Determination of a correlation for predicting lean blow off limits of gaseous fueled, premixed turbulent jet flame arrays enclosed in a hexagonal dump combustor

Combustion of natural gas with air in gas turbines is a key technology for efficient provision of electric energy and heat. More stringent regulations regarding the emission of pollutants, such as NOx emissions, are necessitating research on technologies to reduce NOx formation during the combustion process. One technical approach onto the reduction of NOx-formation during combustion is fuel-lean premixed combustion. Current lean combustion concepts applied in stationary gas turbine combustors rely on flame stabilization through recirculation of hot flue gas using swirling flows. Swirl stabilized flames may be prone to combustion instabilities especially in lean premixed arrangements. Therefore, another approach is followed in the present study. In this concept, a matrix of turbulent lean premixed jet flames in a dump combustor is applied. The matrix burner consists of a nozzle with an array of circular channels in a hexagonal arrangement and a combustion chamber with a hexagonal cross section. In order to develop an appropriate burner design based on this concept, the experimental determination and theoretical evaluation of the lean blow out limit using different nozzles and operating conditions were conducted in this work in order to quantify the influence of different parameters on the flame stability. The varied geometric parameters are the diameter of the circular channels in the burner matrix as well as the ratio of the free cross section area of the nozzle to the cross section are of the combustion chamber, the combustor area dump ratio. The lean blow limit was determined at different preheating temperatures and flow velocities. The results show that the velocity at the LBO limit increases with increasing channel diameter, area combustor dump ratio and preheating temperature. The experimental results of three matrix burner are correlated in terms of a critical Damkoehler number and it is shown through experimental validation, that the Damkoehler number correlation derived is capable of predicting the LBO of a scaled matrix burner

Stabilization of bacterially expressed erythropoietin by single site-specific introduction of short branched PEG chains at naturally occurring glycosylation sites

The covalent attachment of polyethylene glycol (PEG) to therapeutic proteins can improve their physicochemical properties. In this work we utilized the non-natural amino acid p-azidophenylalanine (pAzF) in combination with the chemoselective Staudinger-phosphite reaction to install branched PEG chains to recombinant unglycosylated erythropoietin (EPO) at each single naturally occurring glycosylation site. PEGylation with two short 750 or 2000 Da PEG units at positions 24, 38, or 83 significantly decreased unspecific aggregation and proteolytic degradation while biological activity in vitro was preserved or even increased in comparison to full-glycosylated EPO. This site-specific bioconjugation approach permits to analyse the impact of PEGylation at single positions. These results represent an important step towards the engineering of site-specifically modified EPO variants from bacterial expression with increased therapeutic efficacy