Возможности ранней лабораторной диагностики клещевых нейроинфекций: клещевого энцефалита и Лайм-боррелиоза

Diagnostics of tick-borne encephalitis and Lyme borreliosis is possible only by laboratory confirmation. The purpose of the investigation was the study of possibilities of immunoferment analysis and polymerase chain reaction for early diagnostics of these infections. It was revealed that mixinfection of patient does not influence on revelation of infectious agent by immunoferment analysis and polymerase chain reaction in the early period of disease. For initial diagnostics of focal and meningeal forms of tick-born neuroinfections, revelation IgM in blood serum is the most informative. The investigation of patient liquor by the method of polymerase chain reaction is the most available for diagnostics of early neuroborreliosis. The combination of methods (immunoferment analysis and polymerase chain reaction) increases the effectiveness of early laboratory diagnostics of focal and meningeal forms of tick-born encephalitis and Lyme borreliosis.Необходимость лабораторного подтверждения клещевого энцефалита (КЭ) и Лайм-боррелиоза (ЛБ) не вызывает сомнения. Цель исследования — изучение возможностей иммуноферментного анализа (ИФА) и полимеразной цепной реакции (ПЦР) для ранней диагностики КЭ и ЛБ. В исследовании показано, что наличие микст-инфекции у больного не влияет на возможность выявления соответствующих возбудителей методами ИФА и ПЦР в раннем периоде заболевания. Для ранней лабораторной диагностики очаговых и менингеальных форм КЭ наиболее информативно выявление иммуноглобулина M в сыворотке крови. Наибольшую информативность для диагностики раннего нейроборрелиоза показало исследование ликвора больных методом ПЦР. Комбинация методов ИФА и ПЦР увеличивает эффективность ранней лабораторной диагностики очаговых и менингеальных форм КЭ и ЛБ

Opportunities of early laboratory diagnostics of tick-born neuroinfections: tick-borne encephalitis and Lyme borreliosis

Diagnostics of tick-borne encephalitis and Lyme borreliosis is possible only by laboratory confirmation. The purpose of the investigation was the study of possibilities of immunoferment analysis and polymerase chain reaction for early diagnostics of these infections. It was revealed that mixinfection of patient does not influence on revelation of infectious agent by immunoferment analysis and polymerase chain reaction in the early period of disease. For initial diagnostics of focal and meningeal forms of tick-born neuroinfections, revelation IgM in blood serum is the most informative. The investigation of patient liquor by the method of polymerase chain reaction is the most available for diagnostics of early neuroborreliosis. The combination of methods (immunoferment analysis and polymerase chain reaction) increases the effectiveness of early laboratory diagnostics of focal and meningeal forms of tick-born encephalitis and Lyme borreliosis.</jats:p