The time-course evolution of viability and competence for proliferation of woody plant protoplasts following cold-storage

International audienc

Protoplast-to-tree systems for Prunus cerasifera and Prunus spinosa as a tool for phylogenetic studies within the Prunoideae, Rosaceae

International audienc

Organogenesis from leaf protoplasts of Passe Crassane and Old Home Pear (Pyrus communis L.) and the isoenzymatic assessment of the regenerated plants

International audienc

Flow cytometry enables identification of sporophytic eliciting stress treatments in gametic cells

International audienceFlow cytometry was used to quantify the effect of individual and combined stress treatments on elicitation of androgenesis by analyzing the relative nuclear DNA content of in vitro cultured microspores of Pisum sativum L. Differences in relative nuclear DNA content of microspores within anthers after stress treatments were clearly evident from the flow cytometry profiles, and permitted us to predict whether a combination of stresses were elicitors or enhancers of androgenesis. This is the first report to assess the effect of various stress treatments in a plant species based on relative nuclear DNA content and to use this information to categorize them as 'elicitors' or 'enhancers'. Flow cytometry represents a simple, quick and reliable way to analyze and discriminate the effect of various stress treatments on elicitation of androgenesis. These results form a solid basis for further efforts designed to enhance responses and to extend double haploid technology to other legume