Development of two distinct dendritic-like APCs in the context of splenic stroma

10.3389/fimmu.2013.00073Frontiers in Immunology4MARarticle 7

Impact of the c-MybE308G mutation on mouse myelopoiesis and dendritic cell development

10.1371/journal.pone.0176345PLoS ONE124e017634

Antigen Presenting Properties of a Myeloid Dendritic-Like Cell in Murine Spleen

<div><p>This paper distinguishes a rare subset of myeloid dendritic-like cells found in mouse spleen from conventional (c) dendritic cells (DC) in terms of phenotype, function and gene expression. These cells are tentatively named “L-DC” since they resemble dendritic-like cells produced in longterm cultures of spleen. L-DC can be distinguished on the basis of their unique phenotype as CD11b^hiCD11c^loMHCII^-CD43⁺Ly6C^-Ly6G^-Siglec-F^- cells. They demonstrate similar ability as cDC to uptake and retain complex antigens like mannan via mannose receptors, but much lower ability to endocytose and retain soluble antigen. While L-DC differ from cDC by their inability to activate CD4⁺ T cells, they are capable of antigen cross-presentation for activation of CD8⁺ T cells, although less effectively so than the cDC subsets. In terms of gene expression, CD8^- cDC and CD8⁺ cDC are quite distinct from L-DC. CD8⁺ cDC are distinguishable from the other two subsets by expression of <i>CD24a</i>, <i>Clec9a</i>, <i>Xcr1</i> and <i>Tlr11</i>, while CD8^- cDC are distinguished by expression of <i>Ccnd1</i> and <i>H-2Eb2</i>. L-DC are distinct from the two cDC subsets through upregulated expression of <i>Clec4a3</i>, <i>Emr4</i>, <i>Itgam</i>, <i>Csf1r</i> and <i>CD300ld</i>. The L-DC gene profile is quite distinct from that of cDC, confirming a myeloid cell type with distinct antigen presenting properties.</p></div