠΌ выпускС ΠΆΡƒΡ€Π½Π°Π»Π° Β«Π“Π΅ΠΎΠ΄ΠΈΠ½Π°ΠΌΠΈΠΊΠ° ΠΈ Ρ‚Π΅ΠΊΡ‚ΠΎΠ½ΠΎΡ„ΠΈΠ·ΠΈΠΊΠ°Β»

    Investigation the role of religious organizations in system of general education: forms of state- confessional interaction

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    The purpose of the this was aimed at conducting a system analysis of the forms of interaction between religious organizations and modern states implementing the secular model regarding the regulation of religion component in the field of general educatio

    Models of the organization of the judicial system: the experience of Russia and foreign countries

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    This article provides an overview of the organization of the judiciary in various countries. Firstly, attention is drawn to the legislative framework on the basis of which the system of courts in a particular state is built. Secondly, the conclusion is drawn that there are three models of the organization of the judiciary: decen-tralized; moderately centralized; strongly centralized bathroom. Examples of states in which distinguished models of the organization of the judiciary operate are given. Particular attention is paid to the place of the Russian model in the classification of judicial systems according to the degree of centralization of the judiciar

    ΠŸΠ•Π Π˜ΠžΠ”Π˜Π—ΠΠ¦Π˜Π― Π‘ΠžΠ’Π Π•ΠœΠ•ΠΠΠ«Π₯ И ΠŸΠžΠ—Π”ΠΠ•ΠŸΠ›Π•Π™Π‘Π’ΠžΠ¦Π•Π-Π“ΠžΠ›ΠžΠ¦Π•ΠΠžΠ’Π«Π₯ Π“Π•ΠžΠ”Π˜ΠΠΠœΠ˜Π§Π•Π‘ΠšΠ˜Π₯ И ΠŸΠΠ›Π•ΠžΠšΠ›Π˜ΠœΠΠ’Π˜Π§Π•Π‘ΠšΠ˜Π₯ ΠŸΠ ΠžΠ¦Π•Π‘Π‘ΠžΠ’

