1,906 research outputs found
No anomalous scaling in electrostatic calibrations for Casimir force measurements
In a recent paper (Phys.Rev.A78, 020101(R) (2008)), Kim at al. have reported
a large anomaly in the scaling law of the electrostatic interaction between a
sphere and a plate, which was observed during the calibration of their Casimir
force set-up. Here we experimentally demonstrate that in proper electrostatic
calibrations the scaling law follows the behavior expected from elementary
electrostatic arguments, even when the electrostatic voltage that one must
apply to minimize the force (typically ascribed to contact potentials) depends
on the separation between the surfaces.Comment: Final versio
Numerical modelling of liquid droplet dynamics in microgravity
Microgravity provides ideal experimental conditions for studying highly reactive and under-cooled materials where there is no contact between the sample and the other experimental apparatus. The non-contact conditions allow material properties to be measured from the oscillating liquid droplet response to perturbations. This work investigates the impact of a strong magnetic field on these measurement processes for weakly viscous, electrically conducting droplets. We present numerical results using an axisymmetric model that employs the pseudo-spectral collocation method and a recently developed 3D model. Both numerical models have been developed to solve the equations describing the coupled electromagnetic and fluid flow processes. The models represent the changing surface shape that results from the interaction between forces inside the droplet and the surface tension imposed boundary conditions. The models are used to examine the liquid droplet dynamics in a strong DC magnetic field. In each case the surface shape is decomposed into a superposition of spherical harmonic modes. The oscillation of the individual mode coefficients is then analysed to determine the oscillation frequencies and damping rates that are then compared to the low amplitude solutions predicted by the published analytical asymptotic theory
Fully Analyzing an Algebraic Polya Urn Model
This paper introduces and analyzes a particular class of Polya urns: balls
are of two colors, can only be added (the urns are said to be additive) and at
every step the same constant number of balls is added, thus only the color
compositions varies (the urns are said to be balanced). These properties make
this class of urns ideally suited for analysis from an "analytic combinatorics"
point-of-view, following in the footsteps of Flajolet-Dumas-Puyhaubert, 2006.
Through an algebraic generating function to which we apply a multiple
coalescing saddle-point method, we are able to give precise asymptotic results
for the probability distribution of the composition of the urn, as well as
local limit law and large deviation bounds.Comment: LATIN 2012, Arequipa : Peru (2012
Casimir-Polder interaction between an atom and a dielectric slab
We present an explicit analytic calculation of the energy-level shift of an
atom in front of a non-dispersive and non-dissipative dielectric slab. We work
with the fully quantized electromagnetic field, taking retardation into
account. We give the shift as a two-dimensional integral and use asymptotic
analysis to find expressions for it in various retarded and non-retarded
limiting cases. The results can be used to estimate the energy shift of an atom
close to layered microstructures.Comment: 10 pages, incl 7 figure
Counterion-Mediated Weak and Strong Coupling Electrostatic Interaction between Like-Charged Cylindrical Dielectrics
We examine the effective counterion-mediated electrostatic interaction
between two like-charged dielectric cylinders immersed in a continuous
dielectric medium containing neutralizing mobile counterions. We focus on the
effects of image charges induced as a result of the dielectric mismatch between
the cylindrical cores and the surrounding dielectric medium and investigate the
counterion-mediated electrostatic interaction between the cylinders in both
limits of weak and strong electrostatic couplings (corresponding, e.g., to
systems with monovalent and multivalent counterions, respectively). The results
are compared with extensive Monte-Carlo simulations exhibiting good agreement
with the limiting weak and strong coupling results in their respective regime
of validity.Comment: 19 pages, 10 figure
Cytosol- and clathrin-dependent stimulation of endocytosis in vitro by purified adaptors.
Using stage-specific assays for receptor-mediated endocytosis of transferrin (Tfn) into perforated A431 cells we show that purified adaptors stimulate coated pit assembly and ligand sequestration into deeply invaginated coated pits. Late events in endocytosis involving membrane fission and coated vesicle budding which lead to the internalization of Tfn are unaffected. AP2, plasma membrane adaptors, are active at physiological concentrations, whereas AP1, Golgi adaptors, are inactive. Adaptor-dependent stimulation of Tfn sequestration requires cytosolic clathrin, but is unaffected by clathrin purified from coated vesicles suggesting that soluble and assembled clathrin pools are functionally distinct. In addition to adaptors and cytosolic clathrin other, as yet unidentified, cytosolic factors are also required for efficient coated pit invagination. These results provide new insight into the mechanisms and regulation of coated pit assembly and invagination
Endocytosis Occurs Independently Of Annexin-Vi In Human A431 Cells
Annexin VI is one of a family of calcium-dependent phospholipid-binding proteins. Although the function of this protein is not known, various physiological roles have been proposed, including a role in the budding of clathrin-coated pits (Lin et al., 1992. Cell. 70:283-291.). In this study we have investigated a possible endocytotic role for annexin VI in intact cells, using the human squamous carcinoma cell line A431, and report that these cells do not express endogenous annexin VI, as judged by Western and Northern blotting and PCR/Southern blotting. To examine whether endocytosis might in some way be either facilitated or inhibited by the presence of annexin VI, a series of A431 clones were isolated in which annexin VI expression was achieved by stable transfection. These cells expressed annexin VI at similar levels to other human cell types. Using assays for endocytosis and recycling of the transferrin receptor, we report that each of these cellular processes occurs with identical kinetics in both transfected and wild-type A431 cells. In addition, purified annexin VI failed to support the scission of coated pits in permeabilized A431 cells. We conclude that annexin VI is not an essential component of the endocytic pathway, and that in A431 cells, annexin VI fails to exert any influence on internalization and recycling of the transferrin receptor
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