PCR detection of Toxoplasma gondii B1 gene in women suffering from abortion

Background: Primary infection of maternal with toxoplasmosis during gestation and this infection transmission to the fetus continue to be the cause complex disease in offspring. Objective: This study was conducted to test the utility of nested Polymerase Chain Reaction (nPCR) assay to detect recent infections with Toxoplasma in abortive women. Material and methods: Toxoplasma gondii DNA was detected by using B1 gene as a target for amplification which was highly specific for T. gondii and is well conserved among all of the tested strains. Blood from 60 abortive women and 25 apparently healthy pregnant women with no history of abortion (as control group) were taken in this current study. Results: The results revealed that nPCR was positive in 48(80%) subjects and negative in 12(20%), Chi-square- χ2 for patients and control was ( 13.82 , 15.75 ) respectively. Conclusion: It can be concluded that nPCR assay in blood has advantage in detection of recent and active toxoplasmosis

Analysis of differential expression of hypoxia-inducible microRNA-210 gene targets in mild and severe preeclamptic patients

Preeclampsia (PE) is a multi-system disorder that is specific to human pregnancy. Inadequate oxygenation of uterus and placenta is considered as one of the leading causes for the disease. MicroRNA-210(miR-210) is one of the prime molecules that has emerged in response to hypoxia. The objective of this study was to determine miR-210 expression patterns in plasma from severe PE and mild PE patients, and how that affects the expression of miR-210 target genes. The expression levels of miR-210 were validated using reverse transcription-quantitative PCR in plasma of severe PE (15) and mild PE (15) patients in comparison to controls subjects (15) with normal pregnancy. Then, the association between miR-210 and its downstream genes was validated by using human miR-210 targets RT2 profiler PCR Array. Both the categories (mild and severe) showed significantly high miR-210 expression levels. Also out of the 84 hypoxia miR-210 associated genes screened using mRNA, 18 genes were found to be differentially expressed in severe PE whereas 16 genes in mild PE cases with varying magnitude. All the genes in both the PE groups were found downregulated in comparison to controls. These downregulated genes expressed in both the cases were shown to be participating in immunosuppression, apoptosis, cell growth, signaling, angiogenesis, DNA repair. This study provides novel data on the genes that work downstream of miR-210 and how dysregulated expression of miR-210 can affect their expression and in turn functioning which can be associated with PE risk and severity. This study is the very first to determine the effect of miR-210 expression levels on associated genes in plasma samples

Effet des venins de Macrovipera Lebetina et de Cerastes sur l'adhérence aux intégrines des cellules cancéreuse (IGR39, HT29-D4 et IGROV1)

International audienceIn this work, we provide experimental arguments in favor of the fact that components from Macrovipera lebetina and Cerastes cerastes venoms bind to IGR39 melanoma cells but not to HT29D4 cells that derive from carcinoma adenome. Furthermore, Macrovipera lebetina and Cerastes cerastes venoms inhibit the adherence of IGR39 and HT 29-D4 to various extracellular matrix proteins. Macrovipera lebetina and Cerastes cerastes venoms did not inhibit the non specific adherence of IGR 39 cells to polylysine. In addition, binding of components from Cerastes cerastes venom to IGR39 cells is inhibited by GRGDS peptide and by monoclonal antibidy anti-av, while these two components have no effect on the adherence of IGR39 to Macrovipera lebetina venom.Dans ce travail nous apportons des preuves expérimentales en faveur du fait que des composants du venin de Macrovipera lebetina et Cerastes ceraste sont capables de se fixer spécifiquement sur les cellules mélanomateuse IGR39 mais pas aux cellules HT29D4 dérivant d'un adénocarcinome

PCR detection of Toxoplasma gondii B1 gene in women suffering from abortion

Background: Primary infection of maternal with toxoplasmosis during gestation and this infection transmission to the fetus continue to be the cause complex disease in offspring. &#x0D; Objective: This study was conducted to test the utility of nested Polymerase Chain Reaction (nPCR) assay to detect recent infections with Toxoplasma in abortive women. &#x0D; Material and methods: Toxoplasma gondii DNA was detected by using B1 gene as a target for amplification which was highly specific for T. gondii and is well conserved among all of the tested strains. Blood from 60 abortive women and 25 apparently healthy pregnant women with no history of abortion (as control group) were taken in this current study.&#x0D; Results: The results revealed that nPCR was positive in 48(80%) subjects and negative in 12(20%), Chi-square- χ2 for patients and control was ( 13.82 , 15.75 ) respectively.&#x0D; Conclusion: It can be concluded that nPCR assay in blood has advantage in detection of recent and active toxoplasmosis.</jats:p

Effects of Widely Used Heavy Metals on Embryological Development of Model Organism Zebrafish (Danio rerio)

This study aimed to investigate the toxic effect of widely used heavy metals, which are KCN, CuSO4, K3Fe(CN)6, and FeClOH on the embryological development of model organism zebrafish (Danio rerio). Zebrafish embryos were exposed to these heavy metals at a concentration was 0.5 mg/L for 96 hpf (hours post-fertilization). Mortality, hatching, and malformation rates were examined during the exposure period. The result showed that FeClOH and K3Fe(CN)6 were toxic to zebrafish embryos and caused an increased mortality rate and delayed hatching. Moreover, FeClOH and K3Fe(CN)6 caused a lot of types of abnormalities while KCN and CuSO4 were more toxic to zebrafish embryos and caused a mortality rate, which was 100% at 24 hpf. In conclusion, this study showed that widely used heavy metals, such as KCN, CuSO4, FeCIOH, and K3Fe (CN)6 cause toxicity in embryos, delay hatching time and rate, and/or cause many different malformations.</jats:p