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    Based on comparative analyses of spatial and temporal patterns of high- and medium-potassic basaltic eruptions in the Central Mongolia and marine survey records of Sr isotopes, it is revealed that the start of the recent geodynamic stage in the Central Mongolia correlates with the starting point of its global manifestation, which gives an evidence of a close relationship between magmatic occurrences in the region under study and processes of global convergence. The magmatic occurrences are considered as representing the recent geodynamic evolution of the past 90 Ma with milestones of ~66, 40–37, ~32 and 17–15 Ma ago. Global changes, except those ~32 Ma ago, are shown in marine records of Sr isotopes. The Late Plestocene – Holocene natural and climate setting is reconstructed from radiocarbon datings of various geological and paleobiological objects. Changes of the natural environment and climate of the Northern hemisphere are plotted with account of strong magma eruptions, attacks of asteroids and meteorites, changes of lithological compositions of sedimentary complexes and species compositions of fauna at the given time interval.Β Β ΠŸΡ€ΠΈΠ²Π΅Π΄Π΅Π½Ρ‹ Ρ€Π΅Π·ΡƒΠ»ΡŒΡ‚Π°Ρ‚Ρ‹ исслСдований, Π²Ρ‹ΠΏΠΎΠ»Π½Π΅Π½Π½Ρ‹Ρ… Π² Π»Π°Π±ΠΎΡ€Π°Ρ‚ΠΎΡ€ΠΈΠΈ ΠΈΠ·ΠΎΡ‚ΠΎΠΏΠΈΠΈ ΠΈ Π³Π΅ΠΎΡ…Ρ€ΠΎΠ½ΠΎΠ»ΠΎΠ³ΠΈΠΈ ΠΈ Π»Π°Π±ΠΎΡ€Π°Ρ‚ΠΎΡ€ΠΈΠΈ кайнозоя Π˜Π—Πš БО РАН Π² Ρ€Π°ΠΌΠΊΠ°Ρ… ΠΏΡ€ΠΎΠ΅ΠΊΡ‚ΠΎΠ² БО РАН: ИМП β„– 77 Β«Π˜Π·ΡƒΡ‡Π΅Π½ΠΈΠ΅ закономСрностСй проявлСния опасных ΠΏΡ€ΠΈΡ€ΠΎΠ΄Π½Ρ‹Ρ… процСссов Π² историчСски ΠΎΠ±ΠΎΠ·Ρ€ΠΈΠΌΠΎΠΌ ΠΏΡ€ΠΎΡˆΠ»ΠΎΠΌ для Ρ€Π°Π·Ρ€Π°Π±ΠΎΡ‚ΠΊΠΈ основ ΠΏΡ€ΠΎΠ³Π½ΠΎΠ·Π° ΠΈΡ… повСдСния Π½Π° блиТайшиС дСсятилСтия» (РК 01201282598) ΠΈ ΠŸΡ€. VIII.69.1. Β«Π€Π°ΠΊΡ‚ΠΎΡ€Ρ‹, ΠΎΠΏΡ€Π΅Π΄Π΅Π»ΡΡŽΡ‰ΠΈΠ΅ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ срСды ΠΈ ΠΊΠ»ΠΈΠΌΠ°Ρ‚Π° Π¦Π΅Π½Ρ‚Ρ€Π°Π»ΡŒΠ½ΠΎΠΉ Азии Π² ΠΊΠ°ΠΉΠ½ΠΎΠ·ΠΎΠ΅Β». На основС ΡΡ€Π°Π²Π½ΠΈΡ‚Π΅Π»ΡŒΠ½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° пространствСнно-Π²Ρ€Π΅ΠΌΠ΅Π½Π½ΠΎΠ³ΠΎ распрСдСлСния высоко- ΠΈ ΡƒΠΌΠ΅Ρ€Π΅Π½Π½ΠΎ ΠΊΠ°Π»ΠΈΠ΅Π²Ρ‹Ρ… Π±Π°Π·Π°Π»ΡŒΡ‚ΠΎΠ²Ρ‹Ρ… ΠΈΠ·Π²Π΅Ρ€ΠΆΠ΅Π½ΠΈΠΉ Π² Π¦Π΅Π½Ρ‚Ρ€Π°Π»ΡŒΠ½ΠΎΠΉ Монголии ΠΈ морских записСй ΠΈΠ·ΠΎΡ‚ΠΎΠΏΠΎΠ² Sr выявлСно соотвСтствиС Π½Π°Ρ‡Π°Π»Π° новСйшСго гСодинамичСского этапа Π² Π¦Π΅Π½Ρ‚Ρ€Π°Π»ΡŒΠ½ΠΎΠΉ Монголии Ρ‚ΠΎΡ‡ΠΊΠ΅ отсчСта Π΅Π³ΠΎ глобального выраТСния, ΡΠ²ΠΈΠ΄Π΅Ρ‚Π΅Π»ΡŒΡΡ‚Π²ΡƒΡŽΡ‰Π΅Π΅ ΠΎ тСсной связи магматичСских событий Ρ€Π΅Π³ΠΈΠΎΠ½Π° с Π³Π»ΠΎΠ±Π°Π»ΡŒΠ½Ρ‹ΠΌΠΈ процСссами ΠΊΠΎΠ½Π²Π΅Ρ€Π³Π΅Π½Ρ†ΠΈΠΈ. Π­Ρ‚ΠΈ магматичСскиС события Ρ€Π°ΡΡΠΌΠ°Ρ‚Ρ€ΠΈΠ²Π°ΡŽΡ‚ΡΡ ΠΊΠ°ΠΊ ΠΏΡ€Π΅Π΄ΡΡ‚Π°Π²ΠΈΡ‚Π΅Π»ΡŒΠ½Ρ‹Π΅ для новСйшСй гСодинамичСской ΡΠ²ΠΎΠ»ΡŽΡ†ΠΈΠΈ литосфСры Π·Π° послСдниС 90 ΠΌΠ»Π½ Π»Π΅Ρ‚, Π² Ρ…ΠΎΠ΄Π΅ ΠΊΠΎΡ‚ΠΎΡ€ΠΎΠΉ ΠΎΠΏΡ€Π΅Π΄Π΅Π»Π΅Π½Π° ваТнСйшая Ρ€ΠΎΠ»ΡŒ Ρ€ΡƒΠ±Π΅ΠΆΠ΅ΠΉ ~66, 40–37, ~32 ΠΈ 17–15 ΠΌΠ»Π½ Π»Π΅Ρ‚ Π½Π°Π·Π°Π΄. ΠŸΡ€ΠΎΠΈΡΡ…ΠΎΠ΄ΠΈΠ²ΡˆΠΈΠ΅ Π³Π»ΠΎΠ±Π°Π»ΡŒΠ½Ρ‹Π΅ измСнСния, Π·Π° ΠΈΡΠΊΠ»ΡŽΡ‡Π΅Π½ΠΈΠ΅ΠΌ Ρ€ΡƒΠ±Π΅ΠΆΠ° ~32 ΠΌΠ»Π½ Π»Π΅Ρ‚ Π½Π°Π·Π°Π΄, Π·Π°ΠΏΠ΅Ρ‡Π°Ρ‚Π»Π΅Π½Ρ‹ Π² морских записях ΠΈΠ·ΠΎΡ‚ΠΎΠΏΠΎΠ² Sr. Из Π°Π½Π°Π»ΠΈΠ·Π° Ρ€Π°Π΄ΠΈΠΎΡƒΠ³Π»Π΅Ρ€ΠΎΠ΄Π½ΠΎΠ³ΠΎ датирования Ρ€Π°Π·Π»ΠΈΡ‡Π½Ρ‹Ρ… гСологичСских ΠΈ палСобиологичСских ΠΎΠ±ΡŠΠ΅ΠΊΡ‚ΠΎΠ² восстановлСна ΠΏΡ€ΠΈΡ€ΠΎΠ΄Π½ΠΎ-климатичСская обстановка ΠΏΠΎΠ·Π΄Π½Π΅Π³ΠΎ плСйстоцСна ΠΈ Π³ΠΎΠ»ΠΎΡ†Π΅Π½Π°. ΠŸΠΎΡΡ‚Ρ€ΠΎΠ΅Π½Π° кривая, ΠΎΡ‚Ρ€Π°ΠΆΠ°ΡŽΡ‰Π°Ρ измСнСния ΠΏΡ€ΠΈΡ€ΠΎΠ΄Π½ΠΎΠΉ срСды ΠΈ ΠΊΠ»ΠΈΠΌΠ°Ρ‚Π° Π‘Π΅Π²Π΅Ρ€Π½ΠΎΠ³ΠΎ ΠΏΠΎΠ»ΡƒΡˆΠ°Ρ€ΠΈΡ с ΡƒΡ‡Π΅Ρ‚ΠΎΠΌ проявлСния ΠΌΠΎΡ‰Π½Ρ‹Ρ… вулканичСских ΠΈΠ·Π²Π΅Ρ€ΠΆΠ΅Π½ΠΈΠΉ ΠΈ астСроидно-ΠΌΠ΅Ρ‚Π΅ΠΎΡ€ΠΈΡ‚Π½Ρ‹Ρ… Π°Ρ‚Π°ΠΊ, ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ литологичСского состава осадочных комплСксов ΠΈ Π²ΠΈΠ΄ΠΎΠ²ΠΎΠ³ΠΎ состава Ρ„Π°ΡƒΠ½Ρ‹ Π½Π° Π·Π°Π΄Π°Π½Π½ΠΎΠΌ Π²Ρ€Π΅ΠΌΠ΅Π½Π½ΠΎΠΌ ΠΈΠ½Ρ‚Π΅Ρ€Π²Π°Π»Π΅.Β 

