An integrated process of biodiesel production from indigenous microalgae in Northern Greece: first results

For environmental, economic and competence reasons, the fuel industry has pushed for alternative fuels in the past few years. One option that is considered are biofuels, which are renewable fuels and have the characteristic of lower or zero emissions of CO2 in the lifecycle, depending on their origin, their production and use. That is done, because the carbon they contain has been committed in the development of organic matter from the atmosphere, which returns after combustion so the balance of emissions throughout the life cycle of biofuel is "theoretically zero

Platelet-Rich Plasma Guided Injections: Clinical Application in Peripheral Neuropathies

Platelet-Rich Plasma (PRP) is defined as an autologous concentrated preparation of platelets and their associated growth factors in a small volume of plasma. The presence of these growth factors has stimulated the scientific community to search about possible benefits of the use of PRP in tissue regeneration. Provided that previously in vitro and animal research demonstrated that PRP could probably play an important role in the treatment of neural tissue disorders, we aimed to review the current literature, regarding the clinical studies that have been conducted to confirm this hypothesis. More specifically, we have reviewed the literature concerning the clinical application of PRP in peripheral neuropathies and investigated if there is strong evidence to establish the use of PRP in clinical practice as a therapeutic option. In contrast with animal studies, we have been able to identify only few clinical data concerning the use of Platelet-Rich Plasma (PRP) in peripheral neuropathies. We found 5 trials matched to our research that have yield positive and promising results for the future for the application of PRP for the therapy of disorders of the peripheral nervous system. It is obvious that this interesting field of research gives to the scientists the ability to expand it extensively, in terms of both quality and quantity

Systemic hypertension augments, whereas insulin-dependent diabetes down-regulates, endothelin A receptor expression in the mammary artery in coronary artery disease patients

Background: Endothelin (ET) A receptor antagonism causes decreased vasodilation in hypertensive coronary arteries and decreased effects on coronary artery compliance in diabetic patients. Methods: We investigate the mRNA expression of ET-1, ETA and ETB receptors, using real time RT-PCR, in biopsies from the internal mammary artery obtained from 49 patients, 18 diabetics and 34 hypertensives, all undergoing coronary artery bypass grafting. Results: Hypertensive patients had higher ET-1 mRNA expression (16438 [8417, 23917]), than normotensive patients (2974 [2283, 18055], p=0.008). Diabetic patients had significantly lower ETA receptor levels than non-diabetic patients (455 [167, 1496] vs. 1660 [700, 3190], respectively, p = 0.003). Conclusions: Multivariate analysis demonstrated that the presence of systemic hypertension was the only independent predictor of log ETA receptor expression and log ET-1 expression, while insulin-dependent diabetes was negatively correlated with ETA receptor expression. ETB receptor expression was not correlated with any predictor. Systemic hypertension is associated with increased ET-1 and ETA receptor mRNA expression, whereas insulin-dependent diabetes down-regulates ETA receptor mRNA expression in the internal mammary artery in patients with coronary artery disease undergoing bypass grafting

Klotho and the treatment of human malignancies

Klotho was first discovered as an anti-ageing protein linked to a number of age-related disease processes, including cardiovascular, renal, musculoskeletal, and neurodegenerative conditions. Emerging research has also demonstrated a potential therapeutic role for Klotho in cancer biology, which is perhaps unsurprising given that cancer and ageing share similar molecular hallmarks. In addition to functioning as a tumour suppressor in numerous solid tumours and haematological malignancies, Klotho represents a candidate therapeutic target for patients with these diseases, the majority of whom have limited treatment options. Here, we examine contemporary evidence evaluating the anti-neoplastic effects of Klotho and describe the modulation of downstream oncogenic signalling pathways, including Wnt/β-catenin, FGF, IGF1, PIK3K/AKT, TGFβ, and the Unfolded Protein Response. We also discuss possible approaches to developing therapeutic Klotho and consider technological advances that may facilitate the delivery of Klotho through gene therapy

