Resource allocation within the National AIDS Control Program of Pakistan: a qualitative assessment of decision maker's opinions

BACKGROUND: Limited resources, whether public or private, demand prioritisation among competing needs to maximise productivity. With a substantial increase in the number of reported HIV cases, little work has been done to understand how resources have been distributed and what factors may have influenced allocation within the newly introduced Enhanced National AIDS Control Program of Pakistan. The objective of this study was to identify perceptions of decision makers about the process of resource allocation within Pakistan's Enhanced National AIDS Control Program. METHODS: A qualitative study was undertaken and in-depth interviews of decision makers at provincial and federal levels responsible to allocate resources within the program were conducted. RESULTS: HIV was not considered a priority issue by all study participants and external funding for the program was thought to have been accepted because of poor foreign currency reserves and donor agency influence rather than local need. Political influences from the federal government and donor agencies were thought to manipulate distribution of funds within the program. These influences were thought to occur despite the existence of a well-laid out procedure to determine allocation of public resources. Lack of collaboration among departments involved in decision making, a pervasive lack of technical expertise, paucity of information and an atmosphere of ad hoc decision making were thought to reduce resistance to external pressures. CONCLUSION: Development of a unified program vision through a consultative process and advocacy is necessary to understand goals to be achieved, to enhance program ownership and develop consensus about how money and effort should be directed. Enhancing public sector expertise in planning and budgeting is essential not just for the program, but also to reduce reliance on external agencies for technical support. Strengthening available databases for effective decision making is required to make financial allocations based on real, rather than perceived needs. With a large part of HIV program funding dedicated to public-private partnerships, it becomes imperative to develop public sector capacity to administer contracts, coordinate and monitor activities of the non-governmental sector

Substantial and sustained reduction in under-5 mortality, diarrhea, and pneumonia in Oshikhandass, Pakistan : Evidence from two longitudinal cohort studies 15 years apart

Funding Information: Study 1 was funded through the Applied Diarrheal Disease Research Program at Harvard Institute for International Development with a grant from USAID (Project 936–5952, Cooperative Agreement # DPE-5952-A-00-5073-00), and the Aga Khan Health Service, Northern Areas and Chitral, Pakistan. Study 2 was funded by the Pakistan US S&T Cooperative Agreement between the Pakistan Higher Education Commission (HEC) (No.4–421/PAK-US/HEC/2010/955, grant to the Karakoram International University) and US National Academies of Science (Grant Number PGA-P211012 from NAS to the Fogarty International Center). The funding bodies had no role in the design of the study, data collection, analysis, interpretation, or writing of the manuscript. Publisher Copyright: © 2020 The Author(s).Peer reviewedPublisher PD

Growth performance, immune status and organ morphometry in broilers fed Bacillus subtilis-supplemented diet

The present research aimed to investigate the effects of Bacillus subtilis (B. subtilis) on performance, immune system, gut, and lymphoid organs’ microarchitecture in broilers. A total of 120 day-old broiler chicks were randomly distributed into four groups. The birds were fed a corn-soy-based basal diet (BD) (control) or the same BD supplemented with 10% zinc bacitracin (ZnB), 0.05 g/kg or 0.1 g/kg of B. subtilis (BS). The broilers fed 0.1 g/kg of B. subtilis had superior mean bodyweight and lower feed conversion ratio compared with the non-supplemented or ZnB-fed groups. The BS-0.1 group registered higher antibody titer against the Newcastle disease (ND) virus. Cell-mediated immune response post Phytohaemagglutinin-P (PHA-P) injection was attained by both BS-0.1 and BS-0.05 groups. Histomorphological study revealed increased thymus cortical width, and cortex/medulla ratio in BS-0.1 group compared with control. Area of bursal follicles and germinal centres of spleen also improved in BS-0.1 group. Compared to ZnB and control, higher villus height (VH) and villus crypt ratio of the duodenum and jejunum were recorded on day 21, and higher VH of duodenum and ileum was noted on day 35 in BS-0.1 and BS-0.05 groups. In conclusion, B. subtilis-type probiotics contributed positively to better growth performance, improved immune system and modulated morphology of lymphoid organs and gut mucosa in broilers.Keywords: Immunity, intestinal mucosa, poultry, probiotic

Effect of zinc and probiotics supplementation on performance and immune organs morphology in heat stressed broilers