    National interests of the USA and Russia: comparative legal analysis

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    This article is devoted to the comparative analysis of doctrinal approaches concerning the determination of national interests in the USA and Russia, their legal consolidation and implementation practice in modern conditions. The features of the North American and Russian approaches to the issues of strategic planning and the development of program documents in the field of national interest provision are established and systematize

    GeneNet in 2005

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    The GeneNet system is designed for collection and analysis of the data on gene and metabolic networks, signal transduction pathways and kinetic characteristics of elementary processes. In the past 2 years, the GeneNet structure was considerably improved: (i) the current version of the database is now implemented using ORACLE9i; (ii) the capacities to describe the structure of the protein complexes and the interactions between the units are increased; (iii) two tables with kinetic constants and more detailed descriptions of certain reactions were added; and (iv) a module for kinetic modeling was supplemented. The current SRS release of the GeneNet database contains 37 graphical maps of gene networks, as well as descriptions of 1766 proteins, 1006 genes, 241 small molecules and 3254 relationships between gene network units, and 552 kinetic constants. Information distributed between 16 interlinked tables was obtained by annotating 1980 journal publications. SRS release of the GeneNet database, the graphical viewer and the modeling section are available at http://wwwmgs.bionet.nsc.ru/mgs/gnw/genenet/

    НовСйшая Π³Π΅ΠΎΠ΄ΠΈΠ½Π°ΠΌΠΈΠΊΠ° Π¦Π΅Π½Ρ‚Ρ€Π°Π»ΡŒΠ½ΠΎΠΉ Азии: ΠΏΠ΅Ρ€Π²ΠΈΡ‡Π½Ρ‹Π΅ ΠΈ Π²Ρ‚ΠΎΡ€ΠΈΡ‡Π½Ρ‹Π΅ ΠΌΠ°Π½Ρ‚ΠΈΠΉΠ½Ρ‹Π΅ расплавныС Π°Π½ΠΎΠΌΠ°Π»ΠΈΠΈ Π² контСкстС ΠΎΡ€ΠΎΠ³Π΅Π½Π΅Π·Π°, Ρ€ΠΈΡ„Ρ‚ΠΎΠ³Π΅Π½Π΅Π·Π° ΠΈ двиТСния-взаимодСйствия литосфСрных ΠΏΠ»ΠΈΡ‚