Recurrent intra-articular osteoid osteoma of the hip after radiofrequency ablation: a case report and review of the literature

We present a case of a 53-year-old woman with recurrent intra-articular osteoid osteoma of the hip 6 months after initial treatment with percutaneous radiofrequency ablation. En bloc surgical excision of the osteoid osteoma and prophylactic internal fixation for impending stress fracture was performed. The patient is pain free, has returned to normal function and there is no sign of recurrence at the one-year follow-up. Intraarticular osteoid osteoma, present a diagnostic challenge and often they are misdiagnosed. Minimally invasive ablation techniques can fail in significant percentage and then surgical excision with histological confirmation remains the definitive treatment of choice

MRI efficacy in diagnosing internal lesions of the knee: a retrospective analysis

BACKGROUND: Many surgeons tend to believe that MRI is an accurate, non invasive diagnostic method, enough to lead to decisions for conservative treatment and save a patient from unnecessary arthroscopy. We conducted a retrospective study to investigate the accuracy of the MRI of the knee for the detection of injuries of the meniscus, cruciate ligaments and articular cartilage, in comparison with the preoperative clinical examination and intraoperative findings. Between May 2005 and February 2006 102 patients after clinical examination were diagnosed with meniscal or cruciate injury and underwent definitive treatment with arthroscopy. 46 of these patients fulfilled the inclusion criteria. The accuracy, sensitivity, specificity, negative and positive predictive values of the MRI findings were correlated with the lesions identified during arthroscopy. The diagnostic performance of the initial clinical examination was also calculated for the meniscal and cruciate ligament injuries. RESULTS: The accuracy for tears of the medial, lateral meniscus, anterior and posterior cruciate ligaments and articular cartilage was 81%, 77%, 86%, 98% and 60% respectively. The specificity was 69%, 88%, 89%, 98% and 73% respectively. The positive predictive value was 83%, 81%, 90%, 75% and 53% respectively. Finally, the clinical examination had significant lower reliability in the detection of these injuries. CONCLUSION: MRI is very helpful in diagnosing meniscal and cruciate ligament injuries. But in a countable percentage reports with false results and in chondral defects its importance is still vague. The arthroscopy still remains the gold standard for definitive diagnosis

Properties and characterization of biodiesel from selected microalgea stains

The demand for alternative fuels has increased in the past several years[1]. Biofuels are gaining importance as significant substitutes for the depleting fossil fuels. The fact that biofuels are renewable fuels with very low emissions of CO2 in the lifecycle offers them a competitive advantage[2]. However, the first produced biodiesel derived from edible oil seed crops (first generation feedstocks), lurking a serious risk of disturbing the overall worldwide balance of food reserves and safety. The second generation feedstocks for biodiesel production obtained from non-edible oil seed crops, waste cooking oil, animal fats, etc., but these feedstocks are not sufficient to cover the present energy needs. Recent focus is on microalgae as the third generation feedstock[3]. Mi l d t t f l d b t th i lt ( ) b kih(l ) df h Microalgae do not compete for land, but they can grow in salty sea), brackish (lagoons) and fresh (lakes) water. Moreover, microalgae have high photosynthetic efficiency using solar energy, water and carbon dioxide to produce higher quantities of biomass than other feedstocks. In the present research work, two indigenous fresh water (ChlorF1, ChlorF2) and two marine (ChlorM1, ChlorM2) Chlorophyte strains have been cultivated successfully under laboratory conditions using commercial fertilizer (Nutrileaf 30-10-10, initial concentration=70 g/m3) as nutrient source. The produced biodiesel from the microalgae biomass achieved a range of 2.2 - 10.6% total lipid content and an unsaturated FAME content between 48 mol% and 59 mol%. The iodine value, the cetane number, the cold filter plugging point (CFPP) and the oxidative stability of the ultimate biodiesels were determined, based on the compositions of the four (4) microalgae strains and compared with the specifications in the EU and US standards, EN 14214 and ASTM D6751 respectively