Treatment of giant congenital melanocytic nevi with cultured epithelial autografts: Clinical and histopathological analysis

[Introduction] Curettage and dermabrasion are effective in the treatment of giant congenital melanocytic nevi (GCMN); however, local infection and hypertrophic scar formation are major issues. Thus, we applied cultured epithelial autografts (CEA) on skin defects after curettage or abrasion of GCMN and assessed the postoperative outcomes. [Methods] Seven nevi lesions of five patients (aged 3 months to 24 years) were treated with CEA after curettage or abrasion with a dermatome or a surgical bar, respectively. We assessed the postoperative outcomes, including CEA take ratio, erosion and/or ulcer formation in the acute phase, hospitalization days, Vancouver scar scale, and color improvement one year after the operation. In addition, a histological evaluation of a skin biopsy was performed over one year after the operation. [Results] The CEAs took well on the wound, and the wound surface was mostly epithelized by postoperative day 7 in all cases. While hypertrophic scar formation and slight pigmentation were observed in some lesions, the color was improved in all of the treated lesions. Histopathological examination revealed that the regenerated epidermis had stratified keratinocytes with rete ridges, and the dermal layer without nevus cells regenerated above the remaining dermis layer. [Conclusions] In this study, we found that early epithelialization and regeneration of the dermal layer was achieved after the application of CEA, suggesting that CEA could be an effective option after curettage or abrasion of GCMN

ALMA CO Observations of Supernova Remnant N63A in the Large Magellanic Cloud: Discovery of Dense Molecular Clouds Embedded within Shock-Ionized and Photoionized Nebulae

We carried out new $^{12}$CO($J$ = 1-0, 3-2) observations of a N63A supernova remnant (SNR) from the LMC using ALMA and ASTE. We find three giant molecular clouds toward the northeast, east, and near the center of the SNR. Using the ALMA data, we spatially resolved clumpy molecular clouds embedded within the optical nebulae in both the shock-ionized and photoionized lobes discovered by previous H$\alpha$ and [S II] observations. The total mass of the molecular clouds is $\sim$$800$ $M_{\odot}$ for the shock-ionized region and $\sim$$1700$ $M_{\odot}$ for the photoionized region. Spatially resolved X-ray spectroscopy reveals that the absorbing column densities toward the molecular clouds are $\sim$$1.5$-$6.0\times10^{21}$ cm$^{-2}$, which are $\sim$$1.5$-$15$ times less than the averaged interstellar proton column densities for each region. This means that the X-rays are produced not only behind the molecular clouds, but also in front of them. We conclude that the dense molecular clouds have been completely engulfed by the shock waves, but have still survived erosion owing to their high-density and short interacting time. The X-ray spectrum toward the gas clumps is well explained by an absorbed power-law or high-temperature plasma models in addition to the thermal plasma components, implying that the shock-cloud interaction is efficiently working for both the cases through the shock ionization and magnetic field amplification. If the hadronic gamma-ray is dominant in the GeV band, the total energy of cosmic-ray protons is calculated to be $\sim$$0.3$-$1.4\times10^{49}$ erg with the estimated ISM proton density of $\sim$$190\pm90$ cm$^{-3}$, containing both the shock-ionized gas and neutral atomic hydrogen.Comment: 18 pages, 4 tables, 8 figures, accepted for publication in The Astrophysical Journal (ApJ

Molecular Clouds associated with the Type Ia SNR N103B in the Large Magellanic Cloud

N103B is a Type Ia supernova remnant (SNR) in the Large Magellanic Cloud (LMC). We carried out new $^{12}$CO($J$ = 3-2) and $^{12}$CO($J$ = 1-0) observations using ASTE and ALMA. We have confirmed the existence of a giant molecular cloud (GMC) at $V_\mathrm{LSR}$ $\sim$245 km s$^{-1}$ towards the southeast of the SNR using ASTE $^{12}$CO($J$ = 3-2) data at an angular resolution of $\sim$25$"$ ($\sim$6 pc in the LMC). Using the ALMA $^{12}$CO($J$ = 1-0) data, we have spatially resolved CO clouds along the southeastern edge of the SNR with an angular resolution of $\sim$1.8$"$ ($\sim$0.4 pc in the LMC). The molecular clouds show an expanding gas motion in the position-velocity diagram with an expansion velocity of $\sim5$ km s$^{-1}$. The spatial extent of the expanding shell is roughly similar to that of the SNR. We also find tiny molecular clumps in the directions of optical nebula knots. We present a possible scenario that N103B exploded in the wind-bubble formed by the accretion winds from the progenitor system, and is now interacting with the dense gas wall. This is consistent with a single-degenerate scenario.Comment: 12 pages, 1 table, 8 figures, accepted for publication in The Astrophysical Journal (ApJ

Identification of effector candidate genes of Rhizoctonia solani AG-1 IA expressed during infection in Brachypodium distachyon

Rhizoctonia solani is a necrotrophic phytopathogen belonging to basidiomycetes. It causes rice sheath blight which inflicts serious damage in rice production. The infection strategy of this pathogen remains unclear. We previously demonstrated that salicylic acid-induced immunity could block R. solani AG-1 IA infection in both rice and Brachypodium distachyon. R. solani may undergo biotrophic process using effector proteins to suppress host immunity before necrotrophic stage. To identify pathogen genes expressed at the early infection process, here we developed an inoculation method using B. distachyon which enables to sample an increased amount of semi-synchronous infection hyphae. Sixty-one R. solani secretory effector-like protein genes (RsSEPGs) were identified using in silico approach with the publicly available gene annotation of R. solani AG-1 IA genome and our RNA-sequencing results obtained from hyphae grown on agar medium. Expression of RsSEPGs was analyzed at 6, 10, 16, 24, and 32 h after inoculation by a quantitative reverse transcription-polymerase chain reaction and 52 genes could be detected at least on a single time point tested. Their expressions showed phase-specific patterns which were classified into 6 clusters. The 23 RsSEPGs in the cluster 1-3 and 29 RsSEPGs in the cluster 4-6 are expected to be involved in biotrophic and necrotrophic interactions, respectively