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    A comprehensive model for deep dynamics in Asia has been developed from the data on the evolution of melting anomalies in the context of lithospheric plate motions, interactions, orogeny, and rifting. The key components of our model are the primary (transition layer) and secondary (upper mantle) melting anomalies (Gobi, Baikal, and North Transbaikalia; and Hangay, Sayan, and Vitim, respectively). It is inferred that the primary melting anomalies originated at the beginning of the latest geodynamic stage (ca. 90 Ma) as a result of the transition layer distortion by lower mantle flows. Such primary anomalies were caused by avalanche collapses of the slab material that had been stagnated under the closed fragments of the Solonker, Ural-Mongolian paleooceans and the Mongol-Okhotsk Bay of Paleopacific. The secondary melting anomalies occurred due to the Early-Middle Miocene structural reorganization in the Pacific-Asian and Indo-Asian interaction zones. The primary melting anomalies governed the spatial distribution of forces and processes of the latest geodynamic stage. The secondary melting anomalies resulted from the lithospheric motions relative to the primary anomalies and provided for the development of orogeny and rifting. The Baikal-Mongolian corridor of asthenospheric flows was limited by the lateral zones of convergent interactions between India and Asia in the southwest, and North America and Asia in the northeast. In these lateral zones, Late Phanerozoic paleoslabs and ascending mantle fluxes were revealed in the transition layer, as well as in the upper mantle, without any destruction by the asthenospheric flows.По Π΄Π°Π½Π½Ρ‹ΠΌ ΠΎΠ± ΡΠ²ΠΎΠ»ΡŽΡ†ΠΈΠΈ расплавных Π°Π½ΠΎΠΌΠ°Π»ΠΈΠΉ Π² контСкстС ΠΎΡ€ΠΎΠ³Π΅Π½Π΅Π·Π°, Ρ€ΠΈΡ„Ρ‚ΠΎΠ³Π΅Π½Π΅Π·Π°, двиТСния ΠΈ взаимодСйствия литосфСрных ΠΏΠ»ΠΈΡ‚ Ρ€Π°Π·Π²ΠΈΡ‚Π° ΠΎΠ±ΠΎΠ±Ρ‰Π°ΡŽΡ‰Π°Ρ модСль Π³Π»ΡƒΠ±ΠΈΠ½Π½ΠΎΠΉ Π΄ΠΈΠ½Π°ΠΌΠΈΠΊΠΈ Азии, Π² ΠΊΠΎΡ‚ΠΎΡ€ΠΎΠΉ Π²Π°ΠΆΠ½Π΅ΠΉΡˆΡƒΡŽ Ρ€ΠΎΠ»ΡŒ ΠΈΠ³Ρ€Π°Π»ΠΈ ΠΏΠ΅Ρ€Π²ΠΈΡ‡Π½Ρ‹Π΅ Гобийская, Π‘Π°ΠΉΠΊΠ°Π»ΡŒΡΠΊΠ°Ρ ΠΈ Π‘Π΅Π²Π΅Ρ€ΠΎ-Π—Π°Π±Π°ΠΉΠΊΠ°Π»ΡŒΡΠΊΠ°Ρ расплавныС Π°Π½ΠΎΠΌΠ°Π»ΠΈΠΈ ΠΏΠ΅Ρ€Π΅Ρ…ΠΎΠ΄Π½ΠΎΠ³ΠΎ слоя ΠΈ Π²Ρ‚ΠΎΡ€ΠΈΡ‡Π½Ρ‹Π΅ Π₯ангайская, Баянская ΠΈ Π‘Π΅Π²Π΅Ρ€ΠΎ-Π‘Π°ΠΉΠΊΠ°Π»ΡŒΡΠΊΠ°Ρ расплавныС Π°Π½ΠΎΠΌΠ°Π»ΠΈΠΈ Π²Π΅Ρ€Ρ…Π½Π΅ΠΉ ΠΌΠ°Π½Ρ‚ΠΈΠΈ. ΠŸΡ€Π΅Π΄ΠΏΠΎΠ»Π°Π³Π°Π΅Ρ‚ΡΡ, Ρ‡Ρ‚ΠΎ ΠΏΠ΅Ρ€Π²ΠΈΡ‡Π½Ρ‹Π΅ расплавныС Π°Π½ΠΎΠΌΠ°Π»ΠΈΠΈ Ρ„ΠΎΡ€ΠΌΠΈΡ€ΠΎΠ²Π°Π»ΠΈΡΡŒ Π² Π½Π°Ρ‡Π°Π»Π΅ новСйшСго гСодинамичСского этапа (ΠΎΠΊΠΎΠ»ΠΎ 90 ΠΌΠ»Π½ Π»Π΅Ρ‚ Π½Π°Π·Π°Π΄) Π² Ρ€Π΅Π·ΡƒΠ»ΡŒΡ‚Π°Ρ‚Π΅ Π½Π°Ρ€ΡƒΡˆΠ΅Π½ΠΈΡ ΠΏΠ΅Ρ€Π΅Ρ…ΠΎΠ΄Π½ΠΎΠ³ΠΎ слоя Π½ΠΈΠΆΠ½Π΅ΠΌΠ°Π½Ρ‚ΠΈΠΉΠ½Ρ‹ΠΌΠΈ ΠΏΠΎΡ‚ΠΎΠΊΠ°ΠΌΠΈ ΠΈΠ·-Π·Π° Π»Π°Π²ΠΈΠ½Π½ΠΎΠ³ΠΎ ΠΎΠ±Ρ€ΡƒΡˆΠ΅Π½ΠΈΡ слэбового ΠΌΠ°Ρ‚Π΅Ρ€ΠΈΠ°Π»Π°, ΠΊΠΎΡ‚ΠΎΡ€Ρ‹ΠΉ стагнировал ΠΏΠΎΠ΄ Π·Π°ΠΊΡ€Ρ‹Π²ΡˆΠΈΠΌΠΈΡΡ Ρ„Ρ€Π°Π³ΠΌΠ΅Π½Ρ‚Π°ΠΌΠΈ БолонкСрского, Π£Ρ€Π°Π»ΠΎ-Монгольского ΠΏΠ°Π»Π΅ΠΎΠΎΠΊΠ΅Π°Π½ΠΎΠ² ΠΈ Монголо-ΠžΡ…ΠΎΡ‚ΡΠΊΠΎΠ³ΠΎ Π·Π°Π»ΠΈΠ²Π° ΠŸΠ°Π»Π΅ΠΎΠΏΠ°Ρ†ΠΈΡ„ΠΈΠΊΠ°, Π° Π²Ρ‚ΠΎΡ€ΠΈΡ‡Π½Ρ‹Π΅ ΠΏΠΎΠ»ΡƒΡ‡ΠΈΠ»ΠΈ Ρ€Π°Π·Π²ΠΈΡ‚ΠΈΠ΅ Π² связи с процСссами раннСсрСднСмиоцСновой структурной пСрСстройки Π² Π·ΠΎΠ½Π°Ρ… ВихоокСанско-Азиатского ΠΈ Индо-Азиатского взаимодСйствия. ΠŸΠ΅Ρ€Π²ΠΈΡ‡Π½Ρ‹Π΅ расплавныС Π°Π½ΠΎΠΌΠ°Π»ΠΈΠΈ слуТили Π² качСствС Π³Π»Π°Π²Π½ΠΎΠ³ΠΎ Ρ„Π°ΠΊΡ‚ΠΎΡ€Π° пространствСнного распрСдСлСния усилий ΠΈ процСссов новСйшСго гСодинамичСского этапа, Π° Π²Ρ‚ΠΎΡ€ΠΈΡ‡Π½Ρ‹Π΅ явились слСдствиСм двиТСния литосфСры ΠΎΡ‚Π½ΠΎΡΠΈΡ‚Π΅Π»ΡŒΠ½ΠΎ ΠΏΠ΅Ρ€Π²ΠΈΡ‡Π½Ρ‹Ρ… Π°Π½ΠΎΠΌΠ°Π»ΠΈΠΉ ΠΈ способствовали Ρ€Π°Π·Π²ΠΈΡ‚ΠΈΡŽ ΠΎΡ€ΠΎΠ³Π΅Π½Π΅Π·Π° ΠΈ Ρ€ΠΈΡ„Ρ‚ΠΎΠ³Π΅Π½Π΅Π·Π°. Японско-Π‘Π°ΠΉΠΊΠ°Π»ΡŒΡΠΊΠΈΠΉ гСодинамичСский ΠΊΠΎΡ€ΠΈΠ΄ΠΎΡ€ сдСрТивался Π»Π°Ρ‚Π΅Ρ€Π°Π»ΡŒΠ½Ρ‹ΠΌΠΈ Π·ΠΎΠ½Π°ΠΌΠΈ ΠΊΠΎΠ½Π²Π΅Ρ€Π³Π΅Π½Ρ‚Π½ΠΎΠ³ΠΎ взаимодСйствия Π˜Π½Π΄ΠΎΡΡ‚Π°Π½Π° ΠΈ Азии Π½Π° юго-Π·Π°ΠΏΠ°Π΄Π΅ ΠΈ Π‘Π΅Π²Π΅Ρ€Π½ΠΎΠΉ АмСрики ΠΈ Азии Π½Π° сСвСро-востокС. Π’ Π»Π°Ρ‚Π΅Ρ€Π°Π»ΡŒΠ½Ρ‹Ρ… Π·ΠΎΠ½Π°Ρ… сдСрТивания позднСфанСрозойскиС палСослэбы ΠΈ восходящиС ΠΌΠ°Π½Ρ‚ΠΈΠΉΠ½Ρ‹Π΅ ΠΏΠΎΡ‚ΠΎΠΊΠΈ Π±Ρ‹Π»ΠΈ зафиксированы Π² ΠΏΠ΅Ρ€Π΅Ρ…ΠΎΠ΄Π½ΠΎΠΌ слоС ΠΈ Π²Π΅Ρ€Ρ…Π½Π΅ΠΉ ΠΌΠ°Π½Ρ‚ΠΈΠΈ Π±Π΅Π· Ρ€Π°Π·Ρ€ΡƒΡˆΠ΅Π½ΠΈΡ астСносфСрными ΠΏΠΎΡ‚ΠΎΠΊΠ°ΠΌΠΈ

