650 research outputs found
DNA bending and orientation-dependent function of YY1 in the c-fos promoter
The assembly of multicomponent complexes at promoters, enhancers, and silencers likely entails perturbations in the path of the DNA helix. We present evidence that YY1, a ubiquitously expressed DNA-binding protein, regulates the activity of the c-fos promoter primarily through an effect on DNA structure. YY1 binds to and induces a phased DNA bend at three sites in this promoter. By use of a truncated c-fos promoter activity containing a single functional YY1 site, we show that YY1 represses promoter activity but that repression does not appear to be an intrinsic property of the protein in this context. Moreover, when the orientation of the YY1 site is reversed, YY1 activates the same promoter. Repression by YY1 is also alleviated by changing the relative phasing of factor-binding sites on either side of YY1. We conclude that the principal function of YY1 in this promoter is to bend DNA to regulate contact between other proteins. Thus, YY1 represents a new class of transcription factors that influences promoter function by affecting promoter structure rather than by directly contacting the transcriptional machinery. We provide evidence that the product of the male sex determination gene SRY may also belong to this class of structural factors
Mechanical property and biocompatibility of co-precipitated nano hydroxyapatite–gelatine composites
From Ceramics to Ceramic Steel: Genesis
Ceramics were introduced in dentistry since the 16th century as dental porcelain, primarily for the manufacture of porcelain teeth. Improved technology saw porcelain jacket crowns, metal ceramic systems with better bonding techniques of different alloys to porcelain, bonded foil systems and then the introduction of alumina and other oxides, such as zirconia tostrengthen ceramics. Superior processing methods and sintering technologies have lead to CAD/CAM ceramics and the all ceramic systems. This article provides an overview ofthe different methods of strengthening ceramics for a better understanding of the mechanisms involved, including that of the support system
Coastal Sediment Dynamics Around Netravati – Gurpur River Mouth Through Integrated Approach
Source: ICHE Conference Archive - https://mdi-de.baw.de/icheArchiv
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Oxidation behavior of molybdenum silicides and their composites
A key materials issue associated with the future of high-temperature structural silicides is the resistance of these materials to oxidation at low temperatures. Oxidation tests were conducted on Mo-based silicides over a wide temperature range to evaluate the effects of alloy composition and temperature on the protective scaling characteristics and testing regime for the materials. The study included Mo{sub 5}Si{sub 3} alloys that contained several concentrations of B. In addition, oxidation characteristics of MoSi{sub 2}-Si{sub 3}N{sub 4} composites that contained 20--80 vol.% Si{sub 3}N{sub 4} were evaluated at 500--1,400 C
Varietal identification and fingerprinting of Pearl Millet (Pennisetum glaucum L.) varieties and hybrid using morphological descriptors and SSR markers
Pearl Millet (Pennisetum glaucum) is the sixth most important cereal crop in the world. The genomic resources available in Pearl millet can be utilized for fingerprinting and screening of hybrids using SSR markers and will be helpful for the assessment of seed purity. Hence, the present study was focused on fingerprint popular pearl millet varieties and hybrids of Tamil Nadu for varietal identification and hybrid purity test. The varieties used for DNA fingerprinting were CO (Cu) 9, CO 10, Pearl Millet hybrid CO 9 along with the parents, A' line ICMA 93111A and R' line PT 6029-30. The morphological features were recorded to screen the cultivars. The Pearl millet hybrid CO 9 scored the highest value for more than four quantitative characters via., Number of productive tillers (4-6), Leaf blade length (60-68cm), Leaf blade width (4.0-4.5cm), number of nodes (8-10), and 1000 seed weight (13-14g) which is at par and comparable with the composite CO 10 and higher than that of the variety CO (Cu) 9. PCR was performed using 36 SSR primers to find out polymorphism among the varieties. The SSR markers ICMP3021 and PSMP2089 were able to selectively identify CO (Cu) 9 from the other varieties. Whereas, the SSR markers ICMP3018, PSMP2219, and PSMP2220 were used to distinguish CO 10 from the other varieties. Further, the CO10 variety produced additional alleles for all the markers due to its composite nature. Among the thirty-six SSR primers screened, none of them were found suitable to distinguish the TNAU hybrid CO 9 from its parents. The unique DNA fingerprints developed in the present study can be utilized for seed purity testing and varietal identification
June 2013 Update: Status Report on Assessment of Environmentally Assisted Fatigue for LWR Extended Service Conditions. Summary of 1. Room-Temperature Fatigue Test of 316 SS Specimens and Subsequent Data Analysis for Cyclic Plasticity Constitutive Model Development. 2. Other Ongoing Experimental and Mechanistic Modeling Activities.
EVALUATION OF IN VITRO ANTICANCER POTENTIAL OF ETHANOLIC EXTRACT AND ITS DIFFERENT FRACTIONS OF CAESALPINIA BONDUC (L) ROXB SEEDS
Objective: The present study aims to evaluate the anticancer potential of ethanolic extract and its different fractions of Caesalpinia bonduc seeds against Ehrlich Ascites Carcinoma (EAC) cell lines.
Methods: Ethanolic extract and its fractions were subjected to preliminary phytochemical screening and anticancer activity by using in vitro anticancer assay. Chemical constituents of petroleum ether fraction of C. bonduc seeds were identified by using GC-MS and the active compounds were subjected to in silico studies.
Results: In vitro anticancer assay showed that the petroleum ether fraction of ethanolic extract of Caesalpinia bonduc seeds has potential anticancer activity.
Conclusions: Petroleum ether fraction of ethanolic extract of Caesalpinia bonduc seeds has significant anticancer activity. Further in-depth studies, could result in the development of a good anticancer agent from the seeds of Caesalpinia bonduc
Beach Morphological Characteristics and Coastal Processes Along Dakshina Kannada Coast, West Coast of India
Source: ICHE Conference Archive - https://mdi-de.baw.de/icheArchiv
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Selection of non-adsorbing alkali components
This project consists of three phases of laboratory experimental study. In phase I (screening), eight candidate materials, 304SS (serves as a base material for comparison), Hastelloy C-276, Hastelloy X, Haynes No. 188, Allonized 304SS, Pt-coated 304SS, and ceramic-coated 304SS, will be subjected to atmospheric TGA study under the simulated PFBC (oxidizing) environment with and without alkali vapor doping. Each candidate material will be evaluated for its resistance toward alkali-vapor capture. In addition, a post-test metallographic characterization of the sample will be performed to obtain a better understanding of the alkali capture mechanism and material behavior. The material(s) with little or no alkali-vapor adsorption will be selected as the promising material(s) for the Phase II study. In Phase II, the promising material(s) will be further tested in the TGA under elevated pressure to simulate the PFBC environment (in terms of temperature, pressure, and gas composition). The effect of pressure on the extent of alkali-vapor adsorption will be evaluated, and the test samples will be metallographically characterized. The most promising candidate material(s) will be identified and recommended for further tesfing in the actual PFBC environment. In Phase III, four materials will be selected from the eight candidate materials screened in the PFBC environment and will be evaluated for their alkali-vapor capture by atmospheric TGA under the coal gasification fuel gas (reducing) environment. The tested samples will also be metallographically characterized. The most promising material(s) will be identified and recommended for further testing in the actual coal gasification environment
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