The dietary supplementation of trace minerals and probiotics has long been practiced to reduce the harmful effects of high environmental temperature in poultry. The study aimed to assess the effect of single or combined supplementation of ZnSO4•H2O and probiotic (Protexin®) on the growth performance, gut biometry and immune organs histomorphology in broilers reared under cyclic heat stress. For this purpose, 280 day-old broiler chicks were randomly divided into seven groups designated as control (basal diet), HS (heat stress + basal diet), HS-Zn30 (HS + 30 mg ZnSO4•H2O/kg feed), HS-Zn60 (HS + 60 mg ZnSO4•H2O/kg feed), HS-Pro (HS + 0.1 g Protexin®/kg feed), HS-Zn30-Pro (HS + 30 mg ZnSO4•H2O/kg feed + 0.1 g Protexin®/kg feed), and HS-Zn60-Pro (HS + 60 mg ZnSO4•H2O/kg feed + 0.1 g Protexin®/kg feed). Each group consisted of five replicates (8 birds per replicate). The birds were fed on starter (0 - 21days) and grower (22 - 42 days) diets. From day 22 onwards, the birds were exposed to cyclic heat stress (temperature and relative humidity at 35 ± 1 °C and 75 ± 5% respectively) for 8 hours. The results revealed that birds exposed to heat stress showed significant decrease in body weight (BW) and increase in feed conversion ratio (FCR) compared to the control group. The dietary supplementation of ZnSO4•H2O and probiotic either singly or in combination improved BW and FCR throughout the experimental period in heat-stressed broilers. Area of lymphatic nodule and lymphoid follicle in caecal tonsils and bursa of Fabricius increased in HS-Pro, HS-Zn30-Pro and HS-Zn60-Pro groups compared to HS group. In conclusion, zinc and probiotics combination partially improved performance and immune organs histomorphology in heat-stressed broilers._Keywords: Chicken, heat stress, immune organ, morphology, Protexi

Effects of Ascorbic Acid and/or α-Tocopherol on Agronomic and Physio-Biochemical Traits of Oat (Avena sativa L.) under Drought Condition

Water stress is notably a critical environmental condition restricting plant growth and economic outputs in semi-arid and arid environments. In a pot experiment, we explored the potential function of α-tocopherol (α-toc) and/or ascorbic acid (AsA) on the agronomic and physio-biochemical features of oat grown in water-scarce conditions. Drought duration significantly reduced the soil electrical conductivity and pH but increased the soil temperature, influencing the nutrient availability and uptake. For example, post-drought (25 days) soil analysis indicated that electrical conductivity decreased from 597 to 306 mS/m, total dissolved solids from 298 to 153 mg/L, and pH from 7.5 to 6.3 in 25 days of drought. Further, the drought-stressed leaves also contained significantly lower metabolites, such as proline, protein, sugar, and glycine betaine, than the control leaves, indicating impaired plant defense mechanisms. Significantly increased enzymatic antioxidants in leaves (e.g., superoxide dismutase, ascorbate peroxidase, and peroxidase) suggested the inability of oat plants to overcome drought-induced oxidative damage. In contrast, AsA and/or α-toc significantly amplified the seed germination rates and plant growth. Taken together, our results demonstrate that AsA and α-toc have the capability to mitigate adverse effects of drought conditions on oat plants by improving leaf relative water contents, photosynthetic pigments, and the antioxidant defense system

A review on SAPO-34 zeolite materials for CO2 capture and conversion

Among several known zeolites, silicoaluminophosphate (SAPO)-34 zeolite exhibits a distinct chemical structure, unique pore size distribution, and chemical, thermal, and ion exchange capabilities, which have recently attracted considerable research attention. Global carbon dioxide (CO2) emissions are a serious environmental issue. Current atmospheric CO2 level exceeds 414 parts per million (ppm), which greatly influences humans, fauna, flora, and the ecosystem as a whole. Zeolites play a vital role in CO2 removal, recycling, and utilization. This review summarizes the properties of the SAPO-34 zeolite and its role in CO2 capture and separation from air and natural gas. In addition, due to their high thermal stability and catalytic nature, CO2 conversions into valuable products over single metal, bi-metallic, and tri-metallic catalysts and their oxides supported on SAPO-34 were also summarized. Considering these accomplishments, substantial problems related to SAPO-34 are discussed, and future recommendations are offered in detail to predict how SAPO-34 could be employed for greenhouse gas mitigation