    Isolation, characterization and molecular cloning of Duplex-Specific Nuclease from the hepatopancreas of the Kamchatka crab

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    <p>Abstract</p> <p>Background</p> <p>Nucleases, which are key components of biologically diverse processes such as DNA replication, repair and recombination, antiviral defense, apoptosis and digestion, have revolutionized the field of molecular biology. Indeed many standard molecular strategies, including molecular cloning, studies of DNA-protein interactions, and analysis of nucleic acid structures, would be virtually impossible without these versatile enzymes. The discovery of nucleases with unique properties has often served as the basis for the development of modern molecular biology methods. Thus, the search for novel nucleases with potentially exploitable functions remains an important scientific undertaking.</p> <p>Results</p> <p>Using degenerative primers and the rapid amplification of cDNA ends (RACE) procedure, we cloned the Duplex-Specific Nuclease (DSN) gene from the hepatopancreas of the Kamchatka crab and determined its full primary structure. We also developed an effective method for purifying functional DSN from the crab hepatopancreas. The isolated enzyme was highly thermostable, exhibited a broad pH optimum (5.5 – 7.5) and required divalent cations for activity, with manganese and cobalt being especially effective. The enzyme was highly specific, cleaving double-stranded DNA or DNA in DNA-RNA hybrids, but not single-stranded DNA or single- or double-stranded RNA. Moreover, only DNA duplexes containing at least 9 base pairs were effectively cleaved by DSN; shorter DNA duplexes were left intact.</p> <p>Conclusion</p> <p>We describe a new DSN from Kamchatka crab hepatopancreas, determining its primary structure and developing a preparative method for its purification. We found that DSN had unique substrate specificity, cleaving only DNA duplexes longer than 8 base pairs, or DNA in DNA-RNA hybrids. Interestingly, the DSN primary structure is homologous to well-known Serratia-like non-specific nucleases structures, but the properties of DSN are distinct. The unique substrate specificity of DSN should prove valuable in certain molecular biology applications.